2020
DOI: 10.3390/ani10030448
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Abstract: Simple Summary: Snake venom is mostly composed of proteins and peptides, which are of interest to many researchers due to their potential pharmacological properties. Due to their biochemical character, these components are analyzed using proteomic techniques such as electrophoresis, chromatography and mass spectrometry. A very important stage of such studies is the measurement of protein concentration in the sample, which is most often performed by colorimetric methods. In the presented article, we used five s… Show more

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Cited by 14 publications
(5 citation statements)
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“…In this context, it seems that the venomics protocol for quantitative analysis, although laborious, remains the most accurate method available. Although the methods proposed therein are not without the limitations of quantitative methods, especially in the context of biases related to the presence of certain amino acids in protein sequences [19], the three-leveled quantification workflow still seems the best available solution [1].…”
Section: Discussionmentioning
confidence: 99%
“…In this context, it seems that the venomics protocol for quantitative analysis, although laborious, remains the most accurate method available. Although the methods proposed therein are not without the limitations of quantitative methods, especially in the context of biases related to the presence of certain amino acids in protein sequences [19], the three-leveled quantification workflow still seems the best available solution [1].…”
Section: Discussionmentioning
confidence: 99%
“…It is important to underline that we performed the protein measurement using Qubit assay. This type of quantification depends not only on the protein sequence, but it could be influenced by the changes in protein structure (Bocian et al., 2020), which could be induced by FBS heat inactivation. Future studies may help to explore the functional effects of the contaminates produced during the FBS heat inactivation and to define to what extent the effects of EVs on the recipient cells may be due to the FBS contaminants.…”
Section: Discussionmentioning
confidence: 99%
“…The lysate was added to ice homogenate, the supernatant was obtained after centrifugation, and put at -80°C for later use. The protein concentration of myocardial tissue in each group was calculated by bicinchoninic acid (BCA) assay (Bocian et al, 2020). The solution volume was calculated according to the sample concentration, and the protein was denatured by boiling after adding the sample buffer.…”
Section: Western Blot Analysismentioning
confidence: 99%