Proteomic characterization of MPK4 signaling network and putative substrates

Zhang, Tong; Chhajed, Shweta; Schneider, Jacqueline D.; Feng, Guanying; Song, Wenlei; Chen, Sixue

doi:10.1007/s11103-019-00908-9

Cited by 11 publications

(8 citation statements)

References 69 publications

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“…The ability to test gene functions through reverse genetics in calcitrant native system is a breakthrough. Many studies have used heterologous systems-e.g., A. thaliana and Nicotiana benthamiana (N. benthamiana)-for testing gene functions [36][37][38]. While het- Based on the identified proteins from OE and WT leaves, differentially expressed proteins (DEPs) were analyzed.…”

Section: Discussionmentioning

confidence: 99%

Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum

Zhang

Tan

Zhu

et al. 2021

IJMS

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Mesembryanthemum crystallinum (common ice plant) is a halophyte species that has adapted to extreme conditions. In this study, we cloned a McHB7 transcription factor gene from the ice plant. The expression of McHB7 was significantly induced by 500 mM NaCl and it reached the peak under salt treatment for 7 days. The McHB7 protein was targeted to the nucleus. McHB7-overexpressing in ice plant leaves through Agrobacterium-mediated transformation led to 25 times more McHB7 transcripts than the non-transformed wild type (WT). After 500 mM NaCl treatment for 7 days, the activities of superoxide dismutase (SOD) and peroxidase (POD) and water content of the transgenic plants were higher than the WT, while malondialdehyde (MDA) was decreased in the transgenic plants. A total of 1082 and 1072 proteins were profiled by proteomics under control and salt treatment, respectively, with 22 and 11 proteins uniquely identified under control and salt stress, respectively. Among the 11 proteins, 7 were increased and 4 were decreased after salt treatment. Most of the proteins whose expression increased in the McHB7 overexpression (OE) ice plants under high salinity were involved in transport regulation, catalytic activities, biosynthesis of secondary metabolites, and response to stimulus. The results demonstrate that the McHB7 transcription factor plays a positive role in improving plant salt tolerance.

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Section: Discussionmentioning

confidence: 99%

Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum

Zhang

Tan

Zhu

et al. 2021

IJMS

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“…Our results showed that WRKY33 played a minor role in CA-StMPK7-triggered cell death (Figure 6d), which may be due to the fact that this transcription factor is probably not the key signalling component downstream of StMPK7 in regulating host immunity. WRKY33 was not accountable for the MPK4-regulated hormone-responsive gene expression (Qiu et al, 2008), and AtMPK4 has multiple putative substrates besides WRKY33 (Zhang, Chhajed, et al, 2019;. Furthermore, there is a plethora of WRKY genes in plants, and the minor involvement of WRKY33 in CA-StMPK7-triggered cell death may be due to redundancy of the various WRKYs.…”

Section: Discussionmentioning

confidence: 99%

Potato StMPK7 is a downstream component of StMKK1 and promotes resistance to the oomycete pathogen Phytophthora infestans

Zhang

et al. 2021

Molecular Plant Pathology

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“…Small heterogenous ribonucleoproteins (hnRNPs) with RNA-Recognition motifs (RRMs) such as HLP1, NTF2 and RNA-binding proteins (RBPs) GRP7 and GRP8 have activities that influence AS or closely connected events [ 48 , 49 ]. PABP4/8 are MPK4 substrates with likely influences on AS suppressions upon PTI [ 73 , 74 ]. Changes in GRP7 expression affects its own and GRP8 AS events resulting in NMD-mediated targeting of the splice variants [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

HopA1 Effector from Pseudomonas syringae pv syringae Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity

Dahale

Ghosh

Ingole

et al. 2021

IJMS

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Pseudomonas syringae-secreted HopA1 effectors are important determinants in host range expansion and increased pathogenicity. Their recent acquisitions via horizontal gene transfer in several non-pathogenic Pseudomonas strains worldwide have caused alarming increase in their virulence capabilities. In Arabidopsis thaliana, RESISTANCE TO PSEUDOMONAS SYRINGAE 6 (RPS6) gene confers effector-triggered immunity (ETI) against HopA1pss derived from P. syringae pv. syringae strain 61. Surprisingly, a closely related HopA1pst from the tomato pathovar evades immune detection. These responsive differences in planta between the two HopA1s represents a unique system to study pathogen adaptation skills and host-jumps. However, molecular understanding of HopA1′s contribution to overall virulence remain undeciphered. Here, we show that immune-suppressive functions of HopA1pst are more potent than HopA1pss. In the resistance-compromised ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) null-mutant, transcriptomic changes associated with HopA1pss-elicited ETI are still induced and carry resemblance to PAMP-triggered immunity (PTI) signatures. Enrichment of HopA1pss interactome identifies proteins with regulatory roles in post-transcriptional and translational processes. With our demonstration here that both HopA1 suppress reporter-gene translations in vitro imply that the above effector-associations with plant target carry inhibitory consequences. Overall, with our results here we unravel possible virulence role(s) of HopA1 in suppressing PTI and provide newer insights into its detection in resistant plants.

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Proteomic characterization of MPK4 signaling network and putative substrates

Cited by 11 publications

References 69 publications

Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum

Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum

Potato StMPK7 is a downstream component of StMKK1 and promotes resistance to the oomycete pathogen Phytophthora infestans

HopA1 Effector from Pseudomonas syringae pv syringae Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity

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