Proteomic Profiling Reveals New Insights into the Allergomes of Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum

Kochanowski, Maciej; Dąbrowska, Joanna; Różycki, Mirosław; Karamon, Jacek; Sroka, Jacek; Cencek, Tomasz

doi:10.1645/19-75

Cited by 24 publications

(19 citation statements)

References 44 publications

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“…Additionally, the similar reaction to ES proteins observed for both parasites would suggest a high similarity between Anisakis and Contracaecum IgE-epitopes, since specific anisakis -IgE generally reacts more specifically to these kind of proteins [ 5 , 27 , 28 , 29 ]. This fact would be also confirmed by the numerous Contracaecum protein bands that were revealed in the WB analysis, recognized by specific Anisakis IgE from sera in this work, and is in agreement with previous works by IgG-ELISA-based serodiagnosis [ 18 ] and by proteomic profiling [ 19 ] with both species. However, personal responses have not been studied in this work by in vitro methods, and it should be stated that the individual response against A. simplex and C. osculatum antigens could differ depending on the individual patients with allergic anisakidosis, as observed by in vivo methods.…”

Section: Discussionsupporting

confidence: 93%

“…In the last 20 years, it has become apparent that A. simplex is one of the most important hidden food allergens in the adult population suffering acute urticaria and anaphylaxis [ 30 ]. Regarding the results presented in this and previous works [ 19 ], and the practical lack of SPT-response from the other studied parasites from freshwater ( Tetracotyle sp., Apophallus sp. and Posthodiplostomum sp.…”

Section: Discussionmentioning

confidence: 58%

“…As said previously, Anisakis simplex antigens are known to potentially cross-react with other allergens from, e.g., other ascarid nematodes [ 3 , 4 ] and with molecules from mites, crustaceans and mollusks [ 5 , 6 , 7 , 8 ]. However, although information on potential similar allergens in other anisakid nematodes has been hypothesized [ 18 , 19 ], and IgG antigenic homology among nematodes has been described previously [ 3 , 18 ], in vivo and IgE cross-reactions between fish parasites within this family had not yet been studied. To our knowledge, this is the first time that IgE cross-reactivity between two related species, Anisakis simplex and Contracaecum osculatum , both present in fish, has been confirmed in some patients.…”

Section: Discussionmentioning

confidence: 99%

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Evidence of IgE-Mediated Cross-Reactions between Anisakis simplex and Contracaecum osculatum Proteins

et al. 2021

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Fish consumers may develop allergic reactions following the ingestion of fish products containing nematode larvae within the genus Anisakis. Sensitized patients may cross-react with proteins from insects, mites and mollusks, leading to allergic reactions even in the absence of the offending food. Potential cross-reactivity in Anisakis-allergic patients with larval proteins from other zoonotic parasites present in freshwater and sea fish should be investigated due to an increasing occurrence in certain fish stocks, particularly Contracaecum osculatum. In this work, we evaluated IgE-cross reactions by in vivo (skin prick tests with parasites extracts) and in vitro methods (IgE-ELISA and IgE-immunoblot). In vivo skin prick tests (SPT) proved the reactivity of Anisakis-sensitized patients when exposed to C. osculatum antigens. Sera from Anisakis-sensitized patients confirmed the reaction with somatic antigens (SA) and excretory/secretory proteins (ES) from C. osculatum. Only anecdotal responses were obtained from other freshwater worm parasites. Consequently, it is suggested that Anisakis-sensitized humans, especially patients with high levels of specific anti-Anisakis antibodies, may react to C. osculatum proteins, possibly due to IgE-mediated cross-reactivity.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

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Evidence of IgE-Mediated Cross-Reactions between Anisakis simplex and Contracaecum osculatum Proteins

et al. 2021

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“…In 2017, Ludovisi et al showed that intraperitoneal infection of mice with live P. decipiens L3 could induce a mixed T helper type (Th) 1/Th2 response and make mice react (i.e., irritability and reduced activity) to a subsequent oral challenge with P. decipiens proteins 7 . An allergenic potential of P. decipiens was also supported by a proteomic study by Kochanowski, et al, who revealed the presence of at least eight A. simplex -like allergens in crushed P. decipiens extract and predicted another 28 putative allergens in both A. simplex and P. decipiens by use of high-resolution liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) 8 . Such LC–MS/MS method had also been previously applied by Carrera, et al who identified one shared allergen (Ani s 9) between both nematode species 9 .…”

Section: Introductionmentioning

confidence: 75%

Targeted proteomics and specific immunoassays reveal the presence of shared allergens between the zoonotic nematodes Anisakis simplex and Pseudoterranova decipiens

