Proteomic alteration of a thermo‐sensitive male sterility SP2S in rapeseed (Brassica napus) in response to mild temperature stress

Abstract

We conducted a proteomic comparison between thermo-sensitive genic male sterility (TGMS) SP2S and its near-isogenic line SP2F grown at 22°C. The proteomes at microsporocyte meiosis and uninucleate microspore stages were profiled using 2-dimensional gel electrophoresis. Twenty-five well-reproducible spots (10 spots at microsporocyte meiosis stage and 15 spots at uninucleate microspore stage) containing 28 proteins were successfully identified by MALDI-TOF/TOF mass spectrometry. An elongation factor EF-2 at microsporocyte stage and 4 proteins (aconitate hydratase, triosephosphate isomerase, serine/arginine-rich mRNA splicing factor and glutathione S-transferase) at uninucleate microspore stage accumulated in SP2S, but more proteins were lost or reduced, including those associated with amino acid metabolism, photosynthesis, synthesis and degradation of protein, lipid metabolism, cytoskeleton, RNA modification, oxidoreductase and defence response. The dramatic decrease of tubulin, actin and Translationally controlled tumour protein (TCTP) crucial for microtubule and cell division and three enzymes for amino acid metabolite at early stage indicated a serious defect on the cytokinesis. They were important clues for us to search the TGMS genes and its interacting genes.

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

“…In various protein separation methods, 2-DE is most promising and accepted as a separation method and an analytical approach because of its powerful separation ability. 166 For example, 2-DE was used to decipher the molecular networks regulating the salt stress in rapeseed. A salinity susceptible cultivar of rapeseed was subjected to different salt treatments and found that Na + content and proline concentration were enhanced in leaf.…”

De Novo Transcriptome Reconstruction of a Thermo‐Sensitive Male Sterility Mutant in Rapeseed (Brassica napus; Brassicaceae)

“…Some structural abnormalities in SP2S were observed, including the premature breakdown of an extremely vacuolated tapetum and a delayed dissolution of the tetrad wall covering the microspore ( Yu et al, 2015 ). We attempted to find some molecular clues to the TGMS system using a proteomic comparison of the floral buds of SP2S with its near-isogenic line (NIL) SP2F, but we identified only 28 differentially accumulated peptides ( Zhang et al, 2016 ). Transcriptomic analysis of a physiological process may obtain additional information that differs from proteomic analysis due to the very high resolution of transcriptome profiling and a limited correspondence between the mRNA level and protein abundance ( Kubala et al, 2015 ).…”