Proteomics in commercial crops: An overview

Tan, Boon Chin; Lim, Yin Sze; Lau, Su-Ee

doi:10.1016/j.jprot.2017.05.018

Cited by 58 publications

(37 citation statements)

References 240 publications

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“…Due to the high complexity of living organisms in general and the plant proteome in particular, massspectrometric methods employed in its characterization need to be complemented by adequate separation techniques. Generally, based on the nature of these techniques, all bottom-up proteomic methods can be attributed to either gel-based or gel-free strategy (Tan, Lim, and Lau, 2017). The latter group of methods is also often referred to as liquid chromatography (LC)-based proteomics, although it also can rely on non-chromatographic techniques such as capillary electrophoresis (Chen, Cociorva, Norris, and Yates, 2017).…”

Section: Methodological Approaches In Seed Proteomicsmentioning

confidence: 99%

“…1): in the former, separation is done on the protein level (i.e. prior to enzymatic digestion), whereas in the latter, peptides are subjected to separation after enzymatic digestion (Tan, Lim, and Lau, 2017).…”

Section: Methodological Approaches In Seed Proteomicsmentioning

confidence: 99%

“…Mixing all three mixtures of protein derivatives in a 1:1:1 ratio gives direct access to protein expression (Unlu, Morgan, and Minde, 1997). The individual dyes contain different fluorophore moieties, so green, yellow and red fluorescence can be acquired from the same gel with minimal cross-talk and sensitivities of up to 150 pg per visualized electrophoretic zone (Tan, Lim, and Lau, 2017). Unfortunately, the DIGE approach does not allow reduction of inter-replicate variability or the personnelrelated bias.…”

Section: Plant Sciencementioning

confidence: 99%

See 2 more Smart Citations

Mining seed proteome: from protein dynamics to modification profiles

Frolov¹,

Мамонтова²,

Ihling³

et al. 2018

BioComm

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Section: Methodological Approaches In Seed Proteomicsmentioning

confidence: 99%

Section: Methodological Approaches In Seed Proteomicsmentioning

confidence: 99%

Section: Plant Sciencementioning

confidence: 99%

See 1 more Smart Citation

Mining seed proteome: from protein dynamics to modification profiles

Frolov¹,

Мамонтова²,

Ihling³

et al. 2018

BioComm

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“…Advanced techniques such as synchrotron radiation based techniques (e.g., X-ray absorption fine structure spectroscopy) combined with imaging techniques including scanning transmission X-ray microscopy (STXM) and micro X-ray fluorescence (µ-XRF), [122] and isotope labeling approaches [49,123] coupled with in situ detection techniques such as autoradiography, [100] Nano Secondary Ion Mass Spectrometry (NanoSIMS), [124] laser ablation inductively coupled plasma mass spectrometry (ICP-MS), [125] and single particle/cell ICP-MS are all sophistical techniques that can be brought to bare for studying ENMs transformation. Metabolomics, [126] proteomics, [127] exposomics, [128] and secretomics [129] are emerging powerful tools to identify the components of root exudates, EPS, and plant/microbial metabolite response to ENMs exposure. The datasets obtained could be combined with machine learning approaches (e.g., nanoinformatics) [130] to identify key drivers and critical steps of ENMs transformation, and to predict ENMs transformations thereby facilitating safe-by-design of ENMs for sustainable application in agriculture.…”

Section: Take Advantage Of the Emergence Of State Of The Art Analyticmentioning

confidence: 99%

Nanomaterial Transformation in the Soil–Plant System: Implications for Food Safety and Application in Agriculture

Zhang

Guo

Zhang

et al. 2020

Small

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Engineered nanomaterials (ENMs) have huge potential for improving use efficiency of agrochemicals, crop production, and soil health; however, the behavior and fate of ENMs and the potential for negative long‐term impacts to agroecosystems remain largely unknown. In particular, there is a lack of clear understanding of the transformation of ENMs in both soil and plant compartments. The transformation can be physical, chemical, and/or biological, and may occur in soil, at the plant interface, and/or inside the plant. Due to these highly dynamic processes, ENMs may acquire new properties distinct from their original profile; as such, the behavior, fate, and biological effects may also differ significantly. Several essential questions in terms of ENMs transformation are discussed, including the drivers and locations of ENM transformation in the soil–plant system and the effects of ENM transformation on analyte uptake, translocation, and toxicity. The main knowledge gaps in this area are highlighted and future research needs are outlined so as to ensure sustainable nanoenabled agricultural applications.

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“…Westermeier (2014) reported that separating proteins according to the combination of isoelectric focusing under denaturing conditions and sodium dodesil sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) delivers the highest resolution of all bio-analytic techniques. Tan et al (2017) reported that proteomics allows us to expansively analyze crop proteins and facilitate to understand the functions of the genes. MS can now identify a wide range of proteins and monitor their modulation during plant growth and development, as well as during responses to abiotic and biotic stresses.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of heat shock proteins in maize (Zea mays L.)

et al. 2019

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Background: Maize is one of the important cereal food crops in the world. High temperature stress causes adverse influence on plant growth. When plants are exposed to high temperatures, they produce heat shock proteins (HSPs), which may impart a generalized role in tolerance to heat stress. Proteome analysis was performed in plant to assess the changes in protein types and their expression levels under abiotic stress. The purpose of the study is to explore which proteins are involved in the response of the maize plant to heat shock treatment. Results:We investigated the responses of abundant proteins of maize leaves, in an Egyptian inbred line of maize "K1", upon heat stress through two-dimensional electrophoresis (2-DE) on samples of maize leaf proteome. 2-DE technique was used to recognize heat-responsive protein spots using Coomassie Brilliant Blue (CBB) and silver staining. In 2-D analysis of proteins from plants treated at 45°C for 2 h, the results manifested 59 protein spots (4.3%) which were reproducibly detected as new spots where did not present in the control. In 2D for treated plants for 4 h, 104 protein spots (7.7%) were expressed only under heat stress. Quantification of spot intensities derived from heat treatment showed that twenty protein spots revealed clear differences between the control and the two heat treatments. Nine spots appeared with more intensity after heat treatments than the control, while four spots appeared only after heat treatments. Five spots were clearly induced after heat treatment either at 2 h or 4 h and were chosen for more analysis by LC-MSMS. They were identified as ATPase beta subunit, HSP26, HSP16.9, and unknown HSP/Chaperonin. Conclusion:The results revealed that the expressive level of the four heat shock proteins that were detected in this study plays important roles to avoid heat stress in maize plants.

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Proteomics in commercial crops: An overview

Cited by 58 publications

References 240 publications

Mining seed proteome: from protein dynamics to modification profiles

Mining seed proteome: from protein dynamics to modification profiles

Nanomaterial Transformation in the Soil–Plant System: Implications for Food Safety and Application in Agriculture

Proteomic analysis of heat shock proteins in maize (Zea mays L.)

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