Proteomic analysis of the skin of Chinese giant salamander (Andrias davidianus)

Geng, Xiaofang; Wei, Hong; Shang, Haitao; Zhou, Minghui; Chen, Bing; Zhang, Fuchun; Zang, Xiayan; Li, Pengfei; Sun, Jingyan; Che, Jing; Zhang, Yaping; Xu, Chi

doi:10.1016/j.jprot.2015.02.008

Cited by 45 publications

(32 citation statements)

References 53 publications

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“…In this study, myosins, dyneins, tyrosinase and glucocorticoid receptor were highly expressed in the newt skin. Myosins and dyneins participated in melanosome transport (Sugden et al, 2004;Geng et al, 2015b). Tyrosinase as the key regulatory enzyme of melanogenesis played a major role in skin color (Chen et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…However, the molecular basis of skin functions in urodele amphibians is still unclear. Unfortunately, our previous study of giant salamander skin (Geng et al, 2015b) was unable to comprehensively elucidate the molecular basis of skin functions and adaptation. Thus, this study attempted to employ a proteomic method to establish the skin proteomes of Chinese fire-bellied newt, and then compared the results to the skin proteomes of Chinese giant salamander Andrias davidianus obtained previously.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander

Sun

Geng

Guo

et al. 2016

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander

Sun

Geng

Guo

et al. 2016

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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“…Isoelectric focusing (IEF) was performed using Ettan IPGphor according to operating manual and the method previously described (Geng et al, 2015). Briefly, 1000 µg protein samples was applied to 24-cm pH 3-10 NL immobilized pH gradient gel strips in this study.…”

Section: -D Gel Electrophoresismentioning

confidence: 99%

“…The selected protein spots were manually excised, decolorized, dehydrated, and whitened according the method previously described (Geng et al, 2015). The dried protein spot was digested with 0.01 mg/mL trypsin at 37°C overnight.…”

Section: Protein Identification By Msmentioning

confidence: 99%

Proteome analysis of the liver in the Chinese fire-bellied newt Cynops orientalis

et al. 2016

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ABSTRACT. The Chinese fire-bellied newt, Cynops orientalis, belonging to Amphibia, Caudata, Salamandridae is a species endemic to China. The liver, which is an important digestive gland and the largest amphibian organ, has various functions, including detoxification, glycogen storage, protein synthesis, and hormone production. However, the newt liver has rarely been studied at the molecular level. We performed histomorphology and high-throughput proteomic analysis of the Chinese fire-bellied newt liver, using hematoxylin and eosin (H&E) staining and two-dimensional electrophoresis coupled with mass spectrometry. The H&E staining showed that the newt liver nuclei are large and round, are located in the lateral cytoplasm, and contain a large quantity of lipid droplets. Melanins were abundantly present throughout the hepatic parenchyma. The proteome analysis showed a total of 545 proteins detected in the newt liver. Furthermore, a gene ontology analysis suggested that these proteins were associated with metabolism, immune response, cellular homeostasis, etc. Among these, proteins with metabolic functions were found to be the most abundant and highly expressed. This supports the role of the liver as the metabolic center. The proteomic results provide new insights into the aspects of the liver proteomes of the Chinese fire-bellied newt. The identification of a more global liver proteome in the newt may provide a basis for characterizing and comparing the liver proteomes from other amphibian species.

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“…In order to characterize the expression patterns of the proteins identified in our quantitative iTRAQ data, Cluster 3.0/TreeView software was used for hierarchical clustering of the differentially expressed proteins as described in detail previously (Geng et al, 2015c).…”

Section: Bioinformatics Analysismentioning

confidence: 99%

iTRAQ-based proteomic analysis of adaptive response in the regenerating limb of the Cynops orientalis newt

Geng¹,

Guo²,

Zang³

et al. 2015

Int. J. Dev. Biol.

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The newt has the powerful capacity to regenerate lost limbs following amputation, and represents an excellent model organism to study regenerative processes. However, the molecular basis of the adaptive response in the regenerating limb of the Chinese fire-bellied newt Cynops orientalis immediately after amputation remains unclear. To better understand the adaptive response immediately after limb amputation at the protein level, we used isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS methods to analyze changes in the proteome of the regenerating newt limb that occurred 2 h and 8 h after amputation. We identified 152 proteins with more than 1.5-fold change in expression compared to control. GO annotation analysis classified these proteins into several categories such as signaling, Ca 2+ binding and translocation, transcription and translation, immune response, cell death, cytoskeleton, metabolism, etc. Further ingenuity pathway analysis (IPA) showed that several signaling pathways were significantly changed at 2 h and 8 h after amputation, including EIF2 signaling, acute phase response signaling, tight junction signaling and calcium signaling, suggesting these pathways may be closely related to the adaptive response immediately after limb amputation. This work provides novel insights into understanding the molecular processes related to newt limb regeneration immediately after amputation, and a basis for further study of regenerative medicine. KEY WORDS: Cynops orientalis, limb regeneration, proteomic, stress response, cell deathAmong vertebrates, urodele salamanders possess remarkable capability to regenerate appendages from any level of amputation through blastema formation. Subsequently, blastema cells that morphologically resemble mesenchymal stem-like cells self-organize into the amputated limb parts , Nye et al., 2003. Adult newt has already been used as an important model for the limb regeneration studies. The process of limb regeneration can be divided into three major phases: wound healing and dedifferentiation; blastema accumulation and blastema growth; differentiation and morphogenesis (Iten and Bryant, 1973). Following amputation, the wound surface is covered rapidly by epithelial cells, which form the wound epidermis at the end of the stump. More importantly, a specialized epithelium provides signals to the underlying cells of Int. J. Dev. Biol. 59: 487-496 (2015) Abbreviations used in this paper: iTRAQ , isobaric tags for relative and absolute quantitation; IPA, ingenuity pathway analysis.

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Proteomic analysis of the skin of Chinese giant salamander (Andrias davidianus)

Cited by 45 publications

References 53 publications

Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander

Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander

Proteome analysis of the liver in the Chinese fire-bellied newt Cynops orientalis

iTRAQ-based proteomic analysis of adaptive response in the regenerating limb of the Cynops orientalis newt

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