2010
DOI: 10.1590/s1678-91992010000400014
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Proteomic characterization of the thermostable toxins from Naja naja venom

Abstract: Naja naja snake venom presents abundant thermostable peptides. Many of them possess useful pharmacological activity that may be employed for drug development. For the proteomic characterization of such toxins, in the present study, Naja naja venom solution was heated up to 100°C for 10, 30, 60, 120, 180 and 300 minutes and protein fractions of non-heated and heated venom were analyzed by twodimensional nano-liquid chromatography coupled online with tandem mass spectrometry. After heating for 300 minutes, a tot… Show more

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Cited by 9 publications
(4 citation statements)
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“…Thus, similarly to the BCA method, the content of these amino acids in proteins will influence the results obtained. The Bradford assay has also been used many times to measure protein concentration in venom [8,29,[42][43][44][45]. Interestingly, this is the only method that indicates that the concentration of protein in A. contortrix venom is higher than in N. ashei and this result seems unlikely from our experience.…”
Section: Discussionmentioning
confidence: 90%
“…Thus, similarly to the BCA method, the content of these amino acids in proteins will influence the results obtained. The Bradford assay has also been used many times to measure protein concentration in venom [8,29,[42][43][44][45]. Interestingly, this is the only method that indicates that the concentration of protein in A. contortrix venom is higher than in N. ashei and this result seems unlikely from our experience.…”
Section: Discussionmentioning
confidence: 90%
“…In-gel digestion of proteins isolated by gel electrophoresis was carried out according to the protocol published by Shevchenko et al [25] with some modifications described in our previous study [3,28,29]. All chemicals including DTT, iodoacetamide (IAA), ammonium bicarbonate, ammonium acetate, trypsin (proteomics sequencing grade), sodium bicarbonate and Triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO, USA) prepared using deionized filter-sterilised MilliQ water.…”
Section: In-gel Digestionmentioning
confidence: 99%
“…The latter are so named because they share a similar protein fold with three beta‐stranded loops extending from a central core containing several conserved disulfide bonds. A number of different TFTx have been identified in cobra venom, 5 some acting as cardiotoxins, some as cytotoxins, and others as neurotoxins. In 2013 Bailly‐Chouriberry et al described a method to detect a TFTx, α‐cobratoxin, in equine plasma 6 .…”
Section: Introductionmentioning
confidence: 99%