Proteome alterations of reverse photoperiod-sensitive genic male sterile rice (Oryza sativa L.) at fertility transformation stage

Chen, Zhe; Li, Haixia; Ma, Xueli; Dong, Hansong; Ma, Dong; Zeng, Hanlai

doi:10.1007/s13258-014-0205-x

Cited by 5 publications

(7 citation statements)

References 69 publications

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“…Phytohormones, including auxin (AUX), GA and JA, control most developmental stages in plants. In multiple phytohormone pathways, miRNA is a regulator of mitotic cell division by regulating GROWTH RESPONDING FACTOR (GRF), TEOSINTEBRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) and NAC domain-containing proteins and thus controlling or determining the cell fate of development and senescence [ 45 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ]. In our previous research, miRNA396c- GRF1 / 3 , PCF6/7 and NAC were found to respond to temperature changes, thereby regulating PA64S male fertility [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

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Jasmonic Acid Plays a Pivotal Role in Pollen Development and Fertility Regulation in Different Types of P(T)GMS Rice Lines

Liu

Zhu

et al. 2021

IJMS

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Two-line hybrid rice systems represent a new technical approach to utilizing the advantages of rice hybrids. However, the mechanism underlying the male sterile-line fertility transition in rice remains unclear. Peiai 64S (PA64S) is a photoperiod- and thermo-sensitive genic male sterile (PTGMS) line in which male sterility manifests at an average temperature above 23.5 °C under long-day (LD) conditions. Nongken 58S (NK58S) is a LD-sensitive genic male sterile (PGMS) rice that is sterile under LD conditions (above 13.75 h-day). In contrast, D52S is a short-day (SD)-PGMS line that manifests male sterility under SD conditions (below 13.5 h-day). In this study, we obtained fertile and sterile plants from all three lines and performed transcriptome analyses on the anthers of the plants. Gene ontology (GO) analysis suggested that the differentially expressed genes identified were significantly enriched in common terms involved in the response to jasmonic acid (JA) and in JA biosynthesis. On the basis of the biochemical and molecular validation of dynamic, tissue-specific changes in JA, indole-3-acetic acid (IAA) levels, gibberellin (GA) levels, and JA biosynthetic enzyme activities and expression, we proposed that JA could play a pivotal role in viable pollen production through its initial upregulation, constant fluctuation and leaf-spikelet signaling under certain fertility-inducing conditions. Furthermore, we also sprayed methyl jasmonate (MEJA) and salicylhydroxamic acid (SHAM) on the plants, thereby achieving fertility reversal in the PGMS lines NK58S and D52S, with 12.91–63.53% pollen fertility changes. Through qPCR and enzyme activity analyses, we identified two key enzymes—allene oxide synthase (AOS) and allene oxide cyclase (AOC)—that were produced and upregulated by 20–500-fold in PGMS in response to spraying; the activities of these enzymes reversed pollen fertility by influencing the JA biosynthetic pathway. These results provide a new understanding of hormone interactions and networks in male-sterile rice based on the role of JA that will help us to better understand the potential regulatory mechanisms of fertility development in rice in the future.

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Section: Discussionmentioning

confidence: 99%

“…The genic male-sterile rice materials PA64S, D52S and NK58S were identified and bred for multiple generations by our research team [ 24 , 58 ]. PA64S is a PTGMS line derived from a cross between NK58S ( Oryza sativa L. ssp.…”

Section: Methodsmentioning

confidence: 99%

Jasmonic Acid Plays a Pivotal Role in Pollen Development and Fertility Regulation in Different Types of P(T)GMS Rice Lines

Liu

Zhu

et al. 2021

IJMS

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“…In this connection, exploring efforts for improve understanding of post-transcriptional and -translational profiles have increased in citrus. Recently, vast of metabolic pathways and biological processes, as well as included genes and proteins, were reported in male sterility cultivars or lines [ 22 , 28 – 33 ]. Among those mentioned in reports, genes or proteins have been focusing on the phenylpropanoid metabolism pathways.…”

Section: Introductionmentioning

confidence: 99%

Seedless mutant ‘Wuzi Ougan’ (Citrus suavissima Hort. ex Tanaka ‘seedless’) and the wild type were compared by iTRAQ-based quantitative proteomics and integratedly analyzed with transcriptome to improve understanding of male sterility

