Proteomic Identification of Dengue Virus Binding Proteins in<i>Aedes aegypti</i>Mosquitoes and<i>Aedes albopictus</i>Cells

Muñoz, Marı́a de Lourdes; Limón-Camacho, Gustavo; Tovar, Rosalinda; Diaz-Badillo, Alvaro; Mendoza‐Hernández, Guillermo; Black, William C.

doi:10.1155/2013/875958

Cited by 27 publications

(30 citation statements)

References 72 publications

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“…The enolase TIP identified in the present study was previously reported to be upregulated in L1 thrips infected with TSWV (18), and enolase was also upregulated in response to rice stripe virus (RSV) in bodies of the planthopper vector, L. striatellus (57). In the case of flaviviruses, Aedes aegypti enolase was shown to directly interact with purified virus and recombinant envelope glycoprotein of dengue virus (58) and West Nile virus envelope protein (59). The localization of this enolase in brush border membrane vesicles of this mosquito species (60) strengthens the case for a proposed receptor role in virus entry into vector mosquito midguts.…”

Section: Discussionsupporting

confidence: 58%

Discovery of Novel Thrips Vector Proteins That Bind to the Viral Attachment Protein of the Plant Bunyavirus Tomato Spotted Wilt Virus

Badillo-Vargas¹,

Chen

Martin

et al. 2019

J Virol

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The plant-pathogenic virus tomato spotted wilt virus (TSWV) encodes a structural glycoprotein (GN) that, like with other bunyavirus/vector interactions, serves a role in viral attachment and possibly in entry into arthropod vector host cells. It is well documented that Frankliniella occidentalis is one of nine competent thrips vectors of TSWV transmission to plant hosts. However, the insect molecules that interact with viral proteins, such as GN, during infection and dissemination in thrips vector tissues are unknown. The goals of this project were to identify TSWV-interacting proteins (TIPs) that interact directly with TSWV GN and to localize the expression of these proteins in relation to virus in thrips tissues of principal importance along the route of dissemination. We report here the identification of six TIPs from first-instar larvae (L1), the most acquisition-efficient developmental stage of the thrips vector. Sequence analyses of these TIPs revealed homology to proteins associated with the infection cycle of other vector-borne viruses. Immunolocalization of the TIPs in L1 revealed robust expression in the midgut and salivary glands of F. occidentalis, the tissues most important during virus infection, replication, and plant inoculation. The TIPs and GN interactions were validated using protein-protein interaction assays. Two of the thrips proteins, endocuticle structural glycoprotein and cyclophilin, were found to be consistent interactors with GN. These newly discovered thrips protein-GN interactions are important for a better understanding of the transmission mechanism of persistent propagative plant viruses by their vectors, as well as for developing new strategies of insect pest management and virus resistance in plants. IMPORTANCE Thrips-transmitted viruses cause devastating losses to numerous food crops worldwide. For negative-sense RNA viruses that infect plants, the arthropod serves as a host as well by supporting virus replication in specific tissues and organs of the vector. The goal of this work was to identify thrips proteins that bind directly to the viral attachment protein and thus may play a role in the infection cycle in the insect. Using the model plant bunyavirus tomato spotted wilt virus (TSWV), and the most efficient thrips vector, we identified and validated six TSWV-interacting proteins from Frankliniella occidentalis first-instar larvae. Two proteins, an endocuticle structural glycoprotein and cyclophilin, were able to interact directly with the TSWV attachment protein, GN, in insect cells. The TSWV GN-interacting proteins provide new targets for disrupting the viral disease cycle in the arthropod vector and could be putative determinants of vector competence.

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Section: Discussionsupporting

confidence: 58%

Discovery of Novel Thrips Vector Proteins That Bind to the Viral Attachment Protein of the Plant Bunyavirus Tomato Spotted Wilt Virus

Badillo-Vargas¹,

Chen

Martin

et al. 2019

J Virol

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“…Laminin receptors, on the other hand, have also been implicated in DENV entry into cells of the mosquito vector Aedes albopictus (89). In the second DENV mosquito vector Aedes aegypti, E glycoprotein affinity enrichment and MS identified enolase, beta-adrenergic receptor kinase (beta-ARK), and cadherin as putative entry factors (90). Further postulated receptors for flaviviruses identified by virus overlay protein binding assay followed by MS identification, are the 78 kDa glucose-regulated protein, mosquito 45 kDa, and 64/67 kDa proteins for DENV (91)(92)(93)(94)(95) and alpha V beta 3 integrin, the C45 like phosphatase mosPTP-1 and C-type lectin mosGCTL-1 for WNV ( Fig.…”

