Proteomic analysis of chromium cytotoxicity in cultured rat lung epithelial cells

Lei, Ting; He, Qing-Yu; Cai, Zhen; Zhou, Yuan; Wang, Yi Li; Sheng, Lu; Cai, Zongwei; Chiu, Jen-Fu

doi:10.1002/pmic.200701050

Cited by 38 publications

(20 citation statements)

References 35 publications

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“…shown to be up-regulated during ROS-related apoptosis (Lei et al, 2008). Both proteins were upregulated in the liver of Cd-treated M.musculus mice, corroborating the strong oxidative stress detected ( Fig.…”

Section: Stress Responsesupporting

confidence: 68%

Functional genomics and metabolomics reveal the toxicological effects of cadmium in Mus musculus mice

et al. 2015

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Abstract:Cadmium (Cd) is an environmental pollutant that accumulates in the organisms causing serious health problems. Over the past decades, "omics" studies have been conducted trying to elucidate changes in the genome, the transcriptome or the proteome after Cd exposure. Metabolomics is relatively new to the "omics" revolution, but has shown enormous potential for investigating biological systems or their perturbations. When metabolomic data are interpreted in combination with genomic, transcriptomic and proteomic results, in the co-called systems biology approach, a holistic knowledge of the organism/process under investigation can be achieved. In this work, transcriptional and proteomic analysis (functional genomics) were combined with metabolomic workflow to evaluate the biological responses caused in Mus musculus mice by Cd (subcutaneous injection for 10 consecutive days). Animals showed high Cd levels in liver and plasma, drastic lipid peroxidation in liver, increased transcription of hepatic genes involved in oxidative stress, metal transport, immune response and lipid metabolism and moderate decreases of DNA repair genes mRNAs. 2DE-DIGE proteomics confirmed changes of hepatic proteins related to stress and immune responses, or involved in energy metabolism, suggesting a metabolic switch in the liver from oxidative phosphorylation to aerobic glycolysis, that was confirmed by metabolomics analysis, via DIMS and GC-MS. This metabolic alteration is particularly important for highly proliferating cells, like tumor cells, which require a continuous supply of precursors for the synthesis of lipids, proteins and nucleic acids. The metabolic changes observed in mouse liver by metabolomics and the oxidative stress Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporationdetected via functional genomics could be in the base of Cd hepatocarcinogenicity. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation FUNCTIONAL GENOMICS AND METABOLOMICS REVEAL THE TOXICOLOGICAL EFFECTS OF CADMIUM IN Mus musculus MICE

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Section: Stress Responsesupporting

confidence: 68%

Functional genomics and metabolomics reveal the toxicological effects of cadmium in Mus musculus mice

et al. 2015

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“…Protein spots were visualized by silver staining as described previously [Lei et al, 2008]. The stained gels were scanned with LabScan 6.0 (GE Healthcare) and data were analyzed with Progenesis SameSpots (Nonlinear Dynamics, UK).…”

Section: Silver Staining and Image Analysismentioning

confidence: 99%

ERp57 is up‐regulated in free fatty acids‐induced steatotic L‐02 cells and human nonalcoholic fatty livers

Wang

Chan

Pan

et al. 2010

J of Cellular Biochemistry

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Pathogenesis of nonalcoholic fatty liver disease (NAFLD) is not clear. In this study we aimed to identify proteins involved in NAFLD development in free fatty acids (FFA)-induced hepatosteatotic cells and in human liver biopsies. Steatosis was induced by incubating a normal human hepatocyte-derived cell line L-02 with FFA. Differentially expressed proteins in the steatotic cells were analyzed by two-dimensional gel electrophoresis-based proteomics. Involvement of one of the up-regulated proteins in steatosis was characterized using the RNA interference approach with the steatotic cells. Protein expression levels in liver biopsies of patients with NAFLD were assessed by immunohistochemistry. Proteomic analysis of L-02 steatotic cells revealed the up-regulation of ERp57, a condition not previously implicated in NAFLD. Knockdown of ERp57 expression with siRNA significantly reduced fat accumulation in the steatotic cells. ERp57 expression was detected in 16 out of 17 patient biopsies and correlated with inflammation grades or fibrosis stages, while in 5 normal biopsies ERp57 expression was not detectable in hepatocytes. In conclusion, ERp57 was up-regulated in FFA-induced steatotic hepatic cells and in NAFLD patient livers and demonstrated steatotic properties in cultured cells. Further investigations are warranted to verify the involvement of ERp57 in NAFLD development.

