Proteome analysis of sugar beet leaves under drought stress

Abstract

Drought is one of the major factors limiting the yield of sugar beet (Beta vulgaris L.). The identification of candidate genes for marker-assisted selection (MAS) could greatly improve the efficiency of breeding for increased drought tolerance. Drought-induced changes in the proteome could highlight important genes. Two genotypes of sugar beet (7112 and 7219-P.69) differing in genetic background were cultivated in the field. A line-source sprinkler irrigation system was used to apply irrigated and water deficit treatments beginning at the four-leaf stage. At 157 days after sowing, leaf samples were collected from well-watered and drought-stressed plants for protein extraction and to measure shoot biomass and leaf relative water content. Changes induced in leaf proteins were studied by two-dimensional gel electrophoresis and quantitatively analyzed using image analysis software. Out of more than 500 protein spots reproducibly detected and analyzed, 79 spots showed significant changes under drought. Some proteins showed genotype-specific patterns of up- or downregulation in response to drought. Twenty protein spots were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), leading to identification of Rubisco and 11 other proteins involved in redox regulation, oxidative stress, signal transduction, and chaperone activities. Some of these proteins could contribute a physiological advantage under drought, making them potential targets for MAS.

Proteomics Analysis of Drought Stress-Responsive Proteins in Hippophae rhamnoides L.

“…It is well-known that drought can cause the decrease on synthesis of rubisco (Vu et al 1999) and a loss of rubisco activity (Parry et al 2002;Chaitanya et al 2003), and the decline in the rubisco activity is mainly due to the proteolysis of the large subunit (Hajheidari et al 2005;Jorge et al 2006). However, in agreement with our findings, Rizhsky et al (2002) reported that drought greatly increases the number of transcripts encoding the large subunit of rubisco in tobacco and suggested that the large subunit of rubisco, as a protein of the PSI reaction center, may take part in the cyclic electron flow pathway through which some energy can dissipate during stress (Rizhsky et al 2002).…”

“…The protein profiling of rice leaves subjected to drought revealed four novel droughtresponsive mechanisms (Salekdeh et al 2002a, b): upregulation of a S-like RNase homologue, an actin depolymerizing factor and a rubisco activase, and down-regulation of an isoflavone reductase-like protein (Salekdeh et al 2002a). Additionally, the response of the proteome of field-grown sugar beet leaves to drought stress has been studied (Hajheidari et al 2005).…”

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

“…Therefore, approaches involving alterations in plant plasma membrane aquaporins should, however, be viewed with prudence. Using a proteomic approach, Hajheidari et al (2005), found an osmotin-like protein up-regulated in drought conditions.…”

Research on the Rice Proteome: The Contribution of Proteomics Technology in the Creation of Abiotic Stress-Tolerant Plants

“…One way to identify potentially important drought-tolerance genes is to analyze drought-induced changes in the proteome. Hajheidari et al [18] cultivated two genotypes of sugar beet with different genetic backgrounds in the field, and they demonstrated that certain proteins in these plants show genotype-specific patterns of up-or down-regulation in response to drought. Some of these proteins may contribute a physiological advantage under conditions of drought, making them potential targets for marker-assisted selection for drought tolerance.…”

“…Drought represents one of the most severe limitations on the productivity of rain-fed lowland and upland rice. Hajheidari et al [18] reported that the efficiency of breeding for increased drought tolerance could be greatly improved by the identification of candidate genes for marker-assisted selection in sugar beet. One way to identify potentially important drought-tolerance genes is to analyze drought-induced changes in the proteome.…”