Comparative Proteomic Analysis of Larva and Adult Heads of Silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Abstract

Background: The complete understanding of feeding and reproduction strategies during metamorphosis of silkworm Bombyx mori is very essential, for that a comparative proteomics analysis was used to investigate the proteins extracted from the head of the larva and adult of Bombyx mori. Materials and Methods: Proteins were separated and identified by using 2D-PAGE and MALDI-TOF-TOF-MS analysis respectively. The functions of each proteins were annotated using STRAP GO analysis. The expression level of each protein spots between larvae and adult were analyzed using 2D image platinum 7. Results: Using 2-DE gels stained with coomassie staining revealed, 315±3 and 296±3 proteins spots with pI ranges of 4-10 and 3.5-8 in the larva and adult head, respectively. Thirty proteins were identified based on differential and unique expression in the head of the larva and adult of B. mori. Among the 30 identified proteins, comparatively 10 proteins were up regulated and 6 proteins were down regulated in the larvae, whereas 6 proteins were up regulated and 10 were down regulated in the adult. In addition, 6 and 8 unique proteins expressions were observed in the larvae and adult, respectively. Database search combined with STRAP GO analysis revealed that few up regulated and unique protein in the larvae such as odorant binding proteins, argonaute protein, apolipophorin III precursor, enolase and iron-ion transport proteins were localized to regulate the feeding activity of larva. Furthermore, some proteins are highly and uniquely expressed from adult head such as glycoprotein hormone, N-acetyltranferase, cytochrome p450, DNA binding and intracellular transport protein, clathrin coat assembly protein and metabolic enzyme which may regulate adult ecolsion and reproduction. Conclusion: The results suggest and confirmed that the protein expression in each stage could alter the behaviors of the insect related to larval feeding, growth and reproduction in the adult. Further the characterization and functions of other proteins identified are discussed. This study will help to improve the silk production which leads to economy improvement in sericulture industry.

Novel proteinase inhibitor from the hemolymph of soybean pest Anticarsia gemmatalis (lepidóptera: Noctuidae): Structural modeling and enzymatic kinetic

“…Thus, was identified a serpin inhibitor with a molecular mass of 38 ± 2 kDa. This mass coincides with other inhibitors serpin-like, Despite the variations in size, the inhibitors of this family have an approximate molecular mass of 40-50 kDa (Arunprasanna et al, 2017;Christen et al, 2012;Ragan et al, 2010;Toubarro et al, 2013;Xu et al, 2015).…”