Proteome analysis for identification of target proteins of genistein in primary human endothelial cells stressed with oxidized LDL or homocysteine

Fuchs, D.; Pascual‐Teresa, Sonia de; Rimbach, Gerald; Virgili, Fabio; Ambra, Roberto; Turner, Rufus; Daniel, Hannelore; Wenzel, U.

doi:10.1007/s00394-004-0499-6

Cited by 31 publications

(14 citation statements)

References 40 publications

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“…Grape phytoestrogens prevent cholesterol accumulation in cultured monocytes from Post-MW [165]. In HUVECs, genistein reverses homocysteine- and ox-LDL induced decrease in the anti-atherogenic proteins annexin V and lamin A [166]. Animal studies also support the anti-atherogenic properties of phytoestrogens.…”

Section: Introductionmentioning

confidence: 99%

Vascular Effects of Phytoestrogens and Alternative Menopausal Hormone Therapy in Cardiovascular Disease

Gencel¹,

Benjamin²,

Bahou³

et al. 2012

MRMC

115

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Phytoestrogens are estrogenic compounds of plant origin classified into different groups including isoflavones, lignans, coumestans and stilbenes. Isoflavones such as genistein and daidzein are the most studied and most potent phytoestrogens, and are found mainly in soy based foods. The effects of phytoestrogens are partly mediated via estrogen receptors (ERs): ERα, ERβ and possibly GPER. The interaction of phytoestrogens with ERs is thought to induce both genomic and non-genomic effects in many tissues including the vasculature. Some phytoestrogens such as genistein have additional non-ER-mediated effects involving signaling pathways such as tyrosine kinase. Experimental studies have shown beneficial effects of phytoestrogens on endothelial cells, vascular smooth muscle, and extracellular matrix. Phytoestrogens may also affect other pathophysiologic vascular processes such as lipid profile, angiogenesis, inflammation, tissue damage by reactive oxygen species, and these effects could delay the progression of atherosclerosis. As recent clinical trials showed no vascular benefits or even increased risk of cardiovascular disease (CVD) and CV events with conventional menopausal hormone therapy (MHT), phytoestrogens are being considered as alternatives to pharmacologic MHT. Epidemiological studies in the Far East population suggest that dietary intake of phytoestrogens may contribute to the decreased incidence of postmenopausal CVD and thromboembolic events. Also, the WHO-CARDIAC study supported that consumption of high soybean diet is associated with lower mortalities from coronary artery disease. However, as with estrogen, there has been some discrepancy between the experimental studies demonstrating the vascular benefits of phytoestrogens and the data from clinical trials. This is likely because the phytoestrogens clinical trials have been limited in many aspects including the number of participants enrolled, the clinical end points investigated, and the lack of long-term follow-up. Further investigation of the cellular mechanisms underlying the vascular effects of phytoestrogens and careful evaluation of the epidemiological evidence and clinical trials of their potential vascular benefits would put forward the use of phytoestrogens as an alternative MHT for the relief of menopausal symptoms and amelioration of postmenopausal CVD.

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Section: Introductionmentioning

confidence: 99%

Vascular Effects of Phytoestrogens and Alternative Menopausal Hormone Therapy in Cardiovascular Disease

Gencel¹,

Benjamin²,

Bahou³

et al. 2012

MRMC

115

View full text Add to dashboard Cite

show abstract

“…Several cell culture studies using nutritional proteomics demonstrated the effects of food components such as butyrate, flavonoid, and genistein on protein profiles [34][35][36][37][38][39]. Animal studies also showed the potential of proteomics in nutritional research.…”

Section: Proteomicsmentioning

confidence: 99%

Recent Advances in Nutraceuticals and Functional Foods

Lau¹,

Bagchi

Zafra-Stone

et al. 2010

Genomics, Proteomics, and Metabolomics in Nutraceuticals and Functional Foods

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“…In expression proteomics studies of quercetin (Wenzel et al, 2004) and genistein (Fuchs et al, 2005a), the changes in the protein expression level have revealed which downstream proteins are upregulated or downregulated. Homocysteine (Hcy) causes dysregulation of gene expression.…”

Section: Studying Molecular Targets By Expression Proteomicsmentioning

confidence: 99%

Target Identification and Polypharmacology of Nutraceuticals

Dormán¹,

Flachner²,

Hajdú³

et al. 2016

Nutraceuticals

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Proteome analysis for identification of target proteins of genistein in primary human endothelial cells stressed with oxidized LDL or homocysteine

Abstract: Proteome analysis allows the identification of potential interactions of dietary components in the molecular process of atherosclerosis and consequently provides a powerful tool to define biomarkers of response.

Cited by 31 publications

References 40 publications

Vascular Effects of Phytoestrogens and Alternative Menopausal Hormone Therapy in Cardiovascular Disease

Vascular Effects of Phytoestrogens and Alternative Menopausal Hormone Therapy in Cardiovascular Disease

Recent Advances in Nutraceuticals and Functional Foods

Target Identification and Polypharmacology of Nutraceuticals

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