Proteomics study unveils ROS balance in acid-adapted Salmonella Enteritidis

Hu, Shuangfang; Yu, Yigang; Lv, Ziquan; Shen, Jianzhong; Ke, Yuebin; Xiao, Xi

doi:10.1016/j.fm.2020.103585

Cited by 18 publications

(19 citation statements)

References 47 publications

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“…77,78 Salmonella and E. coli O157:H7, common foodborne pathogens, threaten the health of millions of people every year. 79,80 It is of great significance to detect these two pathogens in food for food safety and human health purposes. Inspired by the unique properties of nanozymes, Lin, Xu, and coworkers designed a nanozyme-mediated dual immunoassay integrated with a smartphone platform for pathogen detection.…”

Section: Bacteriamentioning

confidence: 99%

The recent development of nanozymes for food quality and safety detection

Wang

et al. 2022

J. Mater. Chem. B

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Section: Bacteriamentioning

confidence: 99%

The recent development of nanozymes for food quality and safety detection

Wang

et al. 2022

J. Mater. Chem. B

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“…Similarly, S . Enteritidis altered the abundance of hundreds of proteins to respond to ethanol disinfectant and hydrochloric acid [ 14 , 15 ].…”

Section: Resultsmentioning

confidence: 99%

“…Analysis results by quantitative proteomics technology, such as tandem mass tags (TMT) and isobaric tags for relative and absolute quantification (iTRAQ), can provide key information on global physiological features of bacteria at the proteomic level [ 13 ]. This technique has been demonstrated to be powerful for the elucidation of stress response mechanisms in S. enterica to environmental stress factors (e.g., ethanol, heat, desiccation, acid, and ciprofloxacin) [ 14 , 15 , 16 , 17 ]. It is, therefore, expected that proteomic analysis will offer indispensable insight into the molecular bases of bacterial chlorine adaptation.…”

Section: Introductionmentioning

confidence: 99%

Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level

Dong

et al. 2022

Foods

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Salmonella Enteritidis (S. Enteritidis) can adapt to sublethal sodium hypochlorite conditions, which subsequently triggers stress resistance mechanisms in this pathogen. Hence, the current work aimed to reveal the underlying stress adaptation mechanisms in S. Enteritidis by phenotypic, proteomic, and physiological analyses. It was found that 130 ppm sodium hypochlorite resulted in a moderate inhibitory effect on bacterial growth and an increased accumulation of intracellular reactive oxygen species. In response to this sublethal treatment, a total of 492 proteins in S. Enteritidis showed significant differential abundance (p < 0.05; fold change >2.0 or <0.5), including 225 more abundant proteins and 267 less abundant proteins, as revealed by the tandem-mass-tags-based quantitative proteomics technology. Functional characterization further revealed that proteins related to flagellar assembly, two-component system, and phosphotransferase system were in less abundance, while those associated with ABC transporters were generally in more abundance. Specifically, the repression of flagellar-assembly-related proteins led to diminished swimming motility, which served as a potential energy conservation strategy. Moreover, altered abundance of lipid-metabolism-related proteins resulted in reduced cell membrane fluidity, which provided a survival advantage to S. Enteritidis. Taken together, these results indicate that S. Enteritidis employs multiple adaptation pathways to cope with sodium hypochlorite stress.

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“…Harsh situations or additional oxidative stress can damage the cell membrane; however, Salmonella can survive in harsh environments without any widespread damage to the membrane beneath by restricting the permeability of hydrophilic molecules by the closure of OmpA and OmpC porins. 44,70,71 roles in membrane integrity and are associated with developing antibiotic resistance in Salmonella. 14,46 During stress, such as the presence of disinfectants, reactive oxygen species (ROS), and antibiotics, OMPs like OmpX, OmpC, OmpA, and OmpD change their expression, which is directly associated with altering the cell membrane structure to help the bacteria survive.…”

Section: Resistance In Salmonellamentioning

confidence: 99%

Outer Membrane Proteins and Efflux Pumps Mediated Multi-Drug Resistance in Salmonella: Rising Threat to Antimicrobial Therapy

Akshay,

Deekshit,

Mohan Raj

et al. 2023

ACS Infect. Dis.

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Despite colossal achievements in antibiotic therapy in recent decades, drug-resistant pathogens have remained a leading cause of death and economic loss globally. One such WHO-critical group pathogen is Salmonella. The extensive and inappropriate treatments for Salmonella infections have led from multi-drug resistance (MDR) to extensive drug resistance (XDR). The synergy between efflux-mediated systems and outer membrane proteins (OMPs) may favor MDR in Salmonella. Differential expression of the efflux system and OMPs (influx) and positional mutations are the factors that can be correlated to the development of drug resistance. Insights into the mechanism of influx and efflux of antibiotics can aid in developing a structurally stable molecule that can be proficient at escaping from the resistance loops in Salmonella. Understanding the strategic responsibilities and developing policies to address the surge of drug resistance at the national, regional, and global levels are the needs of the hour. In this Review, we attempt to aggregate all the available research findings and delineate the resistance mechanisms by dissecting the involvement of OMPs and efflux systems. Integrating major OMPs and the efflux system’s differential expression and positional mutation in Salmonella may provide insight into developing strategic therapies for one health application.

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Proteomics study unveils ROS balance in acid-adapted Salmonella Enteritidis

Cited by 18 publications

References 47 publications

The recent development of nanozymes for food quality and safety detection

The recent development of nanozymes for food quality and safety detection

Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level

Outer Membrane Proteins and Efflux Pumps Mediated Multi-Drug Resistance in Salmonella: Rising Threat to Antimicrobial Therapy

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