Proteome analysis of the liver in the Chinese fire-bellied newt Cynops orientalis

Zang, Xiayan; Guo, Jiuxin; Geng, Xiaofang; Li, P F; Sun, Jingyan; Wang, Q W; Xu, Chi

doi:10.4238/gmr.15037993

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…Liver, the major reticuloendothelial phagocytic system, recorded significant levels of Zn, especially after 4 weeks of injection in the G3 experimental group (89.38±1.53 µg/gm; Figure-3; table 5). This is attributed to the highly capturing and retaining capacity [50 -51] of Kupffer cells mainly, followed by hepatocytes and endothelial cells [52][53]. Important concentrations of Zn were observed in spleen after 2 and 4 weeks of injection in all groups (with a range of 5.94±0.18 µg/gm -79.38 ±0.51 µg/gm ).…”

Section: Bioaccumulation Studymentioning

confidence: 98%

Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

Razooki¹,

Rabee

2020

eijs

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The acute and sub chronic toxicity effects of 25.16 nm intraperitoneally- injected zinc oxide nanoparticles (ZnO NPs) were evaluated. Albino male mice were exposed to three different doses (25, 50 ,and 100 mg/kg ), depending on the value of calculated LD50, for 2 and 4 weeks . Considerable changes in organ indexes were shown with a good relevance to the illustrated histopathological effects which ranged from multiple hemorrhagic foci in liver, mild swelling and dilatation in kidney tubules, thickening of intestinal villi, moderate interstitial pneumonia, especially with the high dose , and sever necrosis of seminiferous tubules in testes of all studied groups. Significant changes in both hematological and biochemical parameters as well as thyroid hormones were observed with a considerable increase in the levels of antioxidant enzymes, in dose and exposure time dependent manner. The highest accumulated Zn mean values were recorded in the small intestine, kidney, liver, and spleen, respectively, followed by testes , heart , lung , and brain. These values followed the same order of the dose dependent manner, which explains the adverse effects that were recorded. This study proved the ability of using organ indexes as good tools side by side with the biochemical indicators to explain the histopathological changes. This study also revealed some histopathological effects that were not previously recorded as a toxicological effect of ZnO NPs in animal models.

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Section: Bioaccumulation Studymentioning

confidence: 98%

Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

Razooki¹,

Rabee

2020

eijs

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“…To further explore the mechanisms of amphibian response to low temperature, we selected Dryophytes and Hyla as the research targets for a study of mitochondrial gene expression. Liver is a main organ of energy metabolism in organisms [ 59 ], and studies have proven that using liver as a research model can obtain significant expression results [ 55 ]. Therefore, in this study, liver was selected as the main organ with which to explore the situation of mitochondrial gene expression under the stress of low temperature.…”

Section: Introductionmentioning

confidence: 99%

Differential Mitochondrial Genome Expression of Four Hylid Frog Species under Low-Temperature Stress and Its Relationship with Amphibian Temperature Adaptation

