Application of Proteomics for the Investigation of the Effect of Initial pH on Pathogenic Mechanisms of Fusarium proliferatum on Banana Fruit

Li, Taotao; Wu, Qifan; Yong, Wang; John, Afiya; Qu, Hongxia; Gong, Liang; Duan, Xuewu; Zhu, Hong; Yun, Ze; Jiang, Yueming

doi:10.3389/fmicb.2017.02327

Cited by 13 publications

(11 citation statements)

References 46 publications

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“…In this study, to further mimic the natural infection environment of the fungus in planta, F. proliferatum was cultured in medium supplemented with or without banana peel (+BP/-BP). The banana peel closely represents the natural hosts of the fungus in contrast with previous studies of the F. proliferatum in vitro secretome [20,21]. This approach, combined with label-free quantitate proteomic technology, which is ideally suited for a gel-free shotgun analysis [36], can identify many fungal secreted proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Given the significant loss in the quality of fruit and potential harm caused by F. proliferatum , elucidation of this fungus infection mechanism in respect of secreted proteins is essential. Previous research investigated the effect of different media on the secretome of F. proliferatum [20,21]. These studies may not closely mimic the nutritional situation or infection environment of the fungus in planta.…”

Section: Introductionmentioning

confidence: 99%

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Secretome Profiling Reveals Virulence-Associated Proteins of Fusarium proliferatum during Interaction with Banana Fruit

Wang

et al. 2019

Biomolecules

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Secreted proteins are vital for the pathogenicity of many fungi through manipulating their hosts for efficient colonization. Fusarium proliferatum is a phytopathogenic fungus infecting many crops, vegetables, and fruit, including banana fruit. To access the proteins involved in pathogen–host interaction, we used label-free quantitative proteomics technology to comparatively analyze the secretomes of F. proliferatum cultured with and without banana peel in Czapek’s broth medium. By analyzing the secretomes of F. proliferatum, we have identified 105 proteins with 40 exclusively secreted and 65 increased in abundance in response to a banana peel. These proteins were involved in the promotion of invasion of banana fruit, and they were mainly categorized into virulence factors, cell wall degradation, metabolic process, response to stress, regulation, and another unknown biological process. The expressions of corresponding genes confirmed the existence of these secreted proteins in the banana peel. Furthermore, expression pattern suggested variable roles for these genes at different infection stages. This study expanded the current database of F. proliferatum secreted proteins which might be involved in the infection strategy of this fungus. Additionally, this study warranted the further attention of some secreted proteins that might initiate infection of F. proliferatum on banana fruit.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Secretome Profiling Reveals Virulence-Associated Proteins of Fusarium proliferatum during Interaction with Banana Fruit

Wang

et al. 2019

Biomolecules

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“…The results led to the conclusion that secretomes related to mycoparasitism and cell wall degradation are entirely different [ 294 ]. pH-dependent changes of the secretome were investigated by Li et al [ 295 ] in the fruit pathogen Fusarium proliferatum using 2D-E-MALDI-TOF/TOF-MS. A correlation was found between high pH values and the downregulation of several cell wall degrading enzymes (CWDEs). Based on these results, the role of 1,3-β-glucanosyltransferase, SPR1-exo-1,3-beta-glucanase, glucan 1,3-beta-glucosidase and gluconolactonase as pathogenicity factors was confirmed and a decrease in CWDEs was associated to a decrease in the fungus pathogenicity.…”

Section: Proteomics Advances In Ascomycotamentioning

confidence: 99%

An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota

Muggia

Ametrano

Sterflinger

et al. 2020

Life

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Fungi are among the most successful eukaryotes on Earth: they have evolved strategies to survive in the most diverse environments and stressful conditions and have been selected and exploited for multiple aims by humans. The characteristic features intrinsic of Fungi have required evolutionary changes and adaptations at deep molecular levels. Omics approaches, nowadays including genomics, metagenomics, phylogenomics, transcriptomics, metabolomics, and proteomics have enormously advanced the way to understand fungal diversity at diverse taxonomic levels, under changeable conditions and in still under-investigated environments. These approaches can be applied both on environmental communities and on individual organisms, either in nature or in axenic culture and have led the traditional morphology-based fungal systematic to increasingly implement molecular-based approaches. The advent of next-generation sequencing technologies was key to boost advances in fungal genomics and proteomics research. Much effort has also been directed towards the development of methodologies for optimal genomic DNA and protein extraction and separation. To date, the amount of proteomics investigations in Ascomycetes exceeds those carried out in any other fungal group. This is primarily due to the preponderance of their involvement in plant and animal diseases and multiple industrial applications, and therefore the need to understand the biological basis of the infectious process to develop mechanisms for biologic control, as well as to detect key proteins with roles in stress survival. Here we chose to present an overview as much comprehensive as possible of the major advances, mainly of the past decade, in the fields of genomics (including phylogenomics) and proteomics of Ascomycota, focusing particularly on those reporting on opportunistic pathogenic, extremophilic, polyextremotolerant and lichenized fungi. We also present a review of the mostly used genome sequencing technologies and methods for DNA sequence and protein analyses applied so far for fungi.

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“…Similarly, in a study (Paper et al 2007) on the same fungal species, in-planta and in-vitro analyses evidenced the presence of hydrolase, lyase, and esterase in the former, suggesting their role in pathogenesis. Likewise, another study on Fusarium proliferatum also showed inhibition of CWDEs consisting of glucanase, cellulase, and glucanosyltransferase (Li et al 2017) to be in uenced by high pH, therefore affecting its growth. Among the F. oxysporum ff spp., an in vitro comparative proteomics studies on the races of f. sp.…”

Section: Introductionmentioning

confidence: 95%

Computational proteomics analysis reveals pathogen secreted carbohydrate active enzymes in tomato xylem sap

Roy¹,

Kalita²,

Jayaprakash³

et al. 2021

Preprint

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Fusarium oxysporum f. sp. lycopersici (Fol), a causal organism of Fusarium wilt in the tomato plant, secretes cell wall degrading enzymes, also known as carbohydrate-active enzymes (CAZymes). These are crucial during colonization and pathogenesis, as evidenced by several proteomic studies, revealing the importance of these CAZymes in virulence and pathogenicity. However, few of them have been done in-planta, exhibiting differences in the expression of these cell wall degrading enzymes compared to in-vitro studies. Therefore, to explore the CAZymes involved in pathogenesis while residing in the host plant, an in-planta (xylem sap) proteomics of a susceptible tomato variety affected with Fol was done. Most of these CAZymes belonged to the hydrolase and oxidoreductase families having no significant homology with tomato proteins. Nearly 90% of them were predicted to be soluble and extracellular. The core CAZymes families with interactional evidence identified were AA3, GH3, GH18, GH20, GH28, GH43, GH47, GH55 and CE8. Thus, apart from annotating some of the hypothetical proteins to be CAZymes, the study sheds light on CAZymes families that may have a role in the pathogenesis and survival of this fungus in the susceptible tomato plant.

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Application of Proteomics for the Investigation of the Effect of Initial pH on Pathogenic Mechanisms of Fusarium proliferatum on Banana Fruit

Cited by 13 publications

References 46 publications

Secretome Profiling Reveals Virulence-Associated Proteins of Fusarium proliferatum during Interaction with Banana Fruit

Secretome Profiling Reveals Virulence-Associated Proteins of Fusarium proliferatum during Interaction with Banana Fruit

An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota

Computational proteomics analysis reveals pathogen secreted carbohydrate active enzymes in tomato xylem sap

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