Proteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with Roles in Plant Cell Wall Degradation and Virulence

Abstract

Rhizoctonia solani AG 4 is a soilborne necrotrophic fungal plant pathogen that causes economically important diseases on agronomic crops worldwide. This study used a proteomics approach to characterize both intracellular proteins and the secretome of R. solani AG 4 isolate Rs23A under several growth conditions, the secretome being highly important in pathogenesis. From over 500 total secretome and soluble intracellular protein spots from 2-D gels, 457 protein spots were analyzed and 318 proteins positively matched with fungal proteins of known function by comparison with available R. solani genome databases specific for anastomosis groups 1-IA, 1-IB, and 3. These proteins were categorized to possible cellular locations and functional groups and for some proteins their putative roles in plant cell wall degradation and virulence. The majority of the secreted proteins were grouped to extracellular regions and contain hydrolase activity.

Application of Proteomics for the Investigation of the Effect of Initial pH on Pathogenic Mechanisms of Fusarium proliferatum on Banana Fruit

“…Many researches have reported that CWDEs are involved in the direct degradation of plant tissue, and they have been suggested to be pathogenicity factors of several pathogens, such as pectate lyase (PL), polygalacturonase (PG), etc. (Zhang et al, 2014 ; Lakshman et al, 2016 ). In this study, alkaline environment significantly inhibited the accumulation of CWDEs (Table 2 ), including 1, 3-beta-glucanosyltransferase (SP18), SPR1-exo-1, 3-beta-glucanase (SP19), glucan 1, 3-beta-glucosidase (SP20), endo alpha-1, 4 polygalactosaminidase precusor (SP21), gluconolactonase (SP23), and probable cellulose (SP24).…”

“…It is well known that extracellular proteins related to CWDEs or proteolysis are important for the pathogenicity of plant pathogens. The plant cell wall is an internal physical defensive barrier, and pathogens use extracellular enzymes to degrade the cell wall and invade host (Lakshman et al, 2016 ). Many researches have reported that CWDEs are involved in the direct degradation of plant tissue, and they have been suggested to be pathogenicity factors of several pathogens, such as pectate lyase (PL), polygalacturonase (PG), etc.…”

“…The secreted pathogenicity factors are well known for their ability to help the pathogen successfully colonize and invade the targeted host (Zong et al, 2015 ). In recent years, proteomic approach has been widely used to explore the secretome change and infection mechanism of several fungal pathogens (Li et al, 2012 ; Meijer et al, 2014 ; Lakshman et al, 2016 ). Additionally, proteomics analyses were used to comprehensively characterize infection-specific protein expression pattern of early defense-related signaling in Medicago truncatula (Trapphoff et al, 2009 ).…”

Pangenome analysis of the soil-borne fungal phytopathogenRhizoctonia solaniand development of a comprehensive web resource: RsolaniDB

“…The rapid and relatively low-cost of generation of genomic sequences and other 'omics' datasets has played a significant role in furthering our understanding of the host-pathogen interactions and ecology of Rhizoctonia species. (8)(9)(10)(11)(12). The analysis of these genomic sequences and functional components revealed several novel or previously unrecognized classes of R. solani genes among different AGs that are .…”

Impact of Cellulose-Rich Organic Soil Amendments on Growth Dynamics and Pathogenicity of Rhizoctonia solani

“…However, the population dynamics and pathogenicity of R. solani, as affected by organic substrates, has received limited attention [29]. R. solani is a basidiomycetal fungus with the ability to produce a broad array of plant cell-walldegrading enzymes [30,31]. These are used for decomposition of cellulose, as part of both necrotrophic and saprotrophic growth of R. solani [25,32].…”