Hong,

Yuan,

et al. 2024

IJMS

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Extreme weather poses huge challenges for animals that must adapt to wide variations in environmental temperature and, in many cases, it can lead to the local extirpation of populations or even the extinction of an entire species. Previous studies have found that one element of amphibian adaptation to environmental stress involves changes in mitochondrial gene expression at low temperatures. However, to date, comparative studies of gene expression in organisms living at extreme temperatures have focused mainly on nuclear genes. This study sequenced the complete mitochondrial genomes of five Asian hylid frog species: Dryophytes japonicus, D. immaculata, Hyla annectans, H. chinensis and H. zhaopingensis. It compared the phylogenetic relationships within the Hylidae family and explored the association between mitochondrial gene expression and evolutionary adaptations to cold stress. The present results showed that in D. immaculata, transcript levels of 12 out of 13 mitochondria genes were significantly reduced under cold exposure (p < 0.05); hence, we put forward the conjecture that D. immaculata adapts by entering a hibernation state at low temperature. In H. annectans, the transcripts of 10 genes (ND1, ND2, ND3, ND4, ND4L, ND5, ND6, COX1, COX2 and ATP8) were significantly reduced in response to cold exposure, and five mitochondrial genes in H. chinensis (ND1, ND2, ND3, ND4L and ATP6) also showed significantly reduced expression and transcript levels under cold conditions. By contrast, transcript levels of ND2 and ATP6 in H. zhaopingensis were significantly increased at low temperatures, possibly related to the narrow distribution of this species primarily at low latitudes. Indeed, H. zhaopingensis has little ability to adapt to low temperature (4 °C), or maybe to enter into hibernation, and it shows metabolic disorder in the cold. The present study demonstrates that the regulatory trend of mitochondrial gene expression in amphibians is correlated with their ability to adapt to variable climates in extreme environments. These results can predict which species are more likely to undergo extirpation or extinction with climate change and, thereby, provide new ideas for the study of species extinction in highly variable winter climates.

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“…For frogs, proper brain functions and adaptations to low temperature play a crucial role in coordinating stress responses [ 8 ]. In addition, liver is an important digestive gland and also the largest organ of amphibians, with the status of a metabolic center [ 9 ]. Kidney is also a very important organ that uses a lot of energy for ion regulation and excretion of water and waste products [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial gene expression in different organs of Hoplobatrachus rugulosus from China and Thailand under low-temperature stress

Jin¹,

Guan²,

Dai³

et al. 2022

BMC Zool

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Background Hoplobatrachus rugulosus (Anura: Dicroglossidae) is distributed in China and Thailand and the former can survive substantially lower temperatures than the latter. The mitochondrial genomes of the two subspecies also differ: Chinese tiger frogs (CT frogs) display two identical ND5 genes whereas Thai tiger frogs (TT frogs) have two different ND5 genes. Metabolism of ectotherms is very sensitive to temperature change and different organs have different demands on energy metabolism at low temperatures. Therefore, we conducted studies to understand: (1) the differences in mitochondrial gene expression of tiger frogs from China (CT frogs) versus Thailand (TT frogs); (2) the differences in mitochondrial gene expression of tiger frogs (CT and TT frogs) under short term 24 h hypothermia exposure at 25 °C and 8 °C; (3) the differences in mitochondrial gene expression in three organs (brain, liver and kidney) of CT and TT frogs. Results Utilizing RT-qPCR and comparing control groups at 25 °C with low temperature groups at 8 °C, we came to the following results. (1) At the same temperature, mitochondrial gene expression was significantly different in two subspecies. The transcript levels of two identical ND5 of CT frogs were observed to decrease significantly at low temperatures (P < 0.05) whereas the two different copies of ND5 in TT frogs were not. (2) Under low temperature stress, most of the genes in the brain, liver and kidney were down-regulated (except for COI and ATP6 measured in brain and COI measured in liver of CT frogs). (3) For both CT and TT frogs, the changes in overall pattern of mitochondrial gene expression in different organs under low temperature and normal temperature was brain > liver > kidney. Conclusions We mainly drew the following conclusions: (1) The differences in the structure and expression of the ND5 gene between CT and TT frogs could result in the different tolerances to low temperature stress. (2) At low temperatures, the transcript levels of most of mitochondrial protein-encoding genes were down-regulated, which could have a significant effect in reducing metabolic rate and supporting long term survival at low temperatures. (3) The expression pattern of mitochondrial genes in different organs was related to mitochondrial activity and mtDNA replication in different organs.

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Proteome analysis of the liver in the Chinese fire-bellied newt Cynops orientalis

Cited by 6 publications

References 30 publications

Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

Differential Mitochondrial Genome Expression of Four Hylid Frog Species under Low-Temperature Stress and Its Relationship with Amphibian Temperature Adaptation

Mitochondrial gene expression in different organs of Hoplobatrachus rugulosus from China and Thailand under low-temperature stress

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