Proteomic Profiling of Retinoblastoma-Derived Exosomes Reveals Potential Biomarkers of Vitreous Seeding

Galardi, Angela; Colletti, Marta; Lavarello, Chiara; Paolo, Virginia Di; Mascio, Paolo Di; Russo, Ida; Cozza, Raffaele; Romanzo, Antonino; Valente, Paola; Vito, Rita De; Pascucci, Luisa; Peinado, Héctor; Carcaboso, Ángel M.; Petretto, Andrea; Locatelli, Franco; Giannatale, Angela Di

doi:10.3390/cancers12061555

Cited by 33 publications

(35 citation statements)

References 127 publications

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“…A growing body of evidence suggests that sEVs might be useful as biomarkers in many diseases [ 102 , 104 , 105 , 106 ], as well as prognostic agents providing information about the phase of disease or predicted therapy outcomes [ 107 , 108 , 109 ]; sEVs transfer specific cargo components depending on the cell of origin, and ensure a safe environment for transported compounds. This allows their delivery to both neighboring and distant cells, which is an important modality of communication between tissues and organs.…”

Section: Resultsmentioning

confidence: 99%

The Emerging Role of Small Extracellular Vesicles in Inflammatory Airway Diseases

et al. 2021

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Extracellular vesicles (EVs) are produced and released by all cells and are present in all body fluids. They exist in a variety of sizes, however, small extracellular vesicles (sEVs), the EV subset with a size range from 30 to 150 nm, are of current interest. By transporting a complex cargo that includes genetic material, proteins, lipids, and signaling molecules, sEVs can alter the state of recipient cells. The role of sEVs in mediating inflammatory processes and responses of the immune system is well-documented, and adds another layer of complexity to our understanding of frequent diseases, including chronic rhinosinusitis (CRS), asthma, chronic obstructive pulmonary disease (COPD), and upper airway infections. In these diseases, two aspects of sEV biology are of particular interest: (1) sEVs might be involved in the etiopathogenesis of inflammatory airway diseases, and might emerge as attractive therapeutic targets, and (2) sEVs might be of diagnostic or prognostic relevance. The purpose of this review is to outline the biological functions of sEVs and their capacity to both augment and attenuate inflammation and immune response in the context of pathogen invasion, CRS, asthma, and COPD.

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Section: Resultsmentioning

confidence: 99%

The Emerging Role of Small Extracellular Vesicles in Inflammatory Airway Diseases

et al. 2021

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“…Moreover, tumoral exosomes can induce drug resistance carrying miRNAs that target antiapoptotic and immune-suppressive pathways or ABC transporters, which export chemotherapeutic agents out of recipient cells ( Santos and Almeida, 2020 ). Given their involvement in cancer progression and their presence in different biological fluids, there have been increasing efforts toward their characterization as a source of possible diagnostic and prognostic biomarkers even in pediatric oncology ( Colletti et al, 2017 , 2019 , 2020 ; Galardi et al, 2019 , 2020 ) and as a delivery tool for biomedical applications ( Rezaie et al, 2018 ; Rahbarghazi et al, 2019 ; Jabbari et al, 2020b ; Wu Z. et al, 2020 ).…”

Section: Exosomes: Biogenesis Release and Functionsmentioning

confidence: 99%

Autophagy and Exosomes Relationship in Cancer: Friends or Foes?

Colletti

Ceglie

Giannatale

et al. 2021

Front. Cell Dev. Biol.

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Autophagy is an intracellular degradation process involved in the removal of proteins and damaged organelles by the formation of a double-membrane vesicle named autophagosome and degraded through fusion with lysosomes. An intricate relationship between autophagy and the endosomal and exosomal pathways can occur at different stages with important implications for normal physiology and human diseases. Recent researches have revealed that extracellular vesicles (EVs), such as exosomes, could have a cytoprotective role by inducing intracellular autophagy; on the other hand, autophagy plays a crucial role in the biogenesis and degradation of exosomes. Although the importance of these processes in cancer is well established, their interplay in tumor is only beginning to be documented. In some tumor contexts (1) autophagy and exosome-mediated release are coordinately activated, sharing the molecular machinery and regulatory mechanisms; (2) cancer cell-released exosomes impact on autophagy in recipient cells through mechanisms yet to be determined; (3) exosome-autophagy relationship could affect drug resistance and tumor microenvironment (TME). In this review, we survey emerging discoveries relevant to the exosomes and autophagy crosstalk in the context of cancer initiation, progression and recurrence. Consequently, we discuss clinical implications by targeting autophagy-exosomal pathway interaction and how this could lay a basis for the purpose of novel cancer therapeutics.