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“…This in turn is responsible for defective hematopoiesis [12]. It was established that Cr (VI) is a strong oxidant which causes cellular dysfunction and cell death [4,6,[13][14][15]. The routes of excretion of chromium are via the kidney/urine and bile/faeces [17].…”

Section: Introductionmentioning

confidence: 99%

Protective effects of selenium against potassium dichromate-induced hematotoxicity in female and male Wistar albino rats

Adjroud

2010

Ann Toxicol Anal

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-Objective: Potassium dichromate (K 2 Cr 2 O 7 ) is a potent pollutant for human and animal health. The purpose of the current work is to compare the effect of K 2 Cr 2 O 7 using variations in the dose, route of administration and duration of exposure in male and female Wistar albino rats and to research the interaction of chromium and selenium with a special focus on hematopoiesis. Materials and methods: K 2 Cr 2 O 7 was subcutaneously administered alone (10, 50 and 100 mg/kg body weight) or K 2 Cr 2 O 7 (10 mg/kg) in association with selenium (0.3 mg/kg) was administered to female Wistar albino rats. Male rats received in their drinking water K 2 Cr 2 O 7 (30 mg/L/day) alone or in association with Se (0.3 mg/L/day) for 20 consecutive days. The hematological parameters were evaluated on days 3, 6 and 21 after subcutaneous (sc.) treatment in female rats and on days 10 and 20 after oral administration in male rats. Results: K 2 Cr 2 O 7− induced during the first three days a significant (p < 0.05) dose-dependent decrease in the number of erythrocytes, platelets, leucocytes, lymphocytes and the hematocrit levels, and a dose-dependent increase in the number of granulocytes and monocytes. In the drinking water, chromium sc. significantly decreased the number of leucocytes and lymphocytes on day 10 after treatment and elevated the number of granulocytes and monocytes 20 days later. Selenium sc. counterbalanced the hematotoxic effects of chromium in female rats. Conclusion: These results suggest that the selenium has a protective role against the hematotoxicity of subcutaneous chromium in female Wistar rats.Key words: Chromium, rat, selenium, hematotoxicity Résumé -Objectif : Le dichromate de potassium (K 2 Cr 2 O 7 ) est un polluant potentiellement néfaste pour la santé humaine et animale. Cette étude a été entreprise afin de rechercher une éventuelle interaction entre le K 2 Cr 2 O 7 et le sélénium (Se) sur l'hématopoïèse chez les rats albinos Wistar mâles et femelles. Matériel et Méthodes : Le K 2 Cr 2 O 7 est administré seul (10, 50 et 100 mg/kg) par voie sous-cutanée (sc) ou en association (10 mg/kg) avec le sélénium (0,3 mg/kg). Les rats mâles reçoivent dans l'eau de boisson le K 2 Cr 2 O 7 (30 mg/L/jour) seul ou en association avec le sélénium (0,3 mg/L/jour) pendant 20 jours consécutifs. Les variations des paramètres hématologiques sont évaluées au 3 e , 6 e et 21 e jour chez les rattes et au 10 e et 20 e jour chez les mâles. Résultats : Les résultats montrent que le chrome engendre dès le 3 e jour après son administration par voie sc chez la femelle une diminution notable et dose-dépendante (p < 0,05) du nombre d'érythrocytes, du taux d'hématocrite, du nombre de plaquettes sanguines, de leucocytes, de lymphocytes et une augmentation dose-dépendante du nombre de granulocytes et de monocytes. Le chrome administré par voie orale diminue le nombre de leucocytes et de lymphocytes dès le 10 e jour du traitement et augmente celui des monocytes et des granulocytes 20 jours plus tard. La présence du sélénium ...

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Proteomic analysis of chromium cytotoxicity in cultured rat lung epithelial cells

Cited by 38 publications

References 35 publications

Functional genomics and metabolomics reveal the toxicological effects of cadmium in Mus musculus mice

Functional genomics and metabolomics reveal the toxicological effects of cadmium in Mus musculus mice

ERp57 is up‐regulated in free fatty acids‐induced steatotic L‐02 cells and human nonalcoholic fatty livers

Protective effects of selenium against potassium dichromate-induced hematotoxicity in female and male Wistar albino rats

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