Proteome profiling of L3 and L4 Anisakis simplex development stages by TMT-based quantitative proteomics

Proteomic Profiling Reveals New Insights into the Allergomes of Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum

“…Here, we performed LC-MS/MS profiling of L3 larvae of A. simplex, P. decipiens, and Contracaecum osculatum. To date, few studies have explored the proteomes of A. simplex and P. decipiens (Arcos et al, 2014;Faeste et al, 2014;Carrera et al, 2016;Stryin´ski et al, 2019), whereas the proteome of C. osculatum was examined for the first time in the present survey. We predicted a higher number of A. simplex proteins (n ¼ 645) compared with P. decipiens (n ¼ 397) and C. osculatum (n ¼ 261) proteins probably because the protein database for Anisakis is much larger because, in contrast to Pseudoterranova and Contracaecum, the whole genome of Anisakis was sequenced.…”

“…Nonetheless, Carrera et al (2016) identified 1,231 and 1,276 proteins in A. simplex and P. decipiens L3, respectively, using a gel-free approach. Moreover, Stryin´ski et al (2019) reported 1,872 proteins of A. simplex L3. Carrera et al (2016) and Stryin´ski et al (2019) probably predicted a larger number of proteins because they used a LC-MS/MS system with better resolution and different search engines.…”

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

“…Most of all, A. simplex allergens have been identified and characterized [ 248 ]. Recently, for the first time, the global proteome of the third and fourth stage larvae of A. simplex was analyzed using quantitative proteomics based on tandem mass tag (TMT) [ 31 ]. In addition, the response to the invasive larvae of A. simplex s.s. to ivermectin (anthelminthic drug) was also evaluated using TMT-based methodology [ 250 ].…”

“…In addition, identifying potential vaccination targets (proteins) appears to be one of the leading ways to control parasitic diseases [ 30 ]. Accurate knowledge and description of the mechanism of action of these proteins can be used in the research on antiparasitic drugs, and help in combating FBPs through detection and/or neutralization [ 31 ]. At the same time, scientists are making great efforts to clarify the sensitization mechanism of various allergenic proteins from food sources, where allergic reactions to food are more often caused by FBP allergens contaminating food products [ 3 , 32 , 33 ].…”

Characterization of the Jumbo Squid (Dosidicus gigas) Skin By-Product by Shotgun Proteomics and Protein-Based Bioinformatics

“…Proteomics, as the discipline for the large-scale analysis of proteins of a particular biological system, has greatly contributed to the assessment of quality, safety, and bioactivity of seafood products [21][22][23][24]. In a shotgun proteomics approach, a mixture of proteins is digested with a protease (i.e., trypsin), and the resulting mixture of peptides is then analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) [25].…”