Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander

Sun, Jingyan; Geng, Xiaofang; Guo, Jiuxin; Zang, Xiayan; Li, Pengfei; Liu, Deming; Xu, Chi

doi:10.1016/j.cbd.2016.06.004

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…The Chinese giant salamander (CGS, Andrias davidianus ) is the largest extant amphibian in the world [ 24 , 25 ], belonging to the Cryptobranchidae family, which only contains three species: ( Cryptobranchus alleganiensisin in North America, Andrias japonicus in Japan, and Andrias davidianus in China). As an endangered species, the CGS has received increasing attention in evolutionary, comparative biology, and other studies [ 26 - 29 ]. Although there are morphological differences between urodele testis and mammal testis, the spermatogenetic process in urodele amphibians is similar to that of mammals.…”

Section: Introductionmentioning

confidence: 99%

UCHL1 expression and localization on testicular development and spermatogenesis of Chinese giant salamanders

Wang

Gao

et al. 2017

Oncotarget

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Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1), which is extensively expressed in vertebrates, is a deubiquitinating enzymes that inhibits the degradation of proteins by reversing ubiquitination modification. Herein, a 1087-bp sequence encoding UCHL1 was identified from the Chinese giant salamander (CGS; Andrias davidianus). The coding sequences (CDS) of UCHL1 encoded a putative poly peptide of 222 amino acids. The CGS UCHL1 isoforms were more related to their human and mouse counterparts. The phylogenic tree of vertebrate UCHL1 indicated that CGS UCHL1 has the closest relationship with human UCHL1 (up to 73.99 %). Before the gonads of male CGSs matured, the peak level of UCHL1 expression in testes appeared in 3-year-old CGSs according to RT-qPCR and western blot. In adult testes, the level of UCHL1 protein was lower in the breeding period than in the post-breeding period, whereas the level of UCHL1 protein in interstitial fluid of adult CGS testes was higher during the breeding period than during the post-breeding period. In testicular seminiferous lobules in the developmental stage of CGSs, immunohistochemistry displayed three kinds of localizing patterns of UCHL1, including nuclear localization at half year old, cytoplasmic localization from one year to three years old, and extracellular localization in adult. In testicular seminiferous lobules of adult CGS, the different developmental germ cells were separated by cysts containing UCHL1 protein, but UCHL1 did not localize on the mature sperm. The results showed that extracellular UCHL1 loaded on exosomes, as a component of the homogeneous germ cell cysts, could regulate the synchronous development of sperm in testes of adult CGS.

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Section: Introductionmentioning

confidence: 99%

UCHL1 expression and localization on testicular development and spermatogenesis of Chinese giant salamanders

Wang

Gao

et al. 2017

Oncotarget

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“…When the skin of giant salamander is stimulated to a certain degree, it will secrete some white mucus, the main components of which are protein, amino acid, mucopolysaccharide and antimicrobial peptide [ 4 ]. According to the folk experience, these mucus powders have a remarkable effect on wounds and burns [ 3 ]. The group used scratching and stimulation to collect the salamander skin mucus, vacuum freeze-dried it, and crushed the salamander skin secretion with a low-temperature ultra micro crusher to get tiny granular salamander secretion after crushing ( Figure 2A ).…”

Section: Resultsmentioning

confidence: 99%

“…When the salamander encounters external stimuli such as low-voltage electric current or needles, the surface of its body will secrete a kind of mucus [3]. When technicians apply a powder made from the mucus to animal wounds, they find that the wounds heal quickly and automatically, stopping bleeding instantly, and find that there is only a thin line on the surface of the bonded wounds, which are very firmly bonded [3][4][5][6]. It was reported that salamander mucus can be used as a surgical adhesive [7].…”

Section: Introductionmentioning

confidence: 99%

Improvement of skin wound healing by giant salamander skin mucus gel wrapped with bone marrow mesenchymal stem cells via affecting integrin family molecules

