Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Pelá, Vinícius Taioqui; Lunardelli, João Guilherme Quintal; Ventura, Talita Mendes Oliveira; Camiloti, Gabriel Domingues; Baumann, Tommy; Carvalho, Thiago Saads; Lussi, Adrian; Buzalaf, Marília Afonso Rabelo

doi:10.1111/eos.12744

Cited by 13 publications

(10 citation statements)

References 28 publications

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“…Moreover, a recent study by our group compared the proteomic profile of the AEP formed in vitro, in situ, and in vivo. The AEP formed in situ was similar to in vivo (Pelá et al 2020). Considering that our preventive strategy is based on AEP engineering, the results obtained in situ with this protein might be highly comparable with in vivo conditions.…”

Section: Discussionsupporting

confidence: 64%

Safety and In Situ Antierosive Effect of CaneCPI-5 on Dental Enamel

et al. 2021

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The sugarcane cystatin (CaneCPI-5) was recently cloned and showed strong binding force to dental enamel and protection against initial erosion. However, evaluations on its safety and efficacy in a situation closer to the clinical condition are necessary. In the present study we analyzed 1) the cytotoxicity of CaneCPI-5 on human gingival fibroblasts (HGFs); 2) the ability of CaneCPI-5 to reduce enamel erosion and erosion+abrasion in situ. In part 1, HGFs were treated with CaneCPI-5 (0.025, 0.05, 0.1, 0.5 or 1.0 mg/mL) or no treatment (control). The cytotoxicity was assessed after 60 s and 24 h by mitochondrial activity (MTT), confocal microscopy, and hematoxylin/eosin staining. In part 2, 15 volunteers participated in a double-blind crossover protocol consisting of 3 phases, according to the following treatments: 1) 0.1 mg/mL CaneCPI-5; 2) SnCl2/NaF/AmF (Elmex; positive control); 3) water (negative control). The volunteers wore an appliance containing 4 bovine enamel specimens for 5 d. Each day, the specimens were individually treated with 50 µL of the tested solutions per 60 s and then subjected to erosive challenges (0.1% citric acid, pH 2.5, for 90 s, 4 times per day). After the first and last erosive challenge each day, 2 samples were abraded (toothbrushing, 15 s). Enamel wear was measured by contact profilometry. One or two-way analysis of variance (ANOVA)/Tukey’s or Sidak’s tests ( P < 0.05) were applied. Regardless of the concentration and the experimental time, CaneCPI-5 did not decrease the cell viability compared to the negative control ( P < 0.05). Erosion+abrasion led to significantly greater wear compared to erosion only. For both conditions, the lowest wear was found for SnCl2 and CaneCPI-5, which did not differ significantly from each other, but showed significant protection when compared to the negative control. In conclusion, CaneCPI-5 is safe on HGFs and reduces enamel erosive wear to the same extent as a commercial solution used to control erosive tooth wear (ETW).

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Section: Discussionsupporting

confidence: 64%

Safety and In Situ Antierosive Effect of CaneCPI-5 on Dental Enamel

et al. 2021

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“…The qualitative profile of the proteins, especially the typical ones, is different in the in vitro condition. Furthermore, there are important quantitative differences that may interfere when attempting to extrapolate in vitro results to an in situ and in vivo condition [51].…”

Section: Discussionmentioning

confidence: 99%

Proteomic profile of the acquired enamel pellicle of children with early childhood caries and caries‐free children

