Proteome Analysis of Human Placentae: Pre-eclampsia Versus Normal Pregnancy

Mine, Katsuya; Katayama, Akira; Matsumura, T.; Nishino, Tokuzo; Kuwabara, Yoshimitsu; Ishikawa, Gen; Murata, Tomoaki; Sawa, Rintaro; Otsubo, Yasuo; Shin, Sumio; Takeshita, Toshiyuki

doi:10.1016/j.placenta.2006.10.005

Cited by 31 publications

(39 citation statements)

References 33 publications

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“…Our initial examination using Western blotting showed no difference in TTR levels in the placenta of sPE patients compared to preterm controls. This is consistent with other proteomic studies that found no significant difference [11] or even upregulation of TTR [12] in placentas from PE patients. We questioned whether this might be attributed to TTR synthesized by other placental cell types or to the fetal circulation entering the placenta stroma.…”

Section: Discussionsupporting

confidence: 93%

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Preeclampsia is associated with low placental transthyretin levels

Zhu

Baczyk

Lye

et al. 2016

Taiwanese Journal of Obstetrics and Gynecology

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The immunohistochemical expression of TTR in the syncytiotrophoblast layer of the placenta decreased significantly after 12 weeks of gestation, paralleling the changing demands of thyroid hormone uptake into the placenta. The reduced TTR expression in the syncytiotrophoblast layer of the preeclamptic placenta might impair thyroid hormone uptake and contribute to the pathophysiology of the disease.

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Section: Discussionsupporting

confidence: 93%

“…Several studies compared total TTR levels between severe preeclampsia (sPE) and control placentas and found no correlation [11,12]. Recognizing TTR's role as a placental transporter, we aimed to study its spatial distribution.…”

Section: Introductionmentioning

confidence: 99%

Preeclampsia is associated with low placental transthyretin levels

Zhu

Baczyk

Lye

et al. 2016

Taiwanese Journal of Obstetrics and Gynecology

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“…All differentially overexpressed proteins (Tables 1 and 2) have been previously detected as placental tissue or placental tissue cell line-derived proteins [6, 8, 19,20,21,22]. Moreover, the expression of all proteins within normal and URPL placentas at the RNA level was also conformed in this study using the RT-PCR method.…”

Section: Discussionsupporting

confidence: 72%

Alteration in the Expression of Proteins in Unexplained Recurrent Pregnancy Loss Compared with in the Normal Placenta

2014

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The placenta is a unique pregnancy-related tissue and plays a key role in occurrence of unexplained recurrent pregnancy loss (URPL). Abnormal placentation might play a key role in occurrence of URPL. Therefore, the purpose of this study was to compare the human placental proteome between URPL placentas and normal placental matched for gestational week. Total placental proteins were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique was used for separation of the placental proteomes. Protein spots differentially expressed between URPL and normal placentas were selected and identified by the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF) technique after being digested in the gel. Moreover, quantitative real-time PCR and Western blot techniques were used to confirm the differential expression mass results for some differentially expressed proteins. The results indicated that at least 19 protein spots were differentially expressed between URPL and normal placentas (P < 0.05), and twelve of them were successfully identified. While only two proteins were downregulated (calumenin and enolase 1), the remaining ten spots (actin gamma 1 propeptide, cathepsin D prepropeptide, heat shock protein gp96, tubulin beta, tubulin alpha 1, glutathione S-transferase, vitamin D binding protein, prohibitin, actin beta, apolipoprotein A-I) showed increased expression in URPL cases in comparison with normal placentas. Real-time PCR also confirmed the downregulation of calumenin and upregulation of prohibitin and apolipoprotein A-I at the mRNA levels. In conclusion, the results of the present study showed that alteration in the expression of proteins involved in proliferation and migration of endothelial cells as well as control of coagulation by these cells might play an important role in the pathogenesis of URPL.

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“…Mass spectrometry-based proteomic approaches allow both identification and evaluation of differentially expressed protein factors, but with low sensitivity. This technique had already been used in this context on trophoblastic cells and plasma from normal and PE patients [4][5][6][7][8][9] but never on culture medium of CTB. Secreted placental proteins are circulating pregnancy associated proteins detectable in plasma of pregnant women with highly sensitive techniques such as immunological methods.…”

Section: Discussionmentioning

confidence: 99%

“…One approach that holds some promise in PE research is comparative proteomics because it allows the simultaneous detection of many proteins and peptides. Some previous studies reported a proteomic analysis of placental tissue [4][5][6][7][8][9][10]. They allowed the identification of some important proteins potentially involved in PE, such as heat shock proteins [8,10].…”

Section: Introductionmentioning

confidence: 99%