Comparative analysis of secreted proteins from normal and preeclamptic trophoblastic cells using proteomic approaches

Epiney, Manuella; Ribaux, Pascale; Arboit, Patrizia; Irion, Olivier; Cohen, Marie

doi:10.1016/j.jprot.2011.12.021

Cited by 18 publications

(18 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Secreted proteins are circulating proteins detectable in blood samples using highly sensitive techniques, including immunological methods (ELISA) (18). Although in vitro culture conditions may not fully reflect the in vivo conditions, identification of ovarian proteins differentially secreted by malignant cells may be a first step towards the elucidation of ovarian cancer markers in blood samples.…”

Section: Discussionmentioning

confidence: 99%

Comparative secretome of ovarian serous carcinoma: Gelsolin in the spotlight

et al. 2017

View full text Add to dashboard Cite

Abstract. Ovarian cancer is one of the most common types of reproductive cancer, and has the highest mortality rate amongst gynecological cancer subtypes. The majority of ovarian cancers are diagnosed at an advanced stage, resulting in a five-year survival rate of ~30%. Early diagnosis of ovarian cancer has improved the five-year survival rate to ≥90%, thus the current imperative requirement is to identify biomarkers that would allow the early detection, diagnosis and monitoring of the progression of the disease, or of novel targets for therapy. In the present study, secreted proteins from purified ovarian control, benign and cancer cells were investigated by mass spectrometry, in order to identify novel specific markers that are easy to quantify in patients sera. A total of nine proteins revealed significant differential secretion from control and benign cells, in comparison with ovarian cancer cells. The mRNA expression levels of three of these proteins (Dickkopf protein 3, heat shock protein 10 kDa and gelsolin) were subsequently evaluated by reverse transcription-quantitative polymerase chain reaction. Combined with the protein level in serum, the present study identified that gelsolin may be a useful marker of ovarian cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative secretome of ovarian serous carcinoma: Gelsolin in the spotlight

et al. 2017

View full text Add to dashboard Cite

show abstract

“…TMT mass tags labelled the intact proteins and then a fractionation process was utilised prior to mass spectroscopy analysis. LC-MS/MS was performed on a linear trap quadrupole (LTQ) Orbitrap Velos (Thermo Electron, San Jose, CA, USA) and peptide analysis was performed for identification of peptide sequences using Scaffold 3.0 (Proteome Software, Portland, OR, USA) as previously described and displayed (Epiney et al, 2012).…”

Section: G Pattappa Et Al Ccl5/rantes Is Released By Intervertebral mentioning

confidence: 99%

CCL5/RANTES is a key chemoattractant released by degenerative intervertebral discs in organ culture

Pattappa

Peroglio²,

Sakai³

et al. 2014

eCM

View full text Add to dashboard Cite

Release of chemotactic factors in response to tissue damage has been described for different musculoskeletal tissues, including the intervertebral disc (IVD). This study investigated the chemoattractants that are released by induced degenerative IVDs and may be involved in recruiting mesenchymal stem cells (MSCs). Bovine caudal discs were cultured within a bioreactor and loaded under conditions that mimicked physiological or degenerative settings. Between days 4-6, medium was replaced by PBS, which was subsequently used for proteomic, ELISA and immunoprecipitation analyses of secreted chemokines and cytokines. A Boyden chamber assay was used to observe human MSC migration towards native and chemokine depleted media. Gene expression levels of chemokine receptors in human MSCs were analysed, and CCL5 was localised in bovine and human IVD by immunohistochemistry. Proteomic analysis revealed the presence of CCL5 and CXCL6 within conditioned media. Higher concentrations of CCL5 were found in the degenerative media, and a relationship was found between interleukin-1β and CCL5 concentration. Chemokine immunoprecipitation showed that MSCs had a significantly reduced chemotactic migration towards CCL5-immunoprecipitated and CCL5/CXCL6 coimmunoprecipitated media, whilst CXCL6 depletion did not change MSC chemotaxis. MSCs showed a significant increase in mRNA expression of the CCL5 receptors, CCR1 and CCR4, upon culture in degenerative media. Furthermore, CCL5 was identified in bovine and human disc tissue by immunohistochemistry. Hence, CCL5 may be a key chemoattractant that is produced and released by the intervertebral disc cells. Therefore, these factors could be used to enhance stem/progenitor cell mobilisation in regenerative therapies for early stages of disc degeneration.

show abstract

“…Epiney et al [4] study pioneers a promising direction for proteomics by quantifying protein in cytotrophoblast culture media. While Epiney et al [4] study acknowledges that syncytial trophoblasts, not cytotrophoblast, directly exchange materials with the maternal circulation, their study begins to bridges the gap in placenta and serum proteome. This difference of cell type may explain why there is differential significance between serum studies and the cytotrophoblast sectromone.…”

Section: Discussion: Biomarkers For Preeclampsiamentioning

confidence: 99%

“…C8 and C9 are activated in the final steps to form pores on target cells and induce cell death by contributing to the membrane attack complex (MAC) [24]. Complement component 8 (C8) and complement component (C9) were observed as ambiguously regulated in two studies, one serum source reported C8 and C9 as down regulated at delivery while the other reported up-regulated but the gestational age of the samples was not reported (Table2 and Supplementary Table 1) also ambiguous was Complement factor H-1 related protein, which was previously identified as a potential biomarker for preeclampsia [4]. Additionally, Complement component 4 (C4) was ambiguous, but interestingly, C4 binding protein was observed as upregulated in Kolla's study of blood serum during week 12 of pregnancy while the same protein was observed as downregulated in Park's study of blood serum immediately before delivery.…”

Section: Immune Responsementioning

confidence: 99%

“…Preeclampsia, affecting 2-7% of all pregnancies, is a vasculopathy that results from aberrant placenta development and it is the third highest cause of pregnancy related deaths following hemorrhage and embolism [1][2][3]. While the etiology of preeclampsia is poorly understood, a leading theory is that insufficient spiral artery remodeling in the placenta may result in a hypoxic environment with reperfusion injury and up-regulation of oxidative stress [4,5]. The preeclamptic placenta secretes factors such as inflammatory cytokines and reactive oxygen species into maternal circulation to induce hypertension through local production of vasoconstrictors, notably endothelin-1 [6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation