Preeclampsia is associated with low placental transthyretin levels

Zhu, Lei; Baczyk, Dora; Lye, Stephen J.; Zhang, Zhenyu

doi:10.1016/j.tjog.2016.04.014

Cited by 12 publications

(7 citation statements)

References 25 publications

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“…Negative controls were set by replacing the primary antibody with phosphate-buffered saline. The digital images were randomly taken at ×200 under light microscope (Olympus BX50, Japan) from four sections of each animal and analyzed by Image pro plus 6.0 (Media Cybemetics Inc., Silver Spring, MA, USA; Zhu et al, 2016). The data were expressed as mean density of positive area.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Epidermal Neural Crest Stem Cells on Local Inflammation Microenvironment in the Defected Sciatic Nerve of Rats

Dongdong

Liu

et al. 2017

Front. Mol. Neurosci.

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Cell-based therapy is a promising strategy for the repair of peripheral nerve injuries (PNIs). epidermal neural crest stems cells (EPI-NCSCs) are thought to be important donor cells for repairing PNI in different animal models. Following PNI, inflammatory response is important to regulate the repair process. However, the effects of EPI-NCSCs on regulation of local inflammation microenviroment have not been investigated extensively. In the present study, these effects were studied by using 10 mm defected sciatic nerve, which was bridged with 15 mm artificial nerve composed of EPI-NCSCs, extracellular matrix (ECM) and poly (lactide-co-glycolide) (PLGA). Then the expression of pro- and anti-inflammatory cytokines, polarization of macrophages, regulation of fibroblasts and shwann cells (SCs) were assessed by western blot, immunohistochemistry, immunofluorescence staining at 1, 3, 7 and 21 days after bridging. The structure and the function of the bridged nerve were determined by observation under light microscope and by examination of right lateral foot retraction time (LFRT), sciatic function index (SFI), gastrocnemius wet weight and electrophysiology at 9 weeks. After bridging with EPI-NCSCs, the expression of anti-inflammatory cytokines (IL-4 and IL-13) was increased, but decreased for pro-inflammatory cytokines (IL-6 and TNF-α) compared to the control bridging, which was consistent with increase of M2 macrophages and decrease of M1 macrophages at 7 days after transplantation. Likewise, myelin-formed SCs were significantly increased, but decreased for the activated fibroblasts in their number at 21 days. The recovery of structure and function of nerve bridged with EPI-NCSCs was significantly superior to that of DMEM. These results indicated that EPI-NCSCs could be able to regulate and provide more suitable inflammation microenvironment for the repair of defected sciatic nerve.

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Section: Methodsmentioning

confidence: 99%

The Effects of Epidermal Neural Crest Stem Cells on Local Inflammation Microenvironment in the Defected Sciatic Nerve of Rats

Dongdong

Liu

et al. 2017

Front. Mol. Neurosci.

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“…Another groups also found that maternal serum of patients suffering from severe PE has a lower level of TTR which may affect the normal development of the growing fetus 20 – 22 . In a very recent study, expression of TTR was found to be down regulated in placental tissues from severe PE patients compared to control ones 23 . These findings strongly suggest the fact that PE is associated with reduced levels of TTR.…”

Section: Introductionmentioning

confidence: 91%

MicroRNA regulation of Transthyretin in trophoblast differentiation and Intra-Uterine Growth Restriction