Li,

Du,

Huang

et al. 2024

Aging

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Background: Traditional bandages, gauze, and cotton balls are increasingly insufficient for addressing complex war injuries characterized by severe bleeding and diverse wound conditions. The giant salamander, a species of high medical value, secretes a unique mucus when stimulated, which has potential applications in wound care. Materials: Giant salamander skin mucus gel dressing wrapped with bone marrow mesenchymal stem cells (BMSCs-GSSM-gel) was prepared and validated. Skin wound injury of rabbit and mouse models were established. Hematoxylin and Eosin, Masson's trichrome, and Sirius red staining were performed. The platelet aggregation rate and coagulation items were measured. Transcriptome sequencing was performed to find potential differential expression genes. Results: Preparation and characterization of BMSCs-GSSM-gel were performed, and BMSCs-GSSM-gel particles with a diameter of about 200 nm were obtained. BMSCs-GSSM-gel accelerated wound healing in both rabbit and mouse models. BMSCs-GSSM-gel significantly promoted hemostasis via increasing platelet aggregation rate and fibrinogen, but decreasing activated partial thromboplastin time, thrombin time, and prothrombin time. BMSCs-GSSM-gel treatment significantly impacted several genes associated with cell adhesion, inflammatory response, collagen-containing extracellular matrix, and the positive regulation of cell migration based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Integrin Subunit Beta 4 (ITGB4), Integrin Subunit Alpha 3 (ITGA3), and Laminin Subunit Beta 3 (LAMB3) might be involved in the wound healing process by BMSCs-GSSM-gel. Conclusions: We proved the BMSCs-GSSM-gel greatly improved the skin wound healing, and it might play a crucial role in the application fields of skin damage repair.

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“…Recently, natural bioactive substances, including peptides, polyphenols, and polysaccharides, have been revealed to be effective in the prevention and treatment of HUA and CKD [6,12] . The Chinese giant salamander (Andrias davidianus) is an economically important animal with Chinese characteristics, leading to the establishment of artificial breeding and legal large-scale production in China for protection and rational utilization of the cultivated species [13] . It has been demonstrated that bioactive peptides, collagen, chondroitin sulfate, functional oils, and other compounds derived from A. davidianus display various bioactivities, including anti-oxidant, anti-tumor, anti-ageing and anti-fatigue activities, among others [14] .…”

Section: Food Science and Human Wellnessmentioning

confidence: 99%

Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo

Li,

Chen,

Chen

et al. 2024

Food Science and Human Wellness

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Hyperuricemia (HUA) is a vital risk factor for chronic kidney diseases (CKD) and development of functional foods capable of protecting CKD is of importance. This paper aimed to explore the amelioration effects and mechanism of Andrias davidianus bone peptides (ADBP) on HUA-induced kidney damage. In the present study, we generated the standard ADBP which contained high hydrophobic amino acid and low molecular peptide contents. In vitro results found that ADBP protected uric acid (UA)-induced HK-2 cells from damage by modulating urate transporters and antioxidant defense. In vivo results indicated that ADBP effectively ameliorated renal injury in HUA-induced CKD mice, evidenced by a remarkable decrease in serum UA, creatinine and blood urea nitrogen, improving kidney UA excretion, antioxidant defense and histological kidney deterioration. Metabolomic analysis highlighted 14 metabolites that could be selected as potential biomarkers and attributed to the amelioration effects of ADBP on CKD mice kidney dysfunction. Intriguingly, ADBP restored the gut microbiome homeostasis in CKD mice, especially with respect to the elevated helpful microbial abundance, and the decreased harmful bacterial abundance. This study demonstrated that ADBP displayed great nephroprotective effects, and has great promise as a food or functional food ingredient for the prevention and treatment of HUA-induced CKD.

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Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander

Cited by 9 publications

References 27 publications

UCHL1 expression and localization on testicular development and spermatogenesis of Chinese giant salamanders

UCHL1 expression and localization on testicular development and spermatogenesis of Chinese giant salamanders

Improvement of skin wound healing by giant salamander skin mucus gel wrapped with bone marrow mesenchymal stem cells via affecting integrin family molecules

Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo

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