The pathophysiological mechanisms of acute pancreatitis
(AP) are
complex and have remained a mystery to date, but metabolism is gradually
recognized as an important driver of AP onset and development. We
used a cerulein-induced AP mouse model to conduct liquid chromatography–mass
spectrometry (LC–MS/MS)-based time-course proteomics and lipidomics
in order to better understand the underlying metabolic alterations
linked with AP. Results showed that a series of significant changes
in proteins over time with a boost in expression were enriched in
lipase activity, lipoprotein, and lipids absorption and transport
regulation. Furthermore, 16 proteins associated with lipid metabolism
and signaling pathways together with the whole lipid species changing
profile led to the vital identification of changing law in glycerides,
phosphoglycerides, and free fatty acids. In addition to lipid metabolism
and regulation-associated proteins, several digestive enzymes and
adaptive anti-trypsin, stress response, and energy metabolism-related
proteins showed an increment in abundance. Notably, central carbon
and branched chain amino acid metabolism were enhanced during 0–24
h from the first cerulein stimulation. Taken together, this integrated
proteomics and lipidomics revealed a novel metabolic insight into
metabolites transforming rules that might be relevant to their function
and drug targets investigation. (Created with .)