Oliveira

Buzalaf

Silva

et al. 2023

European J Oral Sciences

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Acquired enamel pellicle plays an important role in the pathogenesis of early childhood caries (ECC), working as a protective interface between the tooth and the oral cavity. The aim of this cross-sectional in vivo proteomic study was to compare the acquired enamel pellicle protein profile of 3-5-year-old children with ECC (n = 10) and caries-free children (n = 10). Acquired enamel pellicle samples were collected and processed for proteomic analysis (nLC-ESI-MS/MS). In total, 241 proteins were identified. Basic salivary proline-rich protein 1 and 2, Cystatin-B, and SA were found only in the caries free group. When comparing caries free and ECC groups, lower protein levels were found in the caries free group for hemoglobin subunit beta, delta, epsilon, gamma-2, globin domain-containing protein and gamma-1, neutrophil defensin 3, serum albumin, protein S100-A8, and S100-A9. The proteins histatin-1, statherin, salivary acidic proline-rich phosphoprotein ½, proline-rich protein 4, submaxillary gland androgen-regulated protein 3B, alpha-amylase 1 and 2B were found at higher levels in the caries free group. The exclusive and the proteins found at higher levels in the caries free group might have protective functions that play a role in the prevention of caries, besides providing important insights to be evaluated in future studies for the possible development of new therapeutic strategies for ECC.

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“…These results, however, should still be viewed with the caveat that this study has some limitations. Primarily, even though we used human saliva, we formed the salivary pellicle in vitro , which can be considerably different from those formed in vivo [ 44 ]. Secondly, although we used human teeth, the specimens were ground to reach a healthy (non-demineralized) layer of enamel.…”

Section: Discussionmentioning

confidence: 99%

Synergistic effect between plant extracts and fluoride to protect against enamel erosion: An in vitro study

et al. 2022

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Polyphenol-rich solutions, such as plant extracts and teas, can modify the salivary pellicle and improve the protection against dental erosion. In this study, we further explored how these polyphenol-rich plant extracts solutions behave in the presence of fluoride. We distributed enamel specimens into 9 groups (n = 15): Control_No_F- (Deionized water); Control_F- (500 ppm F-), Grape_Seed_No_F- (Grape seed extract), Grape_Seed_F- (Grape seed extract + 500 ppm F-), Grapefruit_Seed_No_F- (Grapefruit seed extract), Grapefruit_Seed_F- (Grapefruit seed extract + 500 ppm F-), Blueberry_No_F- (Blueberry extract), Blueberry_F- (Blueberry extract + 500 ppm F-), and Sn2+/F-_Rinse (commercial solution containing 800 ppm Sn2+ and 500 ppm F-). The specimens were submitted to 5 cycles (1 cycle per day), and each cycle consisted of: salivary pellicle formation (human saliva, 30 min, 37°C), modification of the pellicle (2 min, 25°C), pellicle formation (60 min, 37°C), and an erosive challenge (1 min, citric acid). Between cycles, the specimens were kept in a humid chamber. Relative surface hardness (rSH), relative surface reflection intensity (rSRI) and calcium released to the acid were analysed, using general linear models, and Kruskal-Wallis with post-hoc Dunn’s tests. We observed that the presence of fluoride in synergy with the extract solutions provided better protection than the groups containing extract or fluoride only. For rSH, we observed a significant main effect of extracts (F(4,117) = 9.20; p<0.001) and fluoride (F(1,117) = 511.55; p<0.001), with a significant interaction (F(3,117) = 6.71; p<0.001). Grape_Seed_F- showed the best protection, better than fluoride, and Sn2+/F-_Rinse. Calcium results also showed greater protection for the groups containing fluoride, whereas for rSRI, despite a significant interaction between extract and fluoride (F(3,117) = 226.05; p<0.001), the differences between the groups were not as clearly observed. We conclude that polyphenols from plant extracts, when combined with fluoride, improve the protective effect of salivary pellicles against enamel erosion.

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Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Cited by 13 publications

References 28 publications

Safety and In Situ Antierosive Effect of CaneCPI-5 on Dental Enamel

Safety and In Situ Antierosive Effect of CaneCPI-5 on Dental Enamel

Proteomic profile of the acquired enamel pellicle of children with early childhood caries and caries‐free children

Synergistic effect between plant extracts and fluoride to protect against enamel erosion: An in vitro study

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