The Proteomics Approach to Find Biomarkers in Gastric Cancer

Ryu, Jin Woo; Kim, Hyung Jee; Lee, Young Sun; Myong, Na Hye; Hwang, Cheol Hoh; Lee, Gae Sung; Yom, Heng Cherl

doi:10.3346/jkms.2003.18.4.505

Cited by 127 publications

(108 citation statements)

References 12 publications

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“…Transgelin 2, which has been reported to be overexpressed in gastric cancer (Ryu et al, 2003), was found to be upregulated in 11 of 16 HCC patients. This protein is thought to be involved in cell proliferation and migration, suggesting that its overexpression may be implicated in tumour progression.…”

Section: Discussionmentioning

confidence: 95%

Identification and analysis of tumour-associated antigens in hepatocellular carcinoma

Wang

et al. 2005

Br J Cancer

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To identify tumour and tumour-associated antigens in patients with hepatocellular carcinoma (HCC) one may find potential diagnostic markers and immunotherapeutic targets. In the current study, 30 distinct antigens reactive with serum IgG from HCC patients were identified by serological analysis of cDNA expression libraries (SEREX). The mRNA expression patterns of 14 of these 30 antigens were altered in cancer as further revealed by cDNA microarray, with upregulation for nine and downregulation for five antigens. One of the upregulated antigens was cancer-testis (CT) antigen (CAGE), which had been previously reported to be expressed exclusively in normal gametogenic tissues and aberrantly expressed in a variety of cancer cells. In our study, CAGE mRNA was expressed in 39.4% of HCC patients, 73.3% of patients with gastric cancer and 30.8% of patients with colorectal cancer. Antibodies against CAGE protein were detected in approximately 5.1% of the sera from HCC patients, 8.3% of that from gastric cancer patients and 7.3% of that from colorectal cancer patients. The relative high incidence of CAGE in cancer cells makes it a potential target for vaccine design. Another antigen of great interest is transgelin 2. The overexpression of transgelin 2 mRNA in a large per cent (69%) of HCC points to its potential as a diagnostic marker for HCC.

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Section: Discussionmentioning

confidence: 95%

Identification and analysis of tumour-associated antigens in hepatocellular carcinoma

Wang

et al. 2005

Br J Cancer

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“…The over-expression of HSP27 in gastric cancer has been observed by several studies (8,25,26). In gastric cancer, over-expression of HSP27 may be associated with metastasis to the lymph nodes, advanced stage (III and IV) cancer, and shorter overall survival of patients (27,28), indicating that HSP27 has prognostic significance for gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

Heat shock protein 27 is over-expressed in tumor tissues and increased in sera of patients with gastric adenocarcinoma

Huang¹,

Ye²,

Huang

et al. 2009

Clinical Chemistry and Laboratory Medicine

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Background: In a previous study, we found that heat shock protein 27 (HSP27) was over-expressed in gastric adenocarcinoma (GA) tissue. In this study, our goal was to further verify the expression profile of HSP27 in patients with GA. Methods: Western blot and immunohistochemistry were employed to determine HSP27 expression in 50 paired tumor and adjacent normal tissue. ELISA was used to quantify serum HSP27 concentrations in the same 50 GA patients and 50 healthy individuals. Results: Compared to adjacent normal tissues, HSP27 was over-expressed in 25 (50%, ps0.000) and 24 (48%, ps 0.000) cases of GA tissue by Western blot and immunohistochemistry, respectively. ELISA revealed significantly higher serum concentrations of HSP27 in patients with GA patients (means986 pg/mL) compared to healthy individuals (means573 pg/mL) (ps0.003). In addition, infection with Helicobacter pylori (HP) in healthy individuals was associated with increased expression of HSP27 in both gastric mucosa and serum. Conclusions: These data suggest that HSP27 is overexpressed in GA tissue and serum concentrations of HSP27 are increased in patients with GA. Over-expression of HSP27 may indicate a gastric malignant/infectious process. The detection of serum HSP27 concentrations by ELISA may be useful for screening for GA. Clin Chem Lab Med 2010;48:263-9.

