Proteomic analysis of Rhizoctonia solani AG-1 sclerotia maturation

Kwon, Young Chul; Kim, Pil Joo; Chung, Woo Sik; Bae, Hyeun‐Jong; Jeong, Sung Woo; Shin, Sung Chul; Jeong, Mi‐Jeong; Park, Soo-Chul; Kwak, Youn‐Sig; Bae, Dong-Won; Lee, Yong-Bok

doi:10.1016/j.funbio.2014.02.001

Cited by 32 publications

(32 citation statements)

References 23 publications

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“…Other factors that may determine fungal success include their resilience in the presence of natural or anthropogenic stressors (e.g., heavy metals, xenoorganics; Krauss et al 2011; links between freezing tolerance and trade-offs in Arabidopsis thaliana, Oakley et al 2014); response to leaf-eating invertebrates or competing bacteria or fungi (in the seaweed Fucus vesiculosus, over 900 genes were up or down regulated in the presence of herbivores; Fl€ othe et al 2014); changing gene expression when switching from growth to reproduction (as shown during the sclerotia maturation of Rhizoctonia solani; Kwon et al 2014). Temperature preferences, response to predators and competitors, and the switch from growth to reproduction are more complex traits than the ability to use certain substrates and are generally controlled by multiple genes, which makes extrapolations from the presence or expression of associated genes to their effect on the taxon's occurrence much more complex.…”

Section: Nutritional and Other Traitsmentioning

confidence: 99%

Aquatic hyphomycetes in a changing environment

Bärlocher

2016

Fungal Ecology

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Section: Nutritional and Other Traitsmentioning

confidence: 99%

Aquatic hyphomycetes in a changing environment

Bärlocher

2016

Fungal Ecology

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“…Therefore, the cell metabolism was reduced. In addition, six antioxidant enzymes (three SODs, dihydropteroate synthase, cytochrome C peroxidase, and alpha-ketoglutaratedependent taurinedioxygenase) of R. solani were differently expressed during sclerotial maturation (Kwon et al 2014). In this study, SOD, dihydropteroate synthase, and cytochrome C peroxidase were found to be differentially expressed among all three stages, and more antioxidant genes were found to accumulate during the sclerotia formation process, including CAT, putative protein disulfide-isomerase, and glutathione peroxidase.…”

Section: Discussionmentioning

confidence: 49%

“…As far as we know, this is the first transcriptome analysis on maize sheath blight (R. solani AG1 IA) during the sclerotium formation process. Kwon et al (2014) analyzed proteomic changes during the process of sclerotial formation of R. solani for 5 days, 7 days, and days, respectively, and obtained 55 differentially expressed proteins, representing high expression of processing, cellular processes, amino acid metabolism, cell defense, and carbohydrate metabolism during the sclerotium formation. In line with these previous findings, through RNAseq analysis during the formation of the sclerotium, we identified 5000-6000 DEGs between the three stages from the mycelial stage to the sclerotial initiation and maturation stages, and the majority of the highly expression were involved in the oxidation-reduction process, carbohydrate metabolic process, catalytic activity, and oxidoreductase activity.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #2 of "Transcriptomic evidence for involvement of reactive oxygen species in Rhizoctonia solani AG1 IA sclerotia maturation (v0.1)"

