Proteomics, functional characterization and antivenom neutralization of the venom of Pakistani Russell's viper ( Daboia russelii ) from the wild

Faisal, Tasnim; Tan, Kae Yi; Sim, Si Mui; Quraishi, Naeem H.; Tan, Nget Hong; Tan, Choo Hock

doi:10.1016/j.jprot.2018.05.003

Cited by 65 publications

(65 citation statements)

References 59 publications

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“…Comparatively, the high abundance of PLA 2 in both D. siamensis from Thailand (37.92%) and Indonesia (48.37%) is greater than that reported for D. siamensis from Guangxi, China (22.2%) and Taiwan (24.5%) [18]. The high abundance of PLA 2 is also comparable to that described for Russell's viper from Sri Lanka (35%), western India (32.5%) and Pakistan, where PLA 2 is lower in captive snakes (32.8%) than in wild-caught specimens (63.8%) [5][6][7][8].…”

Section: Discussionsupporting

confidence: 42%

“…Hence, the venom compositions can be highly variable even within the same species due to factors such as geographical, sexual and ontogenic variations [3,4]. It has been shown in the western Russell's viper (D. russelii) that the venoms from different geographical populations were variable in composition [5][6][7][8], and the variation could be the cause of the discrepancy in toxicity and antivenom efficacy. In the context of geographical variation impacting on venom composition, the extreme disjunctive distribution of Indonesian D. siamensis is of particular medical interest as it is isolated by more than 2000 km from the nearest conspecific populations in Thailand.…”

Section: Introductionmentioning

confidence: 99%

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Proteomics and antivenom immunoprofiling of Russell’s viper (Daboia siamensis) venoms from Thailand and Indonesia

Lingam

Tan

2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Section: Discussionsupporting

confidence: 42%

Section: Introductionmentioning

confidence: 99%

Proteomics and antivenom immunoprofiling of Russell’s viper (Daboia siamensis) venoms from Thailand and Indonesia

Lingam

Tan

2020

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Despite recognized intraspecies variations in the composition of venoms of Russell’s viper from different geographical origins, a common proteomic trend is the relative low percentage of SVMPs, although there is a notorious intraspecies variability 22 – 28 . Moreover, no hemorrhagic activity was observed in the skin and muscle of mice after injection of 20 µg of D. russelii venom from Pakistan 29 and this work. In contrast, the venom of B. asper has a high content of SVMPs 30 , some of which are hemorrhagic 31 , 32 , and induced evident hemorrhagic lesions in the skin and muscle of mice.…”

Section: Discussionsupporting

confidence: 42%

“…When analyzed by mass spectrometry, this peak contained, in addition to a snaclec, venom VEGF and a PLA 2 . Proteomic analyses of D. russelii venom from various geographical regions, including Pakistan, have shown the presence of VEGF 22 , 23 , 25 , 29 , in contrast with the venom of B. asper in which this component has not been reported 30 . Previous studies have demonstrated that VEGF from the venoms of various viperid species, including D. russelii , exerts a potent hypotensive effect and induces increments in vascular permeability in the skin at very low doses 37 – 39 .…”

Section: Discussionmentioning

confidence: 99%

Systemic vascular leakage induced in mice by Russell’s viper venom from Pakistan

Rucavado

Escalante

Camacho

et al. 2018

Sci Rep

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Envenomings by some populations of the Russell’s viper (Daboia russelii) are characterized by a systemic capillary leak syndrome (CLS) which causes hemoconcentration, and is associated with the severity of envenoming. We adapted a model of CLS in mice by assessing hemoconcentration. The venom of D. russelii from Pakistan, but not that of another viperid, Bothrops asper, induced hemoconcentration and an increment in vascular permeability, being devoid of hemorrhagic activity at the doses tested. These findings reveal a dichotomous pattern of vasculotoxicity in viperid snake venoms. This difference might depend on variations in venom composition, especially regarding metalloproteinases (SVMPs), which are low in Pakistani D. russelii and high in B. asper. Inhibition of SVMPs and phospholipases A2 in D. russelii venom did not abrogate hemoconcentration. An hemoconcentration-inducing fraction was obtained by chromatography, which contains vascular endothelial growth factor (VEGF), a known potent inducer of increment in vascular permeability. Exudates collected from tissue injected with venom also induced hemoconcentration, and the effect was inhibited by antivenom. However, the amount of venom in exudate required to induce the effect is low, as compared with venom dissolved in saline solution, hence suggesting that endogenous proteins present in the exudate, probably inflammatory mediators, potentiate the effect.

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“…As such, venom components are of wide interest to many research groups and have been the subject of extensive research. As about 90% of venom components are of peptide origin, all venom analyses use increasingly fast developing proteomic techniques, including electrophoresis [8][9][10][11], immunodetection techniques [12][13][14][15], different types of chromatography [16][17][18][19] and mass spectrometry [20][21][22][23]. Each proteomic method has specific requirements for both the sample components and the protein concentration range.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Methods for Measuring Protein Concentration in Venom Samples

Bocian

Sławek

Jaromin

et al. 2020

Animals

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Simple Summary: Snake venom is mostly composed of proteins and peptides, which are of interest to many researchers due to their potential pharmacological properties. Due to their biochemical character, these components are analyzed using proteomic techniques such as electrophoresis, chromatography and mass spectrometry. A very important stage of such studies is the measurement of protein concentration in the sample, which is most often performed by colorimetric methods. In the presented article, we used five such techniques on venoms of two snake species, namely Agkistrodon contortrix and Naja ashei. In the case of A. contortrix venom, four methods provide similar concentration values, whereas, in the case of N. ashei, the differences between results are very significant. The source of these differences should probably be seen in the differences in amino acid composition of proteins of these two venoms. With this report, we would like to draw attention to the need to select an appropriate method for measuring the concentration of protein in the venom, especially in the case of Elapid species.Abstract: Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity and high pharmacological potential. Much attention has been paid to the study of venom composition of different species and also detailed analysis of the properties of individual components. Since proteins and peptides are the active ingredients in venom, rapidly developing proteomic techniques are used to analyze them. During such analyses, one of the routine operations is to measure the protein concentration in the sample. The aim of this study was to compare five methods used to measure protein content in venoms of two snake species: the Viperids representative, Agkistrodon contortrix, and the Elapids representative, Naja ashei. The study showed that for A. contortrix venom, the concentration of venom protein measured by four methods is very similar and only the NanoDrop method clearly stands out from the rest. However, in the case of N. ashei venom, each technique yields significantly different results. We hope that this report will help to draw attention to the problem of measuring protein concentration, especially in such a complex mixture as animal venoms.

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Proteomics, functional characterization and antivenom neutralization of the venom of Pakistani Russell's viper ( Daboia russelii ) from the wild

Cited by 65 publications

References 59 publications

Proteomics and antivenom immunoprofiling of Russell’s viper (Daboia siamensis) venoms from Thailand and Indonesia

Proteomics and antivenom immunoprofiling of Russell’s viper (Daboia siamensis) venoms from Thailand and Indonesia

Systemic vascular leakage induced in mice by Russell’s viper venom from Pakistan

Comparison of Methods for Measuring Protein Concentration in Venom Samples

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