Proteoglycans of Bovine Cementum: Isolation and Characterization

Bartold, P. Mark; Reinboth, Betty; Nakae, Hajime; Narayanan, A. Sampath; Page, Roy C.

doi:10.1016/s0934-8832(11)80132-0

Cited by 46 publications

(26 citation statements)

References 31 publications

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“…The PGs act as hydrophilic monomers in association with other more or less hydrophilic polymers such as collagen, in both hard and soft tissues. To date, works by Bartold et al, 21,22 Cheng et al, 23 and Yamamoto et al, 24 confirmed the existence of PGs within bulk cementum as well as within the zone that connects cementum to root dentin. Cementum contains various glycosaminoglycans including chondroitin sulfate and probably small amounts of hyaluronan and dermatan sulfate.…”

Section: Discussionmentioning

confidence: 89%

“…21 Yamamoto et al, 24 using enzymes such as hyaluronidase and trypsin, concluded that PGs were localized within a narrow zone of 1-3 m in thickness, which they termed the CDJ. Bartold et al 22 have shown that these molecules have a larger distribution by demonstrating chondroitin sulfate PGs in the pericellular spaces within the lacunae and in the extracellular spaces of cementum. Localization of lumincan and fibromodulin PGs in precementum walls of the lacunae and cementocytes have recently been shown in bovine bulk cementum by Cheng et al 23 The existence of various PGs within cementum is convincing, but the existence of PGs within a limited width of 1-3 m between cementum and dentin needs more investigation.…”

Section: Discussionmentioning

confidence: 99%

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Ultrastructure and nanomechanical properties of cementum dentin junction

Balooch

Goodis

et al. 2003

J Biomedical Materials Res

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The attachment between cementum and dentin has been given several definitions and nomenclature, including: interzonal layer, intermediate cementum, collagen hiatus, Hopewell-Smith's hyaline layer, and more commonly, cementum-dentin junction (CDJ). Understanding the attachment of two structurally dissimilar hard tissues such as cementum and dentin defined by a junction may provide information necessary to engineer functionally graded materials that can be used for efficient tooth restorations in clinical dentistry and other bioengineering applications. Hence, in this study, as a first step toward understanding the CDJ using a biomechanical approach, it was hypothesized that the CDJ between cementum and dentin is a wide zone with mechanical properties significantly lower than the neighboring tissues. The structure of the CDJ was studied using an atomic force microscope (AFM), and site-specific mechanical response of the three regions; cementum, CDJ, and dentin were determined using an AFM-nanoindenter under dry and wet conditions. The AFM results of the CDJ demonstrated a valley under dry conditions and a peak under wet conditions. The magnitude of the depth of the valley was approximately the same as the height of the peak of the CDJ, ranging from 10 to 40 microm. The nanomechanical properties under dry conditions indicated no significant difference (p > 0.05) in elastic modulus and hardness of the CDJ (Er = 17.5 +/- 2.7 GPa, H = 0.6 +/- 0.1 GPa) and cementum (Er = 18.7 +/- 2.5 GPa, H = 0.6 +/- 0.1 GPa). The mechanical properties of the CDJ were significantly lower (p << 0.05) than dentin (Er = 19.9 +/- 2.9 GPa, H = 0.6 +/- 0.1 GPa) under dry conditions. However, under more relevant hydrated conditions, the mechanical properties of CDJ (Er 3.0 +/- 0.7 GPa, H = 0.1 +/- 0.0 GPa) were significantly lower (p << 0.05) than those of cementum (Er 6.8 +/- 1.9 GPa, H = 0.2 +/- 0.1 GPa) and dentin (Er 9.4 +/- 2.3 GPa, H = 0.3 +/- 0.1 GPa). Based on the results from this study, it can be concluded that the CDJ can be regarded as a wide zone containing large quantities of proteins including collagen that contribute to hydration and significantly reduce mechanical properties, compared with the adjacent hard tissues, cementum, and dentin. The lower mechanical properties of the CDJ may make it possible for it to redistribute occlusal loads to the alveolar bone.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Ultrastructure and nanomechanical properties of cementum dentin junction

Balooch

Goodis

et al. 2003

J Biomedical Materials Res

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show abstract

“…Although it has been shown that cementum contains mostly type I collagen (Birkedal-Hansen et al, 1977;Christner et al, 1977;Tung et al, 1985), with lesser amounts of various other collagen types, much less is known about the noncollagenous organic moieties in this tissue. Earlier reports (Chovelon et al, 1975;Smith et al, 1983) described cementum as containing a larger proportion of NCPs than found in bone or dentin and that this material may, to a large extent, be comprised of multiple classes of proteoglycans and glycosaminoglycans (Vidal et al, 1974;Bartold et al, 1988Bartold et al, , 1990. Other studies reported the identification of phosphopro- teins in cementum or tooth roots (Glimcher and Lefteriou, 1989;McCurdy et al, 1990McCurdy et al, , 1992.…”

Section: Organic Composition Of Cementummentioning

confidence: 99%

Extracellular matrix in tooth cementum and mantle dentin: Localization of osteopontin and other noncollagenous proteins, plasma proteins, and glycoconjugates by electron microscopy

1996

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“…Table 1 enumerates the types of PGs and GAGs identified within dental tissues by numerous investigators. The presence of chondroitin sulfate rich PGs such as versican, biglycan, and decorin within bovine bulk cementum was illustrated by Bartold et al (1990), and Cheng et al (1999). Bartold et al (1990), showed that these molecules have a larger distribution by demonstrating chondroitin sulfate PGs in the pericellular spaces within the lacunae and in the extracellular spaces of cementum.…”

Section: Introductionmentioning

confidence: 99%

“…The presence of chondroitin sulfate rich PGs such as versican, biglycan, and decorin within bovine bulk cementum was illustrated by Bartold et al (1990), and Cheng et al (1999). Bartold et al (1990), showed that these molecules have a larger distribution by demonstrating chondroitin sulfate PGs in the pericellular spaces within the lacunae and in the extracellular spaces of cementum. Localization of lumnican and fibromodulin PGs in pre-cementum walls of the lacunae and cementocytes has recently been shown in bovine bulk cementum by Cheng et al (1999).…”

Section: Introductionmentioning

confidence: 99%