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Previous studies from our laboratory have shown that salmon gonadotropins GTH I and GTH II have similar effects on testicular steroidogenesis in vitro. To determine whether the relative potencies of GTH I and GTH II changed during late stages of spermatogenesis, the effects of GTH I and GTH II on in vitro steroid production by testicular tissue from fish in stages IV to V of spermatogenesis were examined. Fragments of testicular tissue were incubated with GTH I and GTH II for 18 h at 15 degrees C. The in vitro production of 11-ketotestosterone (11-KT), testosterone (T), 17 alpha-hydroxyprogesterone (17OH-P), and 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P) was determined by RIA. GTH I and GTH II were equipotent in stimulating the production of 11-KT, T, and 17,20 beta-P (17OH-P was not detectable) by testicular tissue in stage IV of spermatogenesis. The sensitivity of testicular tissue to the steroidogenic effects of GTH II increased as spermatogenesis progressed. In contrast, the sensitivity of testicular tissue to the effects of GTH I on 17,20 beta-P production declined from stages IV to V, while the sensitivity of testicular tissue to the effects of GTH I on 11-KT production remained unchanged. These changes in sensitivity to GTH I and II are consistent with previously reported changes in GTH receptors in salmon testis and support the hypothesis that GTH I and GTH II may have different roles during spermatogenesis.

Music is known to have the power to induce strong emotions. The present study assessed, based on Electroencephalography (EEG) data, the emotional response of terminally ill cancer patients to a music therapy intervention in a randomized controlled trial. A sample of 40 participants from the palliative care unit in the Hospital del Mar in Barcelona was randomly assigned to two groups of 20. The first group [experimental group (EG)] participated in a session of music therapy (MT), and the second group [control group (CG)] was provided with company. Based on our previous work on EEG-based emotion detection, instantaneous emotional indicators in the form of a coordinate in the arousal-valence plane were extracted from the participants’ EEG data. The emotional indicators were analyzed in order to quantify (1) the overall emotional effect of MT on the patients compared to controls, and (2) the relative effect of the different MT techniques applied during each session. During each MT session, five conditions were considered: I (initial patient’s state before MT starts), C1 (passive listening), C2 (active listening), R (relaxation), and F (final patient’s state). EEG data analysis showed a significant increase in valence (p = 0.0004) and arousal (p = 0.003) between I and F in the EG. No significant changes were found in the CG. This results can be interpreted as a positive emotional effect of MT in advanced cancer patients. In addition, according to pre- and post-intervention questionnaire responses, participants in the EG also showed a significant decrease in tiredness, anxiety and breathing difficulties, as well as an increase in levels of well-being. No equivalent changes were observed in the CG.

Study objective-The aim was to assess the relationship between survival, tumour stage, and the interval from first symptom to diagnosis (SDI, or duration of symptoms).Design-This was a retrospective follow up study ofa cohort ofpatients registered in the tumour registry of the Hospital del Mar (Barcelona).Setting-Hospital based tumour registry, with patients derived mainly from the City of Barcelona.Participants-1247 cases of lung, breast, stomach, colon, or rectal cancer were analysed using survival curves and Cox proportional hazards regression. Subjects (mean age 63-6 years) were followed for a median length of 12-9 months after diagnosis. At the time of diagnosis one fourth of patients had disseminated disease.Measurements

The regulation of ovarian steroidogenesis in vitro by coho salmon FSH and LH was investigated in intact coho salmon follicles and isolated follicular layers at various stages of oocyte maturation, from late vitellogenesis until the completion of germinal vesicle breakdown (GVBD). In granulosa layers from all stages, LH, but not FSH, stimulated 17alpha,20beta-dihydroxy-4-pregnen-3-one (17, 20beta-P) production. In theca-interstitial layers from all stages, FSH and LH stimulated steroid production, LH being more potent than FSH. The basal steroid output of intact follicles was significantly lower than that of isolated follicular layers, and their response to FSH and LH also differed. First, the intact follicles produced 17alpha-hydroxyprogesterone in response to FSH during the central germinal vesicle stage while theca-interstitial layers did not. Second, estradiol-17beta production was not inhibited by LH during final oocyte maturation in intact follicles, as observed for granulosa layers. Our results indicate that LH is the determining factor regulating the production of the maturation-inducing steroid, 17,20beta-P, and the induction of GVBD in the salmonid ovary. In summary, we have provided evidence for maturation-associated changes in the effects of FSH and LH in the salmonid ovary, which further supports the hypothesis that FSH and LH have distinct functions in the teleost ovary.