Proteomic profiling of royal jelly produced by Apis mellifera L. exposed to food containing herbicide-based glyphosate

Faita, Márcia Regina; Chaves, Adriana; Corrêa, Caio Cézar Guedes; Silveira, Vanildo; Nodari, Rubens Onofre

doi:10.1016/j.chemosphere.2021.133334

Cited by 8 publications

(4 citation statements)

References 49 publications

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“…The reduction in the brood area may be related to different changes in the hive, including: 1) lack of care for the larvae by nursing bees, evidenced by the non-removal of dead pupae and changes in hygienic behavior (Cardozo, 2017); 2) nutritional deficiency due to the reduction of workers to collect resources for the hive, in addition to the early degeneration of the hypopharyngeal glands of the nursing bees (Faita et al, 2018) and reduction in the production of royal jelly (Chaves et al, 2020); 3) impairment of social immunity in the hive due to changes in the proteomic profile of royal jelly with reduced production of MRJP3, the main immunity protein of A. mellifera (Faita et al, 2022).…”

Section: Evaluation Of the Strength Of The Hivesmentioning

confidence: 99%

“…At the same time, a commercial formulation based on glyphosate caused higher mortality in bees infected with Nosema spp. (Faita et al, 2020), ultrastructural alterations of the hypopharyngeal glands (Faita et al, 2018), reduction in royal jelly production and its proteomic profile (Faita et al, 2022;Chaves et al, 2020). Additionally, commercial formulations of herbicides based on glyphosate (HBG) have adjuvants and inert ingredients, which can be even more toxic to bees than the active ingredients (Mullin et al, 2016;Mesnage et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Glyphosate residue in honey and impacts on Africanized bee hives under field conditions

Faita

Alves

Micke

et al. 2023

dataset

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Honey and other bee products may contain residues of different substances, including pesticides, which is considered a public health problem. In addition, they characterize risks to the health of Apis mellifera, which have been showing an increasing decline in their populations. There are many protocols for identifying pesticides in bee products which, in general, are complex matrices whose results of routine investigations in control laboratories are rarely disclosed. In this sense, the objective of the present study was to determine the presence of residues of glyphosate and its metabolite aminomethylphosphonic acid (AMPA) in honey, as well as its effect on the strength of the hive of A. mellifera. Samples were collected from hives experimentally exposed to food containing a sublethal dose of Roundup® and conducted by hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry (HILIC-MS/MS). The AMPA content was lower than the method’s detection limit in honey samples from hives that received food containing the herbicide. It was possible to quantify glyphosate one week after the last artificial feeding (R1 8.45 ± 1.09 µg g−1; R2 8.15 ± 2.14 µg g−1; R3 23.90 ± 2.95 µg g−1). In a hive sample fed for more than four weeks, glyphosate was present in lower concentrations (3.12 ± 0.89 µg g−1) with no detection of AMPA. From the analysis of the strength of the hives, we observed a decrease in the population of adult individuals and the brood area, the absence of a queen, and no construction of royal cells by the workers in the hives of the Roundup® treatment in comparison to the control group, in which the hives remained with queen size, high adult and brood population, and food stock. Although present, glyphosate did not undergo degradation in honey during the evaluated period. Thus, we could infer that the presence of Roundup® in bee feed may be present in honey, representing a risk to consumers’ health and economic damage to beekeepers. This is the first study that evaluated the effect of glyphosate residues on hive strength, contributing to the understanding of the Roundup® mode of action in different aspects that affect the survival of colonies under field conditions.

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Section: Evaluation Of the Strength Of The Hivesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Glyphosate residue in honey and impacts on Africanized bee hives under field conditions

Faita

Alves

Micke

et al. 2023

dataset

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“…Phosphoproteome study of honey bee products such as royal jelly and venom is critical to exploring their biochemical and functional properties. Royal jelly is queen food for the entire life and is high in defensive components linked to collective immunological defenses [37]. The phosphoproteome analysis of royal jelly between honey bee species showed diverse biological characteristics.…”

Section: Molecular Insight Into Biochemistry and Function Of Honey Be...mentioning

confidence: 99%

New insight into phosphoproteome research improves the in-depth understanding of honey bee biology

Ahmad¹,

Li²

2022

SciRevs.Biology

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Protein phosphorylation is essential in a variety of biological activities. Notably, phosphoproteomics has opened new possibilities for honey bee biological study at the molecular and biochemical levels, considering the advancements in LC-MS/MS apparatus and computational analysis. This review extensively evaluated recent advances in honey bee biology utilizing phosphoproteomics methodologies in terms of progressive physiology, age polytheism, and biological changes in some organs, tissues, and cells. Furthermore, a comprehensive phosphoproteomic is necessary for new insight into honey bee biology, a better understanding of the research subject, and determining prospective future research areas.

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“…Very little is known about the sublethal effects of GLY on bee social immunity. A recent study showed that the presence of formulated GLY in the diet of A. mellifera can interfere with the protein composition of royal jelly, impairing the nutrition and social immunity of these animals (Faita, Chaves, Corrêa, Silveira & Nodari, 2022).…”

Section: Alterations In the Immunity Of Beesmentioning

confidence: 99%

Review on the sublethal effects of pure and formulated glyphosate on bees: Emphasis on social bees

Battisti

Potrich

Lozano

et al. 2022

J Applied Entomology

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Bees are declining worldwide, and the use of pesticides has been linked to this problem. Studies show that even herbicides can negatively impact bees, causing death or compromising health. As a result, concern about the use of glyphosate (GLY) has increased, as it is the most sold pesticide. Studies have shown that exposure of bees to GLY can trigger sublethal effects. Considering the speed with which information is published, reviews become important for the integration of knowledge, aiding understanding of the topic. Therefore, the present study aimed to review the literature on the sublethal effects of GLY and the different commercial formulations on bees.After the literary review, it was observed that the exposition, acute and chronic, of larvae and adults of social and solitary bees, to GLY and its formulations, can trigger alterations in gene expression, enzyme functioning, oxidative metabolism, cell/tissue structure, intestinal microbiota diversity, learning, food consumption, flight and vertical displacement capacity, circadian cycle and body development of these insects.The most used species in the studies was Apis mellifera L. Studies are still necessary to understand the sublethal effects of GLY on bees, in the medium and long term, on colony homeostasis, especially about the information on the toxicity of some surfactants present in the different commercial formulations.

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Proteomic profiling of royal jelly produced by Apis mellifera L. exposed to food containing herbicide-based glyphosate

Cited by 8 publications

References 49 publications

Glyphosate residue in honey and impacts on Africanized bee hives under field conditions

Glyphosate residue in honey and impacts on Africanized bee hives under field conditions

New insight into phosphoproteome research improves the in-depth understanding of honey bee biology

Review on the sublethal effects of pure and formulated glyphosate on bees: Emphasis on social bees

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