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“…Of these, 25 are upregulated, and eight are downregulated [ 112 , 113 ]. In another study by Galardi A et al, the proteomic profiling of the exosomes constituents has identified 99 proteins that are exclusively present in RB tumors [ 114 ]. We have analyzed the targets of the miRNAs from both these studies [ 112 , 113 ] from the proteins identified in RB tumor exosomes [ 114 ].…”

Section: Mirna and Their Targets In Retinoblastomamentioning

confidence: 99%

“…In another study by Galardi A et al, the proteomic profiling of the exosomes constituents has identified 99 proteins that are exclusively present in RB tumors [ 114 ]. We have analyzed the targets of the miRNAs from both these studies [ 112 , 113 ] from the proteins identified in RB tumor exosomes [ 114 ]. hsa-miR-494 (upregulated) and hsa-miR-9 (downregulated) have the maximum number of targets in proteins identified in RB tumor exosomes ( Table 1 ).…”

Section: Mirna and Their Targets In Retinoblastomamentioning

confidence: 99%

“…The proteins identified in the exosomes of RB tumor are shown to have a role in cell growth and tumorigenesis, as discussed. Proteins abl interactor 2 (ABI2), neurocan (NCAN), V-type proton ATPase subunit C1 (ATP6V1C1), and UBX domain protein 4 (UBXN4) are identified in RBT-derived exosomes [ 114 ]. Studies have shown that ABI2 (Abl-interactor 2 protein) suppresses cell growth, and its truncated form plays a role in the acceleration of tumorigenesis, suggesting that ABI2 may be a tumor suppressor in RB [ 145 , 146 ].…”

Section: Mirna and Their Targets In Retinoblastomamentioning

confidence: 99%

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Exosomes: Insights from Retinoblastoma and Other Eye Cancers

Lande

Gupta

Ranjan

et al. 2020

IJMS

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Exosomes, considered as cell debris or garbage bags, have been later characterized as nanometer-sized extracellular double-membrane lipid bilayer bio-vesicles secreted by the fusion of vesicular bodies with the plasma membrane. The constituents and the rate of exosomes formation differ in different pathophysiological conditions. Exosomes are also observed and studied in different parts of the eye, like the retina, cornea, aqueous, and vitreous humor. Tear fluid consists of exosomes that are shown to regulate various cellular processes. The role of exosomes in eye cancers, especially retinoblastoma (RB), is not well explored, although few studies point towards their presence. Retinoblastoma is an intraocular tumor that constitutes 3% of cases of cancer in children. Diagnosis of RB may require invasive procedures, which might lead to the spread of the disease to other parts. Due to this reason, better ways of diagnosis are being explored. Studies on the exosomes in RB tumors and serum might help designing better diagnostic approaches for RB. In this article, we reviewed studies on exosomes in the eye, with a special emphasis on RB. We also reviewed miRNAs expressed in RB tumor, serum, and cell lines and analyzed the targets of these miRNAs from the proteins identified in the RB tumor exosomes. hsa-miR-494 and hsa-miR-9, upregulated and downregulated, respectively in RB, have the maximum number of targets. Although oppositely regulated, they share the same targets in the proteins identified in RB tumor exosomes. Overall this review provides the up-to-date progress in the area of eye exosome research, with an emphasis on RB.

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Proteomic Profiling of Retinoblastoma-Derived Exosomes Reveals Potential Biomarkers of Vitreous Seeding

Cited by 33 publications

References 127 publications

The Emerging Role of Small Extracellular Vesicles in Inflammatory Airway Diseases

The Emerging Role of Small Extracellular Vesicles in Inflammatory Airway Diseases

Autophagy and Exosomes Relationship in Cancer: Friends or Foes?

Exosomes: Insights from Retinoblastoma and Other Eye Cancers

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