Saelens

Planckaert

Martínez-Sernández

et al. 2022

Sci Rep

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The family Anisakidae, mainly represented by Anisakis simplex s.l. and Pseudoterranova decipiens, encompasses zoonotic nematodes infecting many marine fish. Both are responsible for gastrointestinal disease in humans after ingestion of a live larva by consumption of undercooked fish, and, in the case of A. simplex, an allergic reaction may occur after consuming or even handling infected fish. Due to its phylogenetic relatedness with A. simplex, few studies investigated the allergenic potential of P. decipiens, yet none of them focused on its excretory/secretory (E/S) proteins that easily get missed when working solely on extracts from crushed nematodes. Moreover, these E/S allergens remain behind even when the larva has been removed during fish quality processing. Therefore, the aim was to investigate if Anisakis-like allergens could also be detected in both crushed and E/S P. decipiens protein extract using targeted mass spectrometry analysis and immunological methods. The results confirmed that at least five A. simplex allergens have homologous proteins in P. decipiens; a result that emphasizes the importance of also including E/S protein extracts in proteomic studies. Not only A. simplex, but also P. decipiens should therefore be considered a potential source of allergens that could lead to hypersensitivity reactions in humans.

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“…The symptoms include nausea, vomiting, diarrhea, or an allergic reaction. These reactions are caused by chemical compounds found in the fish meat, which are produced by the parasite (Ivanovic et al 2017;Kochanowski et al 2020). Cases of anisakiasis that has been reported were mostly caused by A. simplex and A. pegrefii (Baird et al 2014;Aibinu et al 2019) meanwhile, those caused by A. typica are not yet widely known and receive relatively less attention, therefore, the zoonotic effect may also be underestimated (Umehara et al 2010).…”

Section: Discussionmentioning

confidence: 99%

The occurrence of Anisakis larvae on hairtail, Trichiurus lepturus caught from the Pangandaran Waters, West Java, Indonesia

et al. 2021

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Abstract. Ayun NQ, Dewi LS, Murwantoko, Setyobudi E. 2021. The occurrence of Anisakis larvae on hairtail, Trichiurus lepturus caught from the Pangandaran Waters, West Java, Indonesia. Biodiversitas 22: 1378-1384. This study aimed to investigate the prevalence, mean intensity, and type of Anisakis larvae infecting hairtail (Trichiurus lepturus) caught at Pangandaran Waters, West Java, Indonesia. It was conducted using 869 samples sourced from local fishermen. The parameters measured were the total length and weight of each T. lepturus. Furthermore, parasitological examination was carried out to investigate the occurrence of Anisakis larvae in the fishes. The selected samples of Anisakis were identified through morphological and molecular analysis. The results showed that the larvae were found either free in the abdominal cavity of the fishes or coiled and attached to the serosal layer of the gastrointestinal tract, gonad, liver, and muscle. The results showed that the larvae were found either free in the abdominal cavity of the fishes or coiled and attached to the serosal layer of the gastrointestinal tract, gonad, liver, and muscle. It also showed that their prevalence in the T. lepturus was at a moderate level (45.5%), with the mean intensity at 4.4 larvae/fish. Moreover, the larvae were mostly in the abdominal cavity (89%), as only a few were found in another part of the fishes. Morphological identification showed that the type of Anisakis present was type I, as they were characterized by the presence of mucron and ventriculus without caecum. Molecular analysis was carried out by sequencing the ITS region, and it confirmed that the nematode was Anisakis typica, a non-zoonotic parasite. These parasites were not present in the edible portion/muscle of the fishes, and had a relatively moderate prevalence and low intensity. Therefore, their presence posed a relatively low risk to human health. However, due to consumption patterns and the increasing popularity of seafood, being served raw or undercooked, the incidence of anisakiasis may increase in the future. In the other hand, the presence of these larvae might be used as a biological indicator for several ecological studies.

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Proteomic Profiling Reveals New Insights into the Allergomes of Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 24 publications

References 44 publications

Evidence of IgE-Mediated Cross-Reactions between Anisakis simplex and Contracaecum osculatum Proteins

Evidence of IgE-Mediated Cross-Reactions between Anisakis simplex and Contracaecum osculatum Proteins

Targeted proteomics and specific immunoassays reveal the presence of shared allergens between the zoonotic nematodes Anisakis simplex and Pseudoterranova decipiens

The occurrence of Anisakis larvae on hairtail, Trichiurus lepturus caught from the Pangandaran Waters, West Java, Indonesia

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