Proteomic analysis of therapeutic effects of Qingyi pellet on rodent severe acute pancreatitis-associated lung injury

Sun, Zhongwei; Li, Lei; Qu, Jialin; Li, Hailong; Chen, Hailong

doi:10.1016/j.biopha.2019.109300

Cited by 13 publications

(10 citation statements)

References 64 publications

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“…Qingyi decoction (QYD), a traditional Chinese medicine (TCM) commonly used for treating SAP in the clinic, consists of eight herbs, including Rhubarb , Radix Bupleuri , Radix Aucklandiae , Paeoniae Radix Alba , Natrii Sulfas , Rhizome Corydalis , Gardenia jasminoides , and Scutellaria baicalensis Georgi ( 23 ). QYD has many pharmacological activities, including a role as an antiinflammatory, antioxidant, immune regulatory effector, a player in intestinal barrier recovery, and a promoter of cell apoptosis ( 24 – 26 ). It has been shown that QYD alleviates the systemic inflammatory responses by inhibiting the activation of NLRP3 inflammasomes in macrophages and regulating the expression of secreted phospholipase A2 and G-protein-coupled bile acid receptors in SAP ( 25 , 27 ).…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Qingyi Decoction in Severe Acute Pancreatitis-Associated Acute Lung Injury via Gut Microbiota: Targeting the Short-Chain Fatty Acids-Mediated AMPK/NF-κB/NLRP3 Pathway

Wang

Liu

et al. 2023

Microbiol Spectr

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Section: Introductionmentioning

confidence: 99%

Mechanisms of Qingyi Decoction in Severe Acute Pancreatitis-Associated Acute Lung Injury via Gut Microbiota: Targeting the Short-Chain Fatty Acids-Mediated AMPK/NF-κB/NLRP3 Pathway

Wang

Liu

et al. 2023

Microbiol Spectr

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“…Fold change (FC) > 1.2 or FC < 0.83 with P value < 0.05 was used as the primary criterion for differentially expressed proteins (DEPs) [ 14 ]. Converting DEPs' names to gene names using the UniProt database.…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of QYD against SAP-Associated ALI. Previous proteomic data were used to preliminarily screen possible targets for QYD intervention in SAP-associated ALI [14]. As shown in Figures 2(a) and 2(b), 731 differential expression proteins (DEPs) occurred between the SAP and CON groups based on the screening criteria stated in Materials and Methods, while 382 DEPs existed between the QYD and SAP groups.…”

Section: Target and Mechanismmentioning

confidence: 99%

Ferroptosis in Rat Lung Tissue during Severe Acute Pancreatitis-Associated Acute Lung Injury: Protection of Qingyi Decoction

Luo

Yang

et al. 2023

Oxidative Medicine and Cellular Longevity

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Qingyi decoction (QYD) has anti-inflammatory pharmacological properties and substantial therapeutic benefits on severe acute pancreatitis (SAP) in clinical practice. However, its protective mechanism against SAP-associated acute lung injury (ALI) remains unclear. In this study, we screened the active ingredients of QYD from the perspective of network pharmacology to identify its core targets and signaling pathways against SAP-associated ALI. Rescue experiments were used to determine the relationship between QYD and ferroptosis. Then, metabolomics and 16s rDNA sequencing were used to identify differential metabolites and microbes in lung tissue. Correlation analysis was utilized to explore the relationship between core targets, signaling pathways, metabolic phenotypes, and microbial flora, sorting out the potential molecular network of QYD against SAP-associated lung ALI. Inflammatory damage was caused by SAP in the rat lung. QYD could effectively alleviate lung injury, improve respiratory function, and significantly reduce serum inflammatory factor levels in SAP rats. Network pharmacology and molecular docking identified three key targets: ALDH2, AnxA1, and ICAM-1. Mechanistically, QYD may inhibit ferroptosis by promoting the ALDH2 expression and suppress neutrophil infiltration by blocking the cleavage of intact AnxA1 and downregulating ICAM-1 expression. Ferroptosis activator counteracts the pulmonary protective effect of QYD in SAP rats. In addition, seven significant differential metabolites were identified in lung tissues. QYD relatively improved the lung microbiome’s abundance in SAP rats. Further correlation analysis determined the correlation between ferroptosis, differential metabolites, and differential microbes. In this work, the network pharmacology, metabolomics, and 16s rDNA sequencing were integrated to uncover the mechanism of QYD against SAP-associated ALI. This novel integrated method may play an important role in future research on traditional Chinese medicine.

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“…(5) malignant tumors; (6) pregnancy; (7) time from onset of abdominal pain to hospital admission ≥48 h; and (8) unavailable medical records. Healthy volunteers undergoing medical interview and physical examination were enrolled as the control group.…”

Section: Sample Collectionmentioning

confidence: 99%

“…They found that the serum proteomic profiles well-distinguished MAP from SAP. Sun et al [7] used a mass spectrometry-based proteomic method to identify proteins before and after Qingyi pellet treatment in a rat model of SAP with lung injury. Their group found that Qingyi pellet treatment may ameliorate SAP with lung injury via cumulative or synergistic interactions of multiple components.…”

Section: Introductionmentioning

confidence: 99%

DIA-Based Proteomic Analysis of Plasma Protein Profiles in Patients with Severe Acute Pancreatitis

Zhou

et al. 2022

Molecules

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Acute pancreatitis (AP) is a pancreatic inflammatory disease that varies greatly in course and severity. To further the understanding of the pathology of AP, we carried out data-independent acquisition-based proteomic analyses using proteins extracted from the plasma of patients with severe acute pancreatitis (SAP) (experimental group) and healthy volunteers (control group). Compared to the control group, there were 35 differentially expressed proteins (DEPs) in the plasma of patients with SAP. Of those, the expression levels for 6 proteins were significantly increased, and 29 proteins were significantly decreased. Moreover, six candidate biomarkers—VWF, ORM2, CD5L, CAT, IGLV3-10, and LTF—were matched as candidate biomarkers of the disease severity of AP. The area under the receiver operating characteristic of 0.903 (95% CI: 0.839, 0.967) indicated that this combination of these six candidate biomarkers had a good prediction accuracy for predicting the severity of AP. Our study provides specific DEPs that may be useful in the diagnosis and prognosis of SAP, which suggests new theoretical bases for the occurrence and development of SAP and offers potential novel treatment strategies for SAP.

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Proteomic analysis of therapeutic effects of Qingyi pellet on rodent severe acute pancreatitis-associated lung injury

Cited by 13 publications

References 64 publications

Mechanisms of Qingyi Decoction in Severe Acute Pancreatitis-Associated Acute Lung Injury via Gut Microbiota: Targeting the Short-Chain Fatty Acids-Mediated AMPK/NF-κB/NLRP3 Pathway

Mechanisms of Qingyi Decoction in Severe Acute Pancreatitis-Associated Acute Lung Injury via Gut Microbiota: Targeting the Short-Chain Fatty Acids-Mediated AMPK/NF-κB/NLRP3 Pathway

Ferroptosis in Rat Lung Tissue during Severe Acute Pancreatitis-Associated Acute Lung Injury: Protection of Qingyi Decoction

DIA-Based Proteomic Analysis of Plasma Protein Profiles in Patients with Severe Acute Pancreatitis

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