Saha

Chakraborty

Bhattacharya

et al. 2017

Sci Rep

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Placental trophoblast cells produce various cytokines, transporters vital to normal embryogenesis. Transthyretin (TTR) aids trans-placental passage of maternal thyroxin (TH) to fetal circulation. Inadequate TH delivery leads to developmental abnormality. Regulation of TTR biosynthesis in placenta is critical for normal embryo development. We showed here that TTR transcripts were expressed more in fetal placenta. Using bioinformatic analysis and confirmation with dual-luciferase reporter assays, we found that miR-200a-3p and miR-141-3p inhibited TTR expression by directly binding to the 3′UTR of TTR, which is reversed by mutation in the microRNA binding site. Differentiation of human trophoblast BeWo cells was associated with decreased TTR transcript and protein levels with concomitant increase in the levels of both microRNAs. Interestingly, ectopic overexpression of the microRNA mimics abrogated thyroxin uptake by BeWo cells, which was reversed by the corresponding inhibitors. Furthermore, in a rat model of intra-uterine growth restriction (IUGR), TTR expression decreased significantly in placenta with reciprocal rise in miR-141-3p but not 200a-3p. In human IUGR placenta, TTR transcript and protein levels were significantly lower associated with high expression of miR-141-3p but not 200a-3p. These data provides new insight into physiological role of miR-141-3p in regulating TTR during trophoblast differentiation and IUGR.

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“…Immunohistochemical data show that specifically on the syncytiotrophoblast layer, TTR expression peaks in the first trimester and progressively decreases throughout the second and third trimester [9]. Placental TTR expression and uptake might be enhanced by relative hypoxia observed in the first trimester of pregnancy, not coincidentally the moment when the fetus is completely dependent on maternal T4 [10].…”

Section: Maternal Thyroid Hormone Supply To the Fetusmentioning

confidence: 95%

“…Fruscalzo et al showed by immunohistochemistry that in IUGR and severe preeclampsia, there was a much stronger placental TTR signal compared to controls. They hypothesized that placental hypoxia is cause of increased TTR expression in placentas from pregnancies complicated by IUGR and preeclampsia [42], although conflicting data exist [9].…”

Section: J U S T a C C E P T E Dmentioning

confidence: 99%

The placenta in fetal thyroid hormone delivery: from normal physiology to adaptive mechanisms in complicated pregnancies

Eerdekens

Verhaeghe

Darras

et al. 2019

The Journal of Maternal-Fetal & Neonatal Medicine

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Context: Thyroid hormones are indispensable for normal fetal development. Since the fetus depends to a large extent on maternal thyroid hormone supply through the placenta, this challenges maternal thyroid economy. Several molecular mechanisms are involved in placental thyroid hormone transport and metabolism. Chronic pregnancy complications, associated with utero-placental hypoxia, trigger the development of accelerated placental maturation in order to improve fetal-placental exchange to strengthen the offspring's chance of survival. This review provides an overview of normal maternal-fetal thyroid hormone supply and explores the presence of placental adaptive mechanisms in complicated pregnancies with chronical utero-placental hypoxia to improve the thyroid hormone supply to the fetus under pressure, to end with reflections about the long term health consequences.Evidence acquisition: This work is based on a comprehensive literature review of the Pubmed and Embase database, including relevant articles from 1969 to June 2018. Conclusions:The placenta is actively involved in fetal thyroid hormone delivery through a combination of stimulatory and inhibitory mechanisms. Parallel with histological adaptations to improve trans-placental fetal-maternal exchange, there are indications of placental adaptive mechanisms in thyroid hormone transport and metabolism in case of complicated pregnancies, from animal models and in-vitro experiments. Evidence from human in-vivo studies is limited due to heterogeneity in study populations, small study samples and technical limitations. Further research is necessary to reveal the role of the placenta in pathological circumstances. The placenta might thus be considered as the infants' black box of pregnancy. Results will contribute to more insights in the concept of fetal programming, which lays the foundations of optimum health, growth, and neurodevelopment across the lifespan.

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Preeclampsia is associated with low placental transthyretin levels

Cited by 12 publications

References 25 publications

The Effects of Epidermal Neural Crest Stem Cells on Local Inflammation Microenvironment in the Defected Sciatic Nerve of Rats

The Effects of Epidermal Neural Crest Stem Cells on Local Inflammation Microenvironment in the Defected Sciatic Nerve of Rats

MicroRNA regulation of Transthyretin in trophoblast differentiation and Intra-Uterine Growth Restriction

The placenta in fetal thyroid hormone delivery: from normal physiology to adaptive mechanisms in complicated pregnancies

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