DIA-Based Proteomic Analysis of Plasma Protein Profiles in Patients with Severe Acute Pancreatitis

Abstract

Acute pancreatitis (AP) is a pancreatic inflammatory disease that varies greatly in course and severity. To further the understanding of the pathology of AP, we carried out data-independent acquisition-based proteomic analyses using proteins extracted from the plasma of patients with severe acute pancreatitis (SAP) (experimental group) and healthy volunteers (control group). Compared to the control group, there were 35 differentially expressed proteins (DEPs) in the plasma of patients with SAP. Of those, the expression levels for 6 proteins were significantly increased, and 29 proteins were significantly decreased. Moreover, six candidate biomarkers—VWF, ORM2, CD5L, CAT, IGLV3-10, and LTF—were matched as candidate biomarkers of the disease severity of AP. The area under the receiver operating characteristic of 0.903 (95% CI: 0.839, 0.967) indicated that this combination of these six candidate biomarkers had a good prediction accuracy for predicting the severity of AP. Our study provides specific DEPs that may be useful in the diagnosis and prognosis of SAP, which suggests new theoretical bases for the occurrence and development of SAP and offers potential novel treatment strategies for SAP.

Role of increased IGFBP2 in trophoblast cell proliferation and recurrent spontaneous abortion development: A pilot study

“…According to previous studies (Li et al., 2022 ; van Zundert et al., 2023 ), the quantified protein (60 μg) of each sample was added into a centrifuge tube, and then added dithiothreitol (5 μL of 1 M) the sample following incubation at 37°C for 1 h. Iodoacetamide (20 μL of 1 M) was added to the sample. After another incubation in the dark at room temperature for 1 h, the samples were centrifuged at 14,000 × g for 20 min at 4°C.…”

Hemorheological and Microcirculatory Relations of Acute Pancreatitis

“…The central events are the release of proinflammatory mediators (cytokines, vasoactive substances, free oxygen radicals) and the deterioration of microcirculation, and the activation of leukocytes and their rolling and infiltration into the tissue. These mediators are produced in the pancreas in the initial period of pancreatitis but later in the liver, lungs, and spleen as well [ 3 , 5 , 9 , 18 , 19 , 20 , 21 ].…”

Temporal Proteomic and Lipidomic Profiles of Cerulein-Induced Acute Pancreatitis Reveal Novel Insights for Metabolic Alterations in the Disease Pathogenesis

“…Systematic proteomic studies have considerably accelerated the hunt for biomarkers and revealed disease mechanisms in earlier pancreatic disease research. − Particularly, the advent of high-throughput proteomics based on LC–MS/MS has elucidated hundreds of divergent proteins and effectively addressed the workflow issue of pancreatic protein degradation . Numerous important up- and down-regulated proteins associated with inflammation, digestive enzymes, metabolism, signal transduction, and other processes were identified by these quantitative proteomic analyses in conjunction with functional annotations. − However, we were unable to fully comprehend the pathophysiology of AP due to the inconsistent protein changes obtained from CER − , or sodium taurocholate (NaT)-induced AP rats at a single time point.…”