Abstract:Polyphenol-rich solutions, such as plant extracts and teas, can modify the salivary pellicle and improve the protection against dental erosion. In this study, we further explored how these polyphenol-rich plant extracts solutions behave in the presence of fluoride. We distributed enamel specimens into 9 groups (n = 15): Control_No_F- (Deionized water); Control_F- (500 ppm F-), Grape_Seed_No_F- (Grape seed extract), Grape_Seed_F- (Grape seed extract + 500 ppm F-), Grapefruit_Seed_No_F- (Grapefruit seed extract)… Show more
“…Discrepancies in the study protocols, including variations in AEP formation and solution application times, may account for these con icting results. In our study, proanthocyanidin solutions were applied to the enamel for 30 seconds following AEP formation over thirty minutes [30,32], followed by an additional one-hour pellicle formation [31,32]. In contrast, Boteon et al [21] applied proanthocyanidin gel for 1 minute after a two-hour AEP formation period.…”
Section: Discussionmentioning
confidence: 97%
“…In Rios et al [16], the AEP was formed in situ using intraoral appliances for a two-hour period, then the specimens were treated and submitted to erosive cycling. In the present study, the AEP was formed for thirty minutes [30,32]. Subsequently, the specimens were treated, and the appliances were reinserted into the mouth of the volunteers for an additional hour-long period to modify the AEP [31,32].…”
Section: Discussionmentioning
confidence: 98%
“…As expected, the solution resulted in minor percentage of hardness loss (around 11%). The protective effect of SnF is attributed to the incorporation of uoride and stannous ions into the enamel, and also to the modi cation of the AEP [32,35,36]. Fluoride can modulate the composition of the pellicle by increasing statherin and statin concentrations [32,37].…”
Objectives
This in vitro study evaluated the effect of Proanthocyanidin, Palm Oil and Vitamin E against initial erosion.
Materials and Methods
Bovine enamel blocks (n = 140) were divided into 14 groups: PC_SnCl2/NaF/Am-F-containing solution (positive control); NC_deionized water (negative control); PO_palm oil; P6.5_6.5% proanthocyanidin; P2_2% proanthocyanidin; VE_Vitamin E; POP6.5_palm oil + 6.5% proanthocyanidin; P6.5PO_6.5% proanthocyanidin + palm oil; POP2_palm oil + 2% proanthocyanidin; P2PO_2% proanthocyanidin + palm oil; VEP6.5_Vitamin E + 6.5% proanthocyanidin; P6.5VE_6.5% proanthocyanidin + Vitamin E; VEP2_Vitamin E + 2% proanthocyanidin; P2VE_2% proanthocyanidin + Vitamin E. The acquired enamel pellicle (AEP) was previously formed in situ for 30 min. The specimens were treated in vitro with the solutions (500 µl, 30s). Then, the blocks were maintained for an additional hour in oral cavity to develop the modified AEP. The blocks were immersed in 0.5% citric acid (pH 2.5) during 30s. The response variable was the percentage of surface hardness loss. Data were analyzed by one-way ANOVA and Fisher's Test (p < 0.05).
Results
P6.5VE was the only group to promote protection similar to PC. PO, P2, POP6.5, P2PO, P6.5VE and P2VE exhibited %SHL similar to both PC and NC groups. (p < 0.05). P6.5, VE, P6.5PO, POP2, VEP6.5 and VEP2 were different to PC and similar to NC.
Conclusion
It is concluded that proanthocyanidin associated to vitamin E was able to protect enamel against in vitro initial erosive challenge.
Clinical Relevance:
This study suggests the potential for a cost-effective anti-erosion product. Further investigations across diverse experimental models are warranted to validate these promising outcomes.
“…Discrepancies in the study protocols, including variations in AEP formation and solution application times, may account for these con icting results. In our study, proanthocyanidin solutions were applied to the enamel for 30 seconds following AEP formation over thirty minutes [30,32], followed by an additional one-hour pellicle formation [31,32]. In contrast, Boteon et al [21] applied proanthocyanidin gel for 1 minute after a two-hour AEP formation period.…”
Section: Discussionmentioning
confidence: 97%
“…In Rios et al [16], the AEP was formed in situ using intraoral appliances for a two-hour period, then the specimens were treated and submitted to erosive cycling. In the present study, the AEP was formed for thirty minutes [30,32]. Subsequently, the specimens were treated, and the appliances were reinserted into the mouth of the volunteers for an additional hour-long period to modify the AEP [31,32].…”
Section: Discussionmentioning
confidence: 98%
“…As expected, the solution resulted in minor percentage of hardness loss (around 11%). The protective effect of SnF is attributed to the incorporation of uoride and stannous ions into the enamel, and also to the modi cation of the AEP [32,35,36]. Fluoride can modulate the composition of the pellicle by increasing statherin and statin concentrations [32,37].…”
Objectives
This in vitro study evaluated the effect of Proanthocyanidin, Palm Oil and Vitamin E against initial erosion.
