Relationship between Vitamin D Binding Protein and Aspirin Resistance in Coronary Ischemic Patients:  A Proteomic Study

Abstract

Our aim was to analyze the plasma proteome in aspirin (acetylsalicylic acid [ASA])-sensitive and ASA-resistant coronary ischemic patients. Plasma from 19 ASA-sensitive and 19 ASA-resistant patients was analyzed. For the proteomic study, two-dimensional electrophoresis was performed. The expression of one isotype of the fibrinogen γ chain and three isotypes of haptoglobin was increased in ASA-resistant patients. Three vitamin D binding protein isotypes were increased in ASA-resistant patients. In vitro incubation of vitamin D binding protein (DBP) with blood from healthy volunteers reduced the inhibitory effect of ASA on thromboxane A2 production. DBP may be a new regulator of the inhibitory effect of ASA on platelets. Keywords: Aspirin (acetylsalicylic acid [ASA]) · plasma proteome · platelets · thromboxane A2

Changes in the expression of plasma proteins associated with thrombosis in BRCA1 mutation carriers

“…As reported, densitometric analysis of each spot was calculated using albumin as a reference value (Mateos-Cáceres et al 2004). In previous works, we and others have identiWed by mass spectrometry some of the here-reported proteins (Alonso-Orgaz et al 2006;López-Farré et al 2007;Koomen et al 2005;Hlavaty et al 2003). However, in order to conWrm the identity of some of the here-measured plasma proteins, we identiWed again some of the studied plasma proteins by mass spectrometry (data not shown).…”

“…As reported (Mateos-Cáceres et al 2004;Alonso-Orgaz et al 2006;López-Farré et al 2007), 500 g of total plasma proteins, determined by bicinchoninic acid reagent (Pierce), were homogenized in a lysis buVer containing Bio-Lyte TM ampholytes (Bio-Rad). Plasma samples were loaded on immobilized pH gradient IPG strips (pH 4-7), and isoelectric focusing was performed using a Protean IEF cell system (Bio-Rad Labs).…”

“…Image analysis was carried out using Quantity One 4.2.3 (Bio-Rad Labs). Each spot intensity volume was processed by background subtraction, and total spot volume was normalized by the corresponding spot volume of albumin (Mateos-Cáceres et al 2004;Alonso-Orgaz et al 2006;López-Farré et al 2007). The spots whose intensity volume showed signiWcant diVerences among experimental groups were identiWed by mass spectrometry.…”

Correlation between platelet gelsolin levels and different types of coronary heart disease

“…Interestingly, in this study we observed that the plasma of CHD patients contains greater amounts of Gc-globulin compared with healthy subjects. Previous studies [25] demonstrated that three vitamin D binding protein isotypes were increased in asprin-resistant CHD patients and this could reduce the inhibitory effect of asprin on thromboxane A 2 production. Thus, this may suggest that the increased Gc-globulin level in this study may be associated with a large number of asprin-resistant patients included in our study or with increased reactively due to sudden severe depletion.…”

Overview of Current Proteomic Approaches for Discovery of Vascular Biomarkers of Atherosclerosis

“…Moreover, differential proteomic analysis has been addressed to elucidate effects on plasma proteome of different pharmacological treatments (AlonsoOrgaz et al, 2006;López-Farré et al, 2007). In this regard, the most applied proteomic methodologies were gel electrophoresis coupled to mass spectrometry analyses followed by gel free proteomic approaches such as LC MS/MS (Donahue et al, 2006;Wilson et al, 2007;Prentice et al, 2010) and microarrays (Tabibiazar et al, 2006).…”