Proteomics in Aquaculture

Martín, Samuel

doi:10.1002/9780813807379.ch6

Cited by 3 publications

(1 citation statement)

References 88 publications

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“…Recent and very promising applications of proteomics have been provided in the field of aquaculture, such as the search for antigenic proteins, detection of differentially regulated proteins and the characterization of biologically active proteins, primarily to investigate the physiology, development biology and the impact of contaminants in aquatic organisms (Melamed et al. 2002; Saavedra & Bachère 2006; Martin 2009; Robalino et al. 2009; Forné et al.…”

Section: Introductionmentioning

confidence: 99%

Proteomics: present and future in fish, shellfish and seafood

Zhou

Ding

2012

Reviews in Aquaculture

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The aim of proteomics is to simultaneously resolve all potential proteins expressed by a cell, tissue or organism in a specific physiological condition. It will allow scientists to build and test better hypotheses, with the ultimate goal to find better solutions to challenges in agricultural sciences, medicine and environmental management. Recent and very promising applications of proteomics have also been provided in the field of aquaculture, such as the search for antigenic proteins, the detection of differentially regulated proteins and the characterization of biologically active proteins primarily to investigate the physiology, development biology and impact of contaminants in aquatic organisms. Thus, a brief overview of present classical proteomics methodology (electrophoresis and mass spectrometry) is presented, and the proteome technique will be focused in the context of applications beneficial to the field of fish, shellfish and seafood ⁄ seafood product. In addition, the future potential application and challenge of proteomics in aquaculture will also be discussed.

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Section: Introductionmentioning

confidence: 99%

Proteomics: present and future in fish, shellfish and seafood

Zhou

Ding

2012

Reviews in Aquaculture

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Exercise Physiology of Zebrafish: Swimming Effects on Skeletal and Cardiac Muscle Growth, on the Immune System, and the Involvement of the Stress Axis

Palstra

Schaaf

Planas

2012

Swimming Physiology of Fish

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A reference growth curve for nutritional experiments in zebrafish (Danio rerio) and changes in whole body proteome during development

Gómez‐Requeni

Conceição

Jordal

et al. 2010

Fish Physiol Biochem

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Zebrafish is one of the most used vertebrate model organisms in molecular and developmental biology, recently gaining popularity also in medical research. However, very little work has been done to assess zebrafish as a model species in nutritional studies in aquaculture in order to utilize the methodological toolbox that this species represents. As a starting point to acquire some baseline data for further nutritional studies, growth of a population of zebrafish was followed for 15 weeks. Furthermore, whole body proteome was screened during development by means of bi-dimensional gel electrophoresis and mass spectrometry. Fish were reared under best practice laboratory conditions from hatching until 103 days post-fertilization (dpf) and regularly fed ad libitum with Artemia nauplii from 12 dpf. A growth burst occurred within 9-51 dpf, reaching a plateau after 65 dpf. Fork length and body weight were significantly lower in males than in females from 58 dpf onwards. Proteomics analysis showed 28 spot proteins differently expressed through development and according to sex. Of these proteins, 20 were successfully identified revealing proteins involved in energy production, muscle development, eye lens differentiation, and sexual maturation. In summary, zebrafish exhibited a rapid growth until approximately 50 dpf, when most individuals started to allocate part of the dietary energy intake for sexual maturation. However, proteomic analysis revealed that some individuals reached sexual maturity earlier and already from 30 dpf onwards. Thus, in order to design nutritional studies with zebrafish fed Artemia nauplii, it is recommended to select a period between 20 and 40 dpf, when fish allocate most of the ingested energy for non-reproductive growth purposes.

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Proteomics in Aquaculture

Cited by 3 publications

References 88 publications

Proteomics: present and future in fish, shellfish and seafood

Proteomics: present and future in fish, shellfish and seafood

Exercise Physiology of Zebrafish: Swimming Effects on Skeletal and Cardiac Muscle Growth, on the Immune System, and the Involvement of the Stress Axis

A reference growth curve for nutritional experiments in zebrafish (Danio rerio) and changes in whole body proteome during development

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