Secretome Profiling Reveals Virulence-Associated Proteins of Fusarium proliferatum during Interaction with Banana Fruit

Abstract

Secreted proteins are vital for the pathogenicity of many fungi through manipulating their hosts for efficient colonization. Fusarium proliferatum is a phytopathogenic fungus infecting many crops, vegetables, and fruit, including banana fruit. To access the proteins involved in pathogen–host interaction, we used label-free quantitative proteomics technology to comparatively analyze the secretomes of F. proliferatum cultured with and without banana peel in Czapek’s broth medium. By analyzing the secretomes of F. proliferatum, we have identified 105 proteins with 40 exclusively secreted and 65 increased in abundance in response to a banana peel. These proteins were involved in the promotion of invasion of banana fruit, and they were mainly categorized into virulence factors, cell wall degradation, metabolic process, response to stress, regulation, and another unknown biological process. The expressions of corresponding genes confirmed the existence of these secreted proteins in the banana peel. Furthermore, expression pattern suggested variable roles for these genes at different infection stages. This study expanded the current database of F. proliferatum secreted proteins which might be involved in the infection strategy of this fungus. Additionally, this study warranted the further attention of some secreted proteins that might initiate infection of F. proliferatum on banana fruit.

Genome, transcriptome and secretome analyses of the antagonistic, yeast-like fungus Aureobasidium pullulans to identify potential biocontrol genes

“…Overall, and as expected, our differential secretome analysis of A. pullulans NBB 7.2.1 culture supernatants did not correlate well with the transcriptome results. This effect was expected as it had been similarly reported in other systems [47][48][49][50][51][52]. In general, protein and transcriptome levels do not seem to be highly correlated [53], which is likely due to the additional levels of regulation that determine protein levels (i.e., translation, post-translational modification, formation of complexes, degradation).…”

Pangenome analysis of the soil-borne fungal phytopathogenRhizoctonia solaniand development of a comprehensive web resource: RsolaniDB

“…To facilitate host colonization, plant pathogens secrete proteins to host compartments that modulate morphological changes in the host system and establish fungal infection (68)(69)(70).…”

“…Although these plant pathogenic fungi secrete a large number of proteins, only a small proportion of these proteins have been implicated to be effectively associated with fungal-plant interactions, i.e. effector proteins (68)(69)(70). Effector proteins can strongly inhibit the activity of host cellular proteases and allow pathogenic fungi to evade host defense mechanisms.…”

Secretome Analysis of the Banana Fusarium Wilt Fungi Foc R1 and Foc TR4 Reveals a New Effector OASTL Required for Full Pathogenicity of Foc TR4 in Banana

“…Secretome profiling and prediction have been used to identify SPs of many pathogens, including Tilletia horrida [ 10 ], Fusarium proliferatum [ 11 ], Tilletia indica [ 12 , 13 ], rose black spot [ 4 ], Trichoderma virens [ 14 ], Arbuscular mycorrhizal [ 15 ], and Puccinia striiformis f. sp. tritici [ 16 ].…”

“…tritici [ 16 ]. In the banana fruit pathogen, F. proliferatum , 105 secretory proteins were predicted based on the pathogen proteome [ 11 ]. In Foc TR4 , 2235 SPs were identified in one study, although the specific data were not shown [ 17 ].…”