Zhang

et al. 2018

BMC Genet

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BackgroundBud mutation is a vital method of citrus. ‘Wuzi Ougan’ (mutant type, MT) as a bud variant of ‘Ougan’ (wild type, WT) was first found in 1996 and has become popular because of its male sterility and seedless character. Previous analysis of its cytological sections and transcriptome revealed that the abnormal microsporogenesis that occurs before the tetrad stage of anther development might be the result of down-regulated oxidation-reduction biological processes in MT. To reveal the mechanism behind the male sterility in MT at the post-transcriptional stage, proteome profiling and integrative analysis on previously obtained transcriptome and proteome data were performed in two strains.ResultsThe proteome profiling was performed by iTRAQ (isobaric Tags for relative and absolute quantitation) analysis and 6201 high-confidence proteins were identified, among which there were 487 differentially expressed proteins (DEPs) in one or more developmental stages of anthers between MT and WT. The main functional subcategories associated with the main category biological process into which the DEPs were classified were sporopollenin biosynthesis process and pollen exine formation. The enriched pathways were phenylpropanoid biosynthesis, flavonoid biosynthesis, and phenylalanine metabolism. Moreover, there were eight pathways linked in terms of being related to phenylpropanoid metabolism. Eighteen important genes related to phenylpropanoid metabolism were also analysized by qRT-PCR (quantitative real time PCR). An integrative analysis of the fold change at the transcript (log2 FPKM ratios) and protein (log1.2 iTRAQ ratios) levels was performed to reveal the consistency of gene expression at transcriptional and proteomic level. In general, the expression of genes and proteins tended to be positively correlated, in which the correlation coefficients were 0.3414 (all genes and all proteins) and 0.5686 (DEPs and according genes).ConclusionThis study is the first to offer a comprehensive understanding of the gene regulation in ‘Wuzi Ougan’ and its wild type, especially during the microsporocyte to meiosis stage. Specifically, the involved genes include those in phenylpropanoid biosynthesis, flavonoid biosynthesis, and phenylalanine metabolism, as determined by integrative transcriptome and proteome analysis.Electronic supplementary materialThe online version of this article (10.1186/s12863-018-0693-9) contains supplementary material, which is available to authorized users.

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“…Liu et al (2014) found that in photoperiod-sensitive genic male sterility cotton, altered expression of many proteins associated with energy and metabolic pathways, pollen tube growth, pollen wall development, protein metabolism, protein folding and assembly, and stress defence. Chen et al (2014) showed abundance decrease of proteins involved in lignin-flavonoid biosynthesis and energy metabolism, but up-accumulation of photosynthesis and defence-related proteins in TGMS rice. Xiao et al (2009) found the expression of some essential proteins for pollen development, mainly on energy metabolism, protein biosynthesis, and cell wall formation and stress responses changed in TGMS rice Zhu-1S.…”

Section: Discussionmentioning

confidence: 94%

“…Chen et al. () showed abundance decrease of proteins involved in lignin–flavonoid biosynthesis and energy metabolism, but up‐accumulation of photosynthesis and defence‐related proteins in TGMS rice. Xiao et al.…”

Section: Discussionmentioning

confidence: 99%

Proteomic alteration of a thermo‐sensitive male sterility SP2S in rapeseed (Brassica napus) in response to mild temperature stress

Zhang

Liu

et al. 2016

Plant Breeding

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We conducted a proteomic comparison between thermo-sensitive genic male sterility (TGMS) SP2S and its near-isogenic line SP2F grown at 22°C. The proteomes at microsporocyte meiosis and uninucleate microspore stages were profiled using 2-dimensional gel electrophoresis. Twenty-five well-reproducible spots (10 spots at microsporocyte meiosis stage and 15 spots at uninucleate microspore stage) containing 28 proteins were successfully identified by MALDI-TOF/TOF mass spectrometry. An elongation factor EF-2 at microsporocyte stage and 4 proteins (aconitate hydratase, triosephosphate isomerase, serine/arginine-rich mRNA splicing factor and glutathione S-transferase) at uninucleate microspore stage accumulated in SP2S, but more proteins were lost or reduced, including those associated with amino acid metabolism, photosynthesis, synthesis and degradation of protein, lipid metabolism, cytoskeleton, RNA modification, oxidoreductase and defence response. The dramatic decrease of tubulin, actin and Translationally controlled tumour protein (TCTP) crucial for microtubule and cell division and three enzymes for amino acid metabolite at early stage indicated a serious defect on the cytokinesis. They were important clues for us to search the TGMS genes and its interacting genes.

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Proteome alterations of reverse photoperiod-sensitive genic male sterile rice (Oryza sativa L.) at fertility transformation stage

Cited by 5 publications

References 69 publications

Jasmonic Acid Plays a Pivotal Role in Pollen Development and Fertility Regulation in Different Types of P(T)GMS Rice Lines

Jasmonic Acid Plays a Pivotal Role in Pollen Development and Fertility Regulation in Different Types of P(T)GMS Rice Lines

Seedless mutant ‘Wuzi Ougan’ (Citrus suavissima Hort. ex Tanaka ‘seedless’) and the wild type were compared by iTRAQ-based quantitative proteomics and integratedly analyzed with transcriptome to improve understanding of male sterility

Proteomic alteration of a thermo‐sensitive male sterility SP2S in rapeseed (Brassica napus) in response to mild temperature stress

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