Section: Zikvmentioning

confidence: 99%

Protein Interactions during the Flavivirus and Hepacivirus Life Cycle

Gerold

Bruening

Weigel

et al. 2017

Molecular & Cellular Proteomics

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Protein-protein interactions govern biological functions in cells, in the extracellular milieu, and at the border between cells and extracellular space. Viruses are small intracellular parasites and thus rely on protein interactions to produce progeny inside host cells and to spread from cell to cell. Usage of host proteins by viruses can have severe consequences apoptosis, metabolic disequilibria, or altered cell proliferation and mobility. Understanding protein interactions during virus infection can thus educate us on viral infection and pathogenesis mechanisms. Moreover, it has led to important clinical translations, including the development of new therapeutic and vaccination strategies. Here, we will discuss protein interactions of members of the family, which are small enveloped RNA viruses. Dengue virus, Zika virus and hepatitis C virus belong to the most prominent human pathogenic With a genome of roughly ten kilobases encoding only ten viral proteins, display intricate mechanisms to engage the host cell machinery for their purpose. In this review, we will highlight how dengue virus, hepatitis C virus, Japanese encephalitis virus, tick-borne encephalitis virus, West Nile virus, yellow fever virus, and Zika virus proteins engage host proteins and how this knowledge helps elucidate infection. We will specifically address the protein composition of the virus particle as well as the protein interactions during virus entry, replication, particle assembly, and release from the host cell. Finally, we will give a perspective on future challenges in interaction proteomics and why we believe these challenges should be met.

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“…The results for HspA5 protein (isoform of the Hsp70 chaperone) [15] corroborates data found during blood feeding in the A. aegypti midgut, where there is an increase in the gene expression of Hsp70 in response to stress [16]. However, little is known about the function of elongation factor 4 (EF4), but it is considered essential for protein synthesis and cell viability due to its homology to EF1 [17,18], which increases its expression in the A. aegypti midgut following infection with DENV-2, suggesting that it may assist in viral replication [6].…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, when infected with CHIK virus or Dengue virus type 2 (DENV-2), modified expressions of proteins associated with regulatory, metabolic and structural pathways in the midgut were noted [5]. Moreover, midgut proteins linked to DENV-2 infection, including enolase, beta-ARK, and cadherin that can act as receptors, and the elongation factor EF-1 alpha/Tu that is important for viral replication [6], have recently been identified.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of Aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets

Fernandes

Magalhães-Júnior

Baracat‐Pereira

et al. 2016

Parasitology International

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In this work we analyzed protein expression in the Aedes aegypti midgut during the larval (fourth instar, L4), pupal, and adult stages [including newly emerged (NE), sugar-fed (SF) and blood-fed (BF) females]. Two-dimensional electrophoresis showed 13 spots in the midgut of larvae, 95 in the midgut of pupae, 90 in the midgut of NE, and 76 in the midgut of SF or BF females. In the larval midguts, high serpin expression was noted, while in the pupae, protein abundance was lower than in the NE, SF, and BF females. The spots related to proteins linked to energy production, protein metabolism, signaling, and transport were highly expressed in the NE stage, while spots related proteins involved in translation were abundant in SF and BF females. The differential abundance of proteins in the midgut of A. aegypti at different developmental stages supports the necessity for midgut development during immature stage followed by the necessity of proteins related to digestion in adults.

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Proteomic Identification of Dengue Virus Binding Proteins inAedes aegyptiMosquitoes andAedes albopictusCells

Cited by 27 publications

References 72 publications

Discovery of Novel Thrips Vector Proteins That Bind to the Viral Attachment Protein of the Plant Bunyavirus Tomato Spotted Wilt Virus

Discovery of Novel Thrips Vector Proteins That Bind to the Viral Attachment Protein of the Plant Bunyavirus Tomato Spotted Wilt Virus

Protein Interactions during the Flavivirus and Hepacivirus Life Cycle

Proteomic analysis of Aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets

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