Materials and Methods
Bovine enamel blocks (n = 140) were divided into 14 groups: PC_SnCl2/NaF/Am-F-containing solution (positive control); NC_deionized water (negative control); PO_palm oil; P6.5_6.5% proanthocyanidin; P2_2% proanthocyanidin; VE_Vitamin E; POP6.5_palm oil + 6.5% proanthocyanidin; P6.5PO_6.5% proanthocyanidin + palm oil; POP2_palm oil + 2% proanthocyanidin; P2PO_2% proanthocyanidin + palm oil; VEP6.5_Vitamin E + 6.5% proanthocyanidin; P6.5VE_6.5% proanthocyanidin + Vitamin E; VEP2_Vitamin E + 2% proanthocyanidin; P2VE_2% proanthocyanidin + Vitamin E. The acquired enamel pellicle (AEP) was previously formed in situ for 30 min. The specimens were treated in vitro with the solutions (500 µl, 30s). Then, the blocks were maintained for an additional hour in oral cavity to develop the modified AEP. The blocks were immersed in 0.5% citric acid (pH 2.5) during 30s. The response variable was the percentage of surface hardness loss. Data were analyzed by one-way ANOVA and Fisher's Test (p < 0.05).
Results
P6.5VE was the only group to promote protection similar to PC. PO, P2, POP6.5, P2PO, P6.5VE and P2VE exhibited %SHL similar to both PC and NC groups. (p < 0.05). P6.5, VE, P6.5PO, POP2, VEP6.5 and VEP2 were different to PC and similar to NC.
Conclusion
It is concluded that proanthocyanidin associated to vitamin E was able to protect enamel against in vitro initial erosive challenge.
Clinical Relevance:
This study suggests the potential for a cost-effective anti-erosion product. Further investigations across diverse experimental models are warranted to validate these promising outcomes.
“…For the total calcium release, 1 ml of citric acid after each erosive cycle was mixed per specimen and analyzed with an atomic absorption spectrometer (Aanalyst 400, Perkin-Elmer Analytical Instruments). The measured amount of calcium was normalized to the exposed dentine surface area [ 33 ]. The later was measured using a light microscope connected to a camera (Leica, M420 and Leica DFC495, respectively) under 20x magnification and a software program (IM500).…”
Objectives
To verify the protective effect of plant extracts associated with fluoride against dental erosion of dentine, in the presence and absence of a salivary pellicle.
Methods
Dentine specimens (n = 270) were randomly distributed into 9 experimental groups (n = 30/group): GT (green tea extract); BE (blueberry extract); GSE (grape seed extract); NaF (sodium fluoride); GT+NaF (green tea extract and NaF); BE+NaF (blueberry extract and NaF); GSE+NaF (grape seed extract and NaF); negative control (deionized water); and a positive control (commercialized mouthrinse containing stannous and fluoride). Each group was further divided into two subgroups (n = 15), according to the presence (P) or absence (NP) of salivary pellicle. The specimens were submitted to 10 cycles: 30 min incubation in human saliva (P) or only in humid chamber (NP), 2 min immersion in experimental solutions, 60 min of incubation in saliva (P) or not (NP), and 1 min erosive challenge. Dentine surface loss (dSL-10 and dSL-total), amount of degraded collagen (dColl) and total calcium release (CaR) were evaluated. Data were analyzed with Kruskal-Wallis, Dunn’s and Mann-Whitney U tests (p>0.05).
Results
Overall, the negative control presented the highest values of dSL, dColl and CaR, and the plant extracts showed different degrees of dentine protection. For the subgroup NP, GSE showed the best protection of the extracts, and the presence of fluoride generally further improved the protection for all extracts. For the subgroup P, only BE provided protection, while the presence of fluoride had no impact on dSL and dColl, but lowered CaR. The protection of the positive control was more evident on CaR than on dColl.
Conclusion
We can conclude that the plant extracts showed a protective effect against dentine erosion, regardless of the presence of salivary pellicle, and that the fluoride seems to improve their protection.
The fruits of Phyllanthus emblica linn., mainly grows in tropical and subtropical regions and is renowned for its medicine and food homology properties. It has a distinctive flavor, great nutritional...
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