Research on the Rice Proteome: The Contribution of Proteomics Technology in the Creation of Abiotic Stress-Tolerant Plants

Abstract

Proteomics techniques have identified a vast number of proteins that participate in the growth of plants or their adaptation to environmental stresses. Functional analysis of those proteins will contribute to the development of high-yielding crops through artificial manipulation of the basic life phenomena of plants or through the assessment of their stress tolerance. The conditions in almost all farmlands are suboptimal for plant growth, resulting in a calculation that causes the loss to three quarters of the potential yield of crops. Breeding of stresstolerant cultivars has been hampered by the paucity of information on molecular events underlying stress tolerance. A comparative analysis of the response of plants to stress at the protein level, together with physiological measurements, will assist in identifying the genes and pathways that are crucial for stress tolerance. This review examines analyses of the rice proteome under abiotic stress and the potential contribution of proteomics technology in the creation of abiotic stress-tolerant plants.

Proteomics analysis of rice seedling responses to ovine saliva

“…Due to recent availability of the rice genome sequence and its protein database, the field of rice proteomics has evolved considerably in the last several years (Komastu and Yano, 2006;Komastu, 2008). The proteomics technique has facilitated numerous studies of biological processes (Zhao et al, 2005;Xie et al, 2006;Dai et al, 2007;Yin et al, 2007) and the comparative proteomics analysis under various stresses such as cold, high temperature, drought, salinity, wounding and pathogen have been reported (Rabbani et al, 2003;Shen et al, 2003;Yan et al, 2006;Chen et al, 2007;Chitteti and Peng, 2007;Lee et al, 2007;Cheng et al, 2009).…”

Proteomic dissection of the rice-Fusarium fujikuroi interaction and the correlation between the proteome and transcriptome under disease stress

“…A single protein-profiling experiment can provide only a few clues regarding the function of a protein [13]. The integration of available transcriptomic data with proteomic data is critical to add further value to the single proteomic datasets and to provide a global picture of gene regulation and metabolic networks.…”

“…During protein translation from mRNA, factors including mRNA splicing, altered protein turnover, protein degradation, or a combination of the above may lead to a poor correlation between the expression patterns of mRNAs and their respective proteins [36]. Komatsu [13] maintained that transcript and protein levels cannot be correlated, due to the inability of total mRNA to be translated into protein. The identification of proteomes that are not fully complete due to current limitations of proteomics [37] also affects global comparative studies of transcript and protein expression.…”

“…In rice breeding, proteomics is usually applied to detect stress-responsive proteins through comparisons among stressed plants. Further identification of candidate proteins may reveal that some of these proteins have functions that are clearly consistent with the stress-tolerance trait [13].…”

Plant Proteome in Response to Abiotic Stress

“…Therefore, proteomics is a powerful tool to identify genes responsible for stress tolerance. Moreover, proteome identification and physiology analysis could provide information to detect genome, stress, or term-related genes or potential biomarkers for a better description level of stress tolerance in each genotype [74].…”