Proteomic analysis of Aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets

Fernandes, Kenner Morais; Magalhães-Júnior, Marcos Jorge de; Baracat‐Pereira, Maria Cristina; Martins, Gustavo Ferreira

doi:10.1016/j.parint.2016.08.008

Cited by 5 publications

(3 citation statements)

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“…The understanding of the hematophagic and gonotrophic capacitation (HGC) as a maturation process that occurs in newly emerged females is fundamentally important to conceptually identify specific new targets for vector control. Previous transcriptomic, microarray and proteomic studies have studied the post-emergence (PE) phase in females, revealing altered metabolic pathways in midgut, fat body and salivary glands [16][17][18][19][20]74], but just the mosquito antenae transcriptome compared gene expression based both on sex and time [21]. Notwithstanding, the available knowledge did not consider the sexual dimorphism in the time context of PE phase and were mostly focused on the fat body.…”

Section: Discussionmentioning

confidence: 99%

Aedes aegypti post-emergence transcriptome: Unveiling the molecular basis for the hematophagic and gonotrophic capacitation

et al. 2021

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The adult females of Aedes aegypti mosquitoes are facultative hematophagous insects but they are unable to feed on blood right after pupae emergence. The maturation process that takes place during the first post-emergence days, hereafter named hematophagic and gonotrophic capacitation, comprises a set of molecular and physiological changes that prepare the females for the first gonotrophic cycle. Notwithstanding, the molecular bases underlying mosquito hematophagic and gonotrophic capacitation remain obscure. Here, we investigated the molecular and biochemical changes in adult Ae. aegypti along the first four days post-emergence, prior to a blood meal. We performed a RNA-Seq analysis of the head and body, comparing male and female gene expression time courses. A total of 811 and 203 genes were differentially expressed, respectively in the body and head, and both body parts showed early, mid, and late female-specific expression profiles. Female-specific up-regulation of genes involved in muscle development and the oxidative phosphorylation pathway were remarkable features observed in the head. Functional assessment of mitochondrial oxygen consumption in heads showed a gradual increase in respiratory capacity and ATP-linked respiration as a consequence of induced mitochondrial biogenesis and content over time. This pattern strongly suggests that boosting oxidative phosphorylation in heads is a required step towards blood sucking habit. Several salivary gland genes, proteases, and genes involved in DNA replication and repair, ribosome biogenesis, and juvenile hormone signaling were up-regulated specifically in the female body, which may reflect the gonotrophic capacitation. This comprehensive description of molecular and biochemical mechanisms of the hematophagic and gonotrophic capacitation in mosquitoes unravels potentially new targets for vector control.

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Section: Discussionmentioning

confidence: 99%

Aedes aegypti post-emergence transcriptome: Unveiling the molecular basis for the hematophagic and gonotrophic capacitation

et al. 2021

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“…However, most of the proteins up/downregulated were found in the 11-30 kDa range approximately. In this molecular weight range, odorant-binding proteins, transport, signaling, and regulation proteins had been reported, this suggests that the separated proteins in this range are implied in the repellent reception, and transport processes [51][52][53] www.nature.com/scientificreports/ Subsequently, for both repellent kinds, through MASCOT, Swissprot, and Uniprot databases, proteins were identified as well as their function. The results displayed peptides similarly with a score confidence medium through high , linked to proteins from bacteria, Drosophila melanogaster, and Anopheles gambiae.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of action mechanisms between natural and synthetic repellents through neuronal electroantennogram and proteomic in Aedes aegypti (Diptera: Culicidae)

Pulido

Duitama

Velasquez-Martinez

et al. 2022

Sci Rep

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Natural-based compounds with repellent activity arise nowadays with the possibility to replace commercial synthetic repellents wholly or partially, such as N,N-Diethyl-m-toluamide (DEET). It is due to DEET's demonstrated toxicity and cutaneous irritation for human beings. Besides, research recommends avoiding using it with kids and pregnant women. The search for a repellent product implies early stages of detailed research that resolve the modes of action against the target insect. Therefore the objective of the current study was to analyze neuronal electrophysiological signals and olfactory system protein expression when the Aedes aegypti mosquito with exposition to natural-based repellents. Adult females of Ae. aegypti of Rockefeller strain were exposed to specific concentrations of repellent compounds like geranyl acetate, α-bisabolol, nerolidol, and DEET. The neuronal effect was measured by electroantennography technique, and the effect of exposure to either DEET or a mixture of natural molecules on protein expression was determined with 2D-PAGE followed by MALDI-TOF-mass spectrometry (MS). This approach revealed that DEET affected proteins related to synapses and ATP production, whereas natural-based repellents increased transport, signaling, and detoxification proteins. The proteomic and electrophysiology experiments demonstrated that repellent exposure disrupts ionic channel activity and modifies neuronal synapse and energy production processes.

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“…aegypti. Previous proteomics reports regarding this mosquito species focused on its subproteomes, including those for the midgut [2,25], salivary gland [6,[26][27][28], accessory gland, and seminal fluid [29,30]. Hence, this study aimed to supplement the available Ae.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Total Female and Male Aedes aegypti Proteomes Reveals Significant Predictive Protein–Protein Interactions, Functional Ontologies, and Differentially Abundant Proteins

Shettima

Joseph

Ishak

et al. 2021

Insects

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Aedes aegypti is a significant vector for many tropical and subtropical flavivirus diseases. Only the female mosquito transmits pathogens, while the male plays a vital role in mating and species continuity. This study explored the total proteomes of females and males based on the physiological and genetic differences of female and male mosquitoes. Protein extracts from mosquitoes were analysed using LC–ESI–MS/MS for protein identification, protein interaction network analysis, functional ontology enrichment, and differential protein abundance analyses. Protein identification revealed 422 and 682 proteins exclusive to males and females, respectively, with 608 common proteins found in both sexes. The most significant PPIs (<1.0 × 10−16) were for common proteins, followed by proteins exclusive to females (<1.0 × 10−16) and males (1.58 × 10−12). Significant functional enrichments were observed in the biological process, molecular function, and cellular component for the male and female proteins. The abundance of the proteins differed, with one protein showing an increase (elongation factor 1 α, EF1α) and two showing reductions (actin family) in females versus males. Overall, the study verified the total proteomes differences between male and female Ae. aegypti based on protein identification and interactions, functional ontologies, and differentially abundant proteins. Some of the identified proteins merit further investigation to elucidate their roles in blocking viral transmission.

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Proteomic analysis of Aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets

Cited by 5 publications

References 48 publications

Aedes aegypti post-emergence transcriptome: Unveiling the molecular basis for the hematophagic and gonotrophic capacitation

Aedes aegypti post-emergence transcriptome: Unveiling the molecular basis for the hematophagic and gonotrophic capacitation

Differentiation of action mechanisms between natural and synthetic repellents through neuronal electroantennogram and proteomic in Aedes aegypti (Diptera: Culicidae)

Evaluation of Total Female and Male Aedes aegypti Proteomes Reveals Significant Predictive Protein–Protein Interactions, Functional Ontologies, and Differentially Abundant Proteins

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