2018

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Rhizoctonia solani AG1 IA is a soil-borne fungal phytopathogen that can significantly harm crops resulting in economic loss. This species overwinters in grass roots and diseased plants, and produces sclerotia that infect future crops. R. solani AG1 IA does not produce spores; therefore, understanding the molecular mechanism of sclerotia formation is important for crop disease control. Toward this end, to identify the genes involved in this process for the development of disease control targets, in this study, the transcriptomes of this species were determined at three important developmental stages (mycelium, sclerotial initiation, and sclerotial maturation) using an RNA-sequencing approach. A total of 5016, 6433, and 5004 differentially expressed genes (DEGs) were identified in the sclerotial initiation vs. mycelial, sclerotial maturation vs. mycelial, and sclerotial maturation vs. sclerotial initiation stages, respectively. Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that these DEGs were enriched in diverse categories, including oxidoreductase activity, carbohydrate metabolic process, and oxidation-reduction processes . Twelve DEGs were further verified using reverse transcription quantitative PCR (RT-qPCR). Among the genes examined, NADPH oxidase 1 (NOX1) and superoxide dismutase (SOD) were highly induced in the stages of sclerotial initiation and maturation. In addition, the highest reactive oxygen species (ROS) production levels were detected during sclerotial initiation, and enzyme activities of NOX1, SOD, and catalase (CAT) matched with the gene expression profiles. To further evaluate the role of ROS in sclerotial formation , R. solani AG1 IA was treated with the CAT inhibitor aminotriazole and H 2 O 2 , resulting in the early differentiation of sclerotia . Taken together, this study provides useful information toward understanding the molecular basis of R. solani AG1 IA sclerotial formation and maturation, and identified the important role of ROS in these processes.

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“…solani mycelium maturation, which have proven to be efficient techniques. Proteomics studies revealed that during the maturation of sclerotia, 55 different types of proteins are differentially expressed and involved in various cellular functional metabolic pathways [19]. Genes and proteins associated with modifying host cell walls or host infection were revealed by transcriptomics and proteomics [20, 21].…”

Section: Introductionmentioning

confidence: 99%

Metabolites contributing to Rhizoctonia solani AG-1-IA maturation and sclerotial differentiation revealed by UPLC-QTOF-MS metabolomics

Pan

Abbas

et al. 2017

PLoS ONE

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Rhizoctonia solani is a causative agent of sheath blight, which results in huge economic losses every year. During its life cycle, the formation of sclerotia helps Rhizoctonia solani withstand a variety of unfavorable factors. Oxidative stress is a key factor that induces sclerotium formation. The differentiated and undifferentiated phenotypes of R. solani AG-1-IA were obtained by controlling aerial conditions. Metabolomics based on the mass spectrometry technique combined with multivariate and univariate analyses was used to investigate the metabolic variation in vegetative, differentiated and undifferentiated mycelia. Our results revealed that during maturation, the metabolic levels of N2-acetyl-L-ornithine, 3,1'-(OH)2-Gamma-carotene, (5Z,7E)-(1S,3R)-24,24-difluoro-24a-homo-9,10-seco-5,7,10(19)-cholestatrien-1,3,25-triol, stoloniferone O, PA(O-18:0/12:0), PA(P-16:0/14:0), PA(P-16:0/16:(19Z)) and PA(P-16:0/17:2(9Z,12Z)) were suppressed in both differentiated and undifferentiated mycelia. The concentrations of PE(20:1(11Z)/14:1(9Z)), PE(P-16:0/20:4(5Z,8Z,11Z,13E)(15OH[S])) and PS(12:0/18:1(9Z)) were increased in the differentiated group, while increased levels of N(gamma)-nitro-L-arginine, tenuazonic acid and 9S,10S,11R-trihydroxy-12Z,15Z-octadecadienoic acid were found in the undifferentiated group. Our results suggest that different levels of these metabolites may act as biomarkers for the developmental stages of R. solani AG-1-IA. Moreover, the mechanisms of sclerotium formation and mycelium differentiation were elucidated at the metabolic level.

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Proteomic analysis of Rhizoctonia solani AG-1 sclerotia maturation

Cited by 32 publications

References 23 publications

Aquatic hyphomycetes in a changing environment

Aquatic hyphomycetes in a changing environment

Peer Review #2 of "Transcriptomic evidence for involvement of reactive oxygen species in Rhizoctonia solani AG1 IA sclerotia maturation (v0.1)"

Metabolites contributing to Rhizoctonia solani AG-1-IA maturation and sclerotial differentiation revealed by UPLC-QTOF-MS metabolomics

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