HopA1 Effector from Pseudomonas syringae pv syringae Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity

Dahale, Shraddha K.; Ghosh, Daipayan; Ingole, Kishor D.; Chugani, Anup; Kim, Sang Hee; Bhattacharjee, Saikat

doi:10.3390/ijms22147440

Cited by 8 publications

(4 citation statements)

References 97 publications

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“… HopA1 KP808_06050 Pseudomonas syringae pv. Lachrymans (MAFF301315) 281 99.6 Chloroplast Pyruvate dehydrogenase-dihydrolipoyl acetyltransferase Required for effector-triggered immunity [ 24 , 25 ]. HopAH1 KP808_06060 Pseudomonas amygdali pv.…”

Section: Resultsmentioning

confidence: 99%

Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1

et al. 2022

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Background Pseudomonas savastanoi is an important plant pathogen that infects and causes symptoms in a variety of economically important crops, causing considerable loss of yield and quality. Because there has been no research reported to date on bacterial canker of kiwifruit (Actinidia chinensis) plants caused by P. savastanoi and, in particular, no in-depth studies of the complete genome sequence or pathogenic mechanism, long-lasting and environmentally friendly control measures against this pathogen in kiwifruit are lacking. This study therefore has both theoretical value and practical significance. Results We report the complete genome sequence of P. savastanoi strain MHT1, which was first reported as the pathogen causing bacterial canker in kiwifruit plants. The genome consists of a 6.00-Mb chromosome with 58.5% GC content and 5008 predicted genes. Comparative genome analysis of four sequenced genomes of representative P. savastanoi strains revealed that 230 genes are unique to the MHT1 strain and that these genes are enriched in antibiotic metabolic processes and metabolic pathways, which may be associated with the drug resistance and host range observed in this strain. MHT1 showed high syntenic relationships with different P. savastanoi strains. Furthermore, MHT1 has eight conserved effectors that are highly homologous to effectors from P. syringae, Pseudomonas amygdali, and Ralstonia solanacearum strains. The MHT1 genome contains six genomic islands and two prophage sequences. In addition, 380 genes were annotated as antibiotic resistance genes and another 734 as encoding carbohydrate-active enzymes. Conclusion The whole-genome sequence of this kiwifruit bacterial canker pathogen extends our knowledge of the P. savastanoi genome, sets the stage for further studies of the interaction between kiwifruit and P. savastanoi, and provides an important theoretical foundation for the prevention and control of bacterial canker.

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Section: Resultsmentioning

confidence: 99%

Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1

et al. 2022

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“…In resistant plants, the resistasome senses HopA1 and activates RPS6 (RPS6*) through unknown mechanisms. PTI is amplified by elicited ETI HopA1 pss via MAPK3/6 signaling and other unknown mechanisms . A final interesting way in which the different signaling pathways in rice are mediated is that there are two WRKY IIa genes, OsWRKY62 and OsWRKY76, that undergo constitutive and inducible AS.…”

Section: Alternative Splicing In Plant–bacteria Interactions: Lessons...mentioning

confidence: 99%

“…PTI is amplified by elicited ETI HopA1 pss via MAPK3/6 signaling and other unknown mechanisms. 56 A final 57 In each of these models, the host increases the expression of certain isoforms via AS to better target or guide the immune system's response to harmful bacterial infection. In regard to cancer treatment, drugs have been developed to target certain subtypes based on these models.…”

Section: Function Of Alternative Splicing In Bacteria-caused Animal D...mentioning

confidence: 99%

Alternative Splicing, An Overlooked Defense Frontier of Plants with Respect to Bacterial Infection

Xie,

Zhou,

Jia

et al. 2023

J. Agric. Food Chem.

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Disease represents a major problem in sustainable agricultural development. Plants interact closely with various microorganisms during their development and in response to the prevailing environment. In particular, pathogenic microorganisms can cause plant diseases, affecting the fertility, yield, and longevity of plants. During the long coevolution of plants and their pathogens, plants have evolved both molecular pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) signaling networks in order to regulate host cells in response to pathogen infestation. Additionally, in the postgenomic era, alternative splicing (AS) has become uncovered as one of the major drivers of proteome diversity, and abnormal RNA splicing is closely associated with bacterial infections. Currently, the complexity of host−bacteria interactions is a much studied area of research that has shown steady progress over the past decade. Although the development of high-throughput sequencing technologies and their application in transcriptomes have revolutionized our understanding of AS, many mechanisms related to host−bacteria interactions remain still unclear. To this end, this review summarizes the changes observed in AS during host−bacteria interactions and outlines potential therapeutics for bacterial diseases based on existing studies. In doing so, we hope to provide guidelines for plant disease management in agriculture.

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“…Previous bioinformatics studies demonstrated sequence similarity between CaoY and HopA1 (PF17914), 3,7 a Pseudomonas syringae effector protein that suppresses the effector-triggered immunity response in plants by targeting the positive immune regulator enhanced disease susceptibility1 (EDS1). 15,16 An in vivo kinase-effector interaction screen also identified a number of other kinases as putative HopA1 targets, many of which are shared with other P. syringae effectors, such as HopAI1, 17 implying a similar S3. protein−protein interaction mechanism adopted by these two effectors.…”

Section: ■ Introductionmentioning

confidence: 99%

Mechanistic Studies on Dehydration in Class V Lanthipeptides

et al. 2022

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Lanthipeptides are ribosomally synthesized and post-translationally modified peptides characterized by lanthionine (Lan) and/or methyllanthionine (MeLan) residues. Four classes of enzymes have been identified to install these structures in a substrate peptide. Recently, a novel class of lanthipeptides was discovered that lack genes for known class I–IV lanthionine synthases in their biosynthetic gene cluster (BGC). In this study, the dehydration of Ser/Thr during the biosynthesis of the class V lanthipeptide cacaoidin was reconstituted in vitro . The aminoglycoside phosphotransferase-like enzyme CaoK iteratively phosphorylates Ser/Thr residues on the precursor peptide CaoA, followed by phosphate elimination catalyzed by the HopA1 effector-like protein CaoY to achieve eight successive dehydrations. CaoY shows sequence similarity to the OspF family proteins and the lyase domains of class III/IV lanthionine synthetases, and mutagenesis studies identified residues that are critical for catalysis. An AlphaFold prediction of the structure of the dehydration enzyme complex engaged with its substrate suggests the importance of hydrophobic interactions between the CaoA leader peptide and CaoK in enzyme–substrate recognition. This model is supported by site-directed mutagenesis studies.

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HopA1 Effector from Pseudomonas syringae pv syringae Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity

Cited by 8 publications

References 97 publications

Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1

Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1

Alternative Splicing, An Overlooked Defense Frontier of Plants with Respect to Bacterial Infection

Mechanistic Studies on Dehydration in Class V Lanthipeptides

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