Proteomics: a new approach to the study of disease

Chambers, G.; Lawrie, Laura; Cash, Phillip; Murray, Graeme I.

doi:10.1002/1096-9896(200011)192:3<280::aid-path748>3.0.co;2-l

Cited by 192 publications

(92 citation statements)

References 69 publications

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“…The identification, quantification, classification, and functional assignment of proteins will be essential to the full understanding of these molecular events. Such information will likely prove to be crucial in cancer prognosis, diagnosis, prevention and therapy with the ultimate goods being therapeutic target discovery, rational drug design and the identification of early detection surrogate biomarkers (12,13).…”

Section: Discussionmentioning

confidence: 99%

The Proteomics Approach to Find Biomarkers in Gastric Cancer

et al. 2003

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Gastric cancer is a very serious disease and is naturally resistant to many anticancer drugs. To reduce the mortality and improve the effectiveness of therapy, many studies have tried to find key biomarkers. Proteomic technologies are providing the tools needed to discover and identify disease-associating biomarkers. The proteomic study of gastric cancer establishes any specific events that lead to cancer, and it provides a direct way to define the true function of genes. Using two dimensional (2-D) electrophoresis of the stomach cancer tissue, we have gained about 1,500 spots in each gel, and 140 protein spots also were identified. Among the identified proteins, there were seven over-expressed proteins in stomach cancer tissue: NSP3, transgelin, prohibitin, heat shock protein (hsp) 27 and variant, protein disulfide isomerase A3, unnamed protein product and glucose regulated protein. There were also seven under-expressed proteins in stomach cancer: Apolipoprotein A-1, p20, nucleoside diphosphate isomerase A, alpha 1 antitrypsin, desmin, serum albumin and serotransferrin.

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Section: Discussionmentioning

confidence: 99%

The Proteomics Approach to Find Biomarkers in Gastric Cancer

et al. 2003

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“…Two-dimensional electrophoresis (2-DE) is the principal tool in proteomics, which can analyse thousands of proteins in one experiment, providing the highest resolution in protein separation. By comprehensively examining the protein expression profiles in normal and diseased samples via 2-DE, proteomics provides information on new biomarkers, disease-associated targets, and pathogenesis (Alaiya et al, 2000;Chambers et al, 2000;Miklos and Maleszka, 2001). This technique has been used extensively to investigate various diseases, including SCC of the oesophagus (Zhang et al, 2003), bladder (Ostergaard et al, 1997;Celis et al, 1999Celis et al, , 2000, lung (Li et al, 2003a(Li et al, , b, 2004, and head and neck (Wu et al, 2002;Drake et al, 2005;Roesch Ely et al, 2005).…”

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confidence: 99%

Ubiquitous mitochondrial creatine kinase downregulated in oral squamous cell carcinoma

et al. 2006

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In this study, we performed two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionisation time of fly mass spectrometry to identify the protein(s) associated with the development of oral squamous cell carcinomas (OSCCs) by comparing patterns of OSCC-derived cell lines with normal oral keratinocytes (NOKs), and found that downregulation of ubiquitous mitochondrial creatine kinase (CKMT1) could be a good candidate. Decreased levels of CKMT1 mRNA and protein were detected in all OSCC-derived cell lines examined (n ¼ 9) when compared to those in primary normal oral keratinocytes. Although no sequence variation in the coding region of the CKMT1 gene with the exception of a nonsense mutation in exon 8 was identified in these cell lines, we found a frequent hypermethylation in the CpG island region. CKMT1 expression was restored by experimental demethylation. In addition, when we transfected CKMT1 into the cell lines, they showed an apoptotic phenotype but no invasiveness. In clinical samples, high frequencies of CKMT1 downregulation were detected by immunohistochemistry (19 of 52 (37%)) and quantitative real-time RT -PCR (21 of 50 (42%)). Furthermore, the CKMT1 expression status was significantly correlated with tumour differentiation (Po0.0001). These results suggest that the CKMT1 gene is frequently inactivated during oral carcinogenesis and that an epigenetic mechanism may regulate loss of expression, which may lead to block apoptosis.

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“…Densitometric analysis of each spot was calculated using albumin as a reference value, as reported. 17 In previous works, we and others have identified by mass spectrometry some of the here-reported proteins. 12,18,19 However, in order to confirm the identity of some of the here-measured plasma proteins, we have identified again some of these proteins by mass spectrometry.…”

Section: Resultsmentioning

confidence: 73%

Relationship between Vitamin D Binding Protein and Aspirin Resistance in Coronary Ischemic Patients: A Proteomic Study

et al. 2007

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Our aim was to analyze the plasma proteome in aspirin (acetylsalicylic acid [ASA])-sensitive and ASA-resistant coronary ischemic patients. Plasma from 19 ASA-sensitive and 19 ASA-resistant patients was analyzed. For the proteomic study, two-dimensional electrophoresis was performed. The expression of one isotype of the fibrinogen γ chain and three isotypes of haptoglobin was increased in ASA-resistant patients. Three vitamin D binding protein isotypes were increased in ASA-resistant patients. In vitro incubation of vitamin D binding protein (DBP) with blood from healthy volunteers reduced the inhibitory effect of ASA on thromboxane A2 production. DBP may be a new regulator of the inhibitory effect of ASA on platelets. Keywords: Aspirin (acetylsalicylic acid [ASA]) · plasma proteome · platelets · thromboxane A2

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Proteomics: a new approach to the study of disease

Cited by 192 publications

References 69 publications

The Proteomics Approach to Find Biomarkers in Gastric Cancer

The Proteomics Approach to Find Biomarkers in Gastric Cancer

Ubiquitous mitochondrial creatine kinase downregulated in oral squamous cell carcinoma

Relationship between Vitamin D Binding Protein and Aspirin Resistance in Coronary Ischemic Patients: A Proteomic Study

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