Potato StMPK7 is a downstream component of StMKK1 and promotes resistance to the oomycete pathogen <i>Phytophthora infestans</i>

Zhang, Houxiao; Li, Fangfang; Li, Zhenzhen; Cheng, Jing-Jy; Chen, Xiaokang; Wang, Qinhu; Joosten, M.H.A.J.; Shan, Weixing; Du, Yuguang

doi:10.1111/mpp.13050

Cited by 18 publications

(25 citation statements)

References 50 publications

(56 reference statements)

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“…Among them, two of the five RNA-binding proteins ( Table S1 , see online supplementary material), StUBA2a/b and StUBA2c [ 30 ], are reported to trigger SA-related plant cell death (CD) in tobacco leaves, which is also observed in CA-StMPK7 expressing N . benthamiana leaves [ 35 ]. We thus selected StUBA2a/b as a potential target of StMPK7 ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Our previous study showed that StMPK7 promotes plant immunity through SA-related immune signalling [ 35 ]. We thus determine whether UBA2a/b-mediated plant immunity depends on the SA signalling pathway.…”

Section: Resultsmentioning

confidence: 99%

“…3c , our results showed that transient expression of StUBA2a/b (StUBA2a/b-GFP) also induced cell death at 7 days post-agro-infiltration (dpi) in N. benthamiana . Our previous study showed that CA-StMPK7 induced SGT1 (Suppressor of the G2 allele of Skp1)-dependent cell death [ 35 ], we thus examined whether the cell death induced by StUBA2a/b is dependent on SGT1. By transient expressing StUBA2a/b-GFP in TRV- NbSGT1 and TRV- GUS plants, we observed that StUBA2a/b-induced cell death is abolished in NbSGT1 -silenced plants ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Further investigation reveals that StMKK1 negatively regulates potato immunity to biotrophic and hemi-biotrophic pathogens by repressing PTI and SA-related signaling pathways [ 34 ]. The StMKK1 downstream signalling target StMPK7 was found to positively regulate plant defence to Phytophthora pathogens via the SA signalling pathway [ 35 ]. However, how StMPK7 participates in plant SA-related immunity remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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StMPK7 phosphorylates and stabilizes a potato RNA-binding protein StUBA2a/b to enhance plant defence responses

Zhang

et al. 2022

Horticulture Research

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Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in regulating plant immunity. MAPKs usually transduce signals and regulate plant immunity by phosphorylating the downstream defence-related components. Our previous study indicates that StMPK7 positively regulates plant defence to Phytophthora pathogens via SA signalling pathway. However, the downstream component of StMPK7 remains unknown. In this study, we employed GFP-StMPK7 transgenic potato and performed immunoprecipitation-mass spectrometry (IP-MS) to identify the downstream component of StMPK7. We found that an RNA binding protein StUBA2a/b interacted with StMPK7, as revealed by luciferase complementation imaging (LCI) and coimmunoprecipitation (co-IP) assays. Transient expression of StUBA2a/b in N. benthamiana enhanced plant resistance to Phytophthora pathogens, while silencing of UBA2a/b decreased the resistance, suggesting a positive regulator role of UBA2a/b in plant immunity. Similar to StMPK7, StUBA2a/b was also involved in SA signalling pathway and induced SGT1-dependent cell death as constitutively activated (CA)-StMPK7 did. Immune blotting indicated that StMPK7 phosphorylates StUBA2a/b at thr248 and thr408 (T248/408) sites and stabilizes StUBA2a/b. Silencing of MPK7 in N. benthamiana suppressed StUBA2a/b-induced cell death, while co-expression with StMPK7 enhanced the cell death. Besides, StUBA2a/bT248/408A mutant showed decreased ability to trigger cell death and elevate the expression of PR genes, indicating the phosphorylation by StMPK7 enhances the functions of StUBA2a/b. Moreover, CA-StMPK7-induced cell death was largely suppressed by silencing of NbUBA2a/b, genetically implying UBA2a/b acts as the downstream component of StMPK7. Collectively, our results reveal that StMPK7 phosphorylates and stabilizes its downstream substrate StUBA2a/b to enhance plant immunity via the SA signalling pathway.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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StMPK7 phosphorylates and stabilizes a potato RNA-binding protein StUBA2a/b to enhance plant defence responses

Zhang

et al. 2022

Horticulture Research

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“…It was reported that SA treatment increased the TaMAPK4 transcripts in wheat under an avirulent race of pathogen attack, whereas knockdown the TaMAPK4 gene downregulated the SA accumulation [ 219 ]. Meanwhile, StMKK1 protein negatively regulated SA-related signalling pathways in defence against pathogens in potato [ 220 ]. Mpk4 mutant accumulated excessive levels of SA, but this was not the reason for its extreme dwarf phenotype, as knocking down the ICS1 gene (SA synthesis) did not revert mpk4-impaired growth [ 221 ].…”

Section: Possible Mechanisms Of Sa In Mitigating Abiotic Stressesmentioning

confidence: 99%

Salicylic Acid, a Multifaceted Hormone, Combats Abiotic Stresses in Plants

Liu

Qiu

Liu

et al. 2022

Life

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In recent decades, many new and exciting findings have paved the way to the better understanding of plant responses in various environmental changes. Some major areas are focused on role of phytohormone during abiotic stresses. Salicylic acid (SA) is one such plant hormone that has been implicated in processes not limited to plant growth, development, and responses to environmental stress. This review summarizes the various roles and functions of SA in mitigating abiotic stresses to plants, including heating, chilling, salinity, metal toxicity, drought, ultraviolet radiation, etc. Consistent with its critical roles in plant abiotic tolerance, this review identifies the gaps in the literature with regard to the complex signalling network between SA and reactive oxygen species, ABA, Ca2+, and nitric oxide. Furthermore, the molecular mechanisms underlying signalling networks that control development and stress responses in plants and underscore prospects for future research on SA concerning abiotic-stressed plants are also discussed.

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Ultralong‐lasting plasma‐activated water inhibits pathogens and improves plant disease resistance in soft rot‐infected hydroponic lettuce

Ran,

Zhou,

Dong

et al. 2024

Plasma Processes & Polymers

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To address the short lifetime of plasma‐activated water (PAW), this work reports on the application of ultralong‐lasting PAW for plant disease treatment. Compared with lettuce without ultralong‐lasting PAW treatment, the treated lettuce solution showed c. 1 log10 colony forming units mL−1 decrease in pathogens, as well as improvements in leaf area, leaf weight, root length, and root weight by 75%, 20%, 108.33%, and 150%, respectively. The leaf withering/root mortality rate of PAW‐treated plants was significantly lower than those of non‐PAW‐treated plants. Besides, disease defense‐related enzymes, such as catalase and β−1,3‐glucanase, were respectively enhanced by 112.5% and 5.13% in PAW‐treated lettuce, while the plant defense‐related genes were upregulated to respond to the pathogen's invasion.

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Potato StMPK7 is a downstream component of StMKK1 and promotes resistance to the oomycete pathogen Phytophthora infestans

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 18 publications

References 50 publications

StMPK7 phosphorylates and stabilizes a potato RNA-binding protein StUBA2a/b to enhance plant defence responses

StMPK7 phosphorylates and stabilizes a potato RNA-binding protein StUBA2a/b to enhance plant defence responses

Salicylic Acid, a Multifaceted Hormone, Combats Abiotic Stresses in Plants

Ultralong‐lasting plasma‐activated water inhibits pathogens and improves plant disease resistance in soft rot‐infected hydroponic lettuce

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