“…Hence, the proteomics study is very important to focus on the active products in the cells rather than gene expression study by using microarray and RT-PCR analysis 6,7 . Two-dimensional gel electrophoresis combined with mass spectrometry analysis has been frequently used in classical proteomics research to identify and functional characterization of the specific proteins from different organs and tissues of Bombyx mori such as collateral glands 8 , haemolymph [9][10][11] , fat body 12,13 , silk glands 14,15 , protharasic gland 16 , endocrine organs 17 , larval head, diapauses and non-diapauses egg and embryo [18][19][20] , integument, trachea and adult scale 21 , midgut and peritrophic membrane [22][23][24][25] , larval gonads and testis 26,27 , Malpighian tubules 28 , wing discs 29 and newly hatched larva 30 . Further, proteome profiling of several organs and tissues analysis revealed that these proteins play a crucial role in the nervous system development, digestion, protein synthesis, transport of ions and O 2 , reproduction, stress response and apoptosis in silkworm Bombyx mori.…”