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“…Transgelin, also known as SM22-α, of the calciumbinding protein family, is an actin-binding protein with a molecular weight of 22 kda. Transgelin was overexpressed in gastric cancer and renal cell carcinoma, and was capable of inducing igG responses in vivo in some patients (10)(11)(12). in order to identify a potential diagnostic marker for liver cancer and immunotherapy molecular targets, Shi et al (13) detected tumor antigens and tumor-associated antigens in patients with Hcc by serological analysis of cdna expression libraries (SereX).…”

Section: Discussionmentioning

confidence: 99%

Hepatocellular carcinoma-associated protein markers investigated by MALDI-TOF MS

Xiao-li

Zhou²,

Yang³

et al. 2010

Mol Med Rep

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Abstract. Hepatocellular carcinoma (Hcc) is one of the most common types of cancer worldwide. The initial hepatocellular alterations that precede the appearence of Hcc include chronic viral hepatitis/cirrhosis, foci of phenotypically altered hepatocytes and, subsequently, dysplastic hepatocytes that form foci and nodules. These changes cause a discrepancy in the microenvironment of liver cells, which may result in changes in the protein expression profile of the cells. The aim of the present study was to investigate differences between the protein expression profiles at various stages of liver disease in order to better understand the mechanisms of Hcc and to identify potential biomarkers for its early diagnosis. The proteins of specific cells were obtained from Hcc tissue sections and pre-cancerous lesions using a manual microdissection technique, and were investigated by a two dimensional gel electrophoresis (2-de) Maldi-ToF MS proteomics approach. Select identified proteins were reconfirmed by immunohistochemistry. A total of 95 differentially expressed proteins, with an over 2-fold disparity in expression levels between cells of varying morphology during the stages of hepatocarcinogenesis, were detected by 2-de. among these 95 proteins, 80 were determined to be involved in numerous cell functions, including cell growth and proliferation, protein synthesis and metabolism, apoptosis and signal transduction. These identified proteins, which include stratifin (14-3-3), transgelin 2, heat-shock protein (HSP)70, HSP27, manganese superoxide dismutase, prohibitin, dJ1, α-enolase, peroxiredoxin 6, aldo-keto reductase family member B10, phosphoglycerate kinase 1, α-1-antitrypsin and nm23-H1, may play a role in the development of Hcc. Protein expression profiles differed markedly between the HCC tissue samples and pre-cancerous lesions, suggesting that alterations in protein expression occurred frequently during the process of hepatocarcinogenesis. analysis of the differential expression of proteins related to the development of Hcc may help elucidate the molecular mechanisms of the disease. These proteins may also serve as candidate biomarkers for early Hcc diagnosis. IntroductionHepatocellular carcinoma (Hcc) is one of the world's most common malignancies. The majority of Hcc cases are associated with chronic hepatitis or cirrhosis. Pathological changes in Hcc are involved in liver cell degeneration and necrosis, inflammatory cell infiltration, hepatocyte regeneration and fibrous tissue proliferation. During the development of Hcc, the liver undergoes a series of morphological changes, including chronic viral hepatitis/cirrhosis, foci of phenotypically altered hepatocytes and atypical hyperplasia (1-4). These changes result in an altered microenvironment, in which the intracellular protein expression pattern is changed as well.The proteomics approach allows for the analysis of changes in the protein expression profile of cells during disease progression. By comprehensively analyzing changes in protein expression patt...

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The Proteomics Approach to Find Biomarkers in Gastric Cancer

Cited by 127 publications

References 12 publications

Identification and analysis of tumour-associated antigens in hepatocellular carcinoma

Identification and analysis of tumour-associated antigens in hepatocellular carcinoma

Heat shock protein 27 is over-expressed in tumor tissues and increased in sera of patients with gastric adenocarcinoma

Hepatocellular carcinoma-associated protein markers investigated by MALDI-TOF MS

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