PROTEKSI VAKSIN MONOVALEN DAN KOKTAIL SEL UTUH TERHADAP KO-INFEKSI Mycobacterium fortuitum DAN Aeromonas hydrophila PADA IKAN GURAME, Osphronemus gouramy Uni

Purwaningsih, Uni; Indrawati, Agustin; Lusiastuti, Angela Mariana

doi:10.15578/jra.9.2.2014.283-294

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…Vaksin bivalen dibuat menggunakan komposisi terbaik dengan mencampurkan masing-masing sediaan vaksin bakteri inaktif A. hydrophila 10 11 cfu dan M. fortuitum 10 10 cfu dengan perbandingan 1:1 (v/v) (Sugiani et al, 2013;Purwaningsih et al, 2014).…”

Section: Vaksin Bivalenunclassified

EFEKTIVITAS VAKSIN BIVALEN Aeromonas hydrophila DAN Mycobacterium fortuitum UNTUK PENCEGAHAN INFEKSI PENYAKIT PADA IKAN GURAMI (Osphronemus goramy)

Sugiani

Aryati

Mufidah

et al. 2015

Jurnal Riset Akuakultur

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ABSTRAKVaksinasi merupakan salah satu cara terbaik untuk pencegahan terhadap penyakit ikan. Aplikasi pemberian vaksin untuk skala massal oleh pembudidaya ikan masih menjadi kendala. Tujuan dari penelitian ini adalah untuk menguji efektivitas dan respons peningkatan kekebalan tubuh ikan terhadap penggunaan vaksin bivalen anti A. hydrophila dan M. fortuitum pada penanggulangan kejadian ko-infeksi A. hydrophila dan M. fortuitum pada ikan gurami. Perlakuan vaksinasi pada ikan gurami dilakukan selama empat minggu pemeliharan menggunakan tiga metode yang berbeda yaitu melalui injeksi, perendaman, dan oral, setiap perlakuan diulang sebanyak tiga kali dan diuji tantang selama empat minggu dengan ko-infeksi dari A. hydrophila dan M. fortuitum. Nilai RPS (relative percent survival) terbaik setelah uji tantang untuk penggunaan vaksin bivalen melalui perendaman pada benih ikan gurami adalah sebesar 26,4% lebih rendah dibandingkan dengan metode injeksi (56,33%). Vaksin bivalen kurang efektif jika diberikan melalui oral dengan nilai RPS 13,6%. Dosis vaksin bivalen terbaik untuk perlakuan vaksinasi melalui injeksi, perendaman, dan oral adalah campuran antara bakteri A. hydrophila 10 PENDAHULUANVaksinasi ikan untuk melawan berbagai infeksi bakteri patogen secara serempak dapat dilakukan dengan menggunakan vaksin bivalen (vaksin yang mengandung dua jenis patogen) atau polivalen (vaksin yang mengandung beberapa jenis patogen). Strategi vaksinasi yang ideal perlu memenuhi beberapa kriteria berikut ini, penyakit spesifik yang akan dipapar, jenis vaksin, metode vaksinasi, pemilihan waktu vaksinasi, dan perlakuan vaksinasi ulang (booster). Vaksin polivalen harus mampu melindungi dari semua serotipe dari tiap patogen penyebab penyakit tertentu. Namun, kemungkinan adanya kompetisi antigen spesifik yang mungkin terjadi terutama ketika vaksin diaplikasikan melalui injeksi perlu diperhatikan (Toranzo et al., 2009).

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Section: Vaksin Bivalenunclassified

EFEKTIVITAS VAKSIN BIVALEN Aeromonas hydrophila DAN Mycobacterium fortuitum UNTUK PENCEGAHAN INFEKSI PENYAKIT PADA IKAN GURAMI (Osphronemus goramy)

Sugiani

Aryati

Mufidah

et al. 2015

Jurnal Riset Akuakultur

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“…The main disease in gourami farming is Motile Aeromonad Septicemia (MAS) caused by Aeromonas hydrophila bacteria (Novita et al, 2021). As reported by Purwaningsih et al (2014), MAS in addition to causing death, also has an impact on decreasing the quality of fish produced. The mass death of 47 tons of gourami occurred in West Sumatra in 2006 (Department of Marine Affairs and Fisheries, Padang Pariaman Regency, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, a number of efforts were made to overcome the problem of disease in gourami, both through a vaccination approach (Purwaningsih et al, 2014), feed (Farisi et al, 2020), immunostimulants (Wahjuningrum et al, 2010), and probiotics (Novian et al, 2023). Disease control in gourami through genetic approaches has not been reported, although such approaches have been widely applied to a number of important fish commodities such as tilapia (Zhou et al, 2013), and goldfish (Hayuningtyas et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of potential molecular markers for disease resistance in giant gourami through major histocompatibility complex (MHC) II gene analysis

Khasani,

Febrianti,

Sularto

et al. 2024

Fish Aquat Sci

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Research to obtain molecular markers related to the major histocompatibility complex (MHC) gene in both strains of gourami is essential to increase the success of the selection program of disease resistance traits. Using a completely randomized design (CRD), the challenge test consists of four treatments and seven replications. The treatment was Jambi gourami injected with PBS (KJ), Kalimantan gourami injected with PBS (KK), Jambi strain injected with Aeromonas hydrophila (GJ), and Kalimantan strain injected with A. hydrophila (GK). The GJ population was more resistant to A. hydrophila than the GK population. The MHC II gene was detected in both test strains (GJ and GK), both resistant and susceptible fish. However, there were differences in the results of amplifying the MHC II gene in susceptible and resistant fish. Two DNA fragments approximately 400 and 585 bp were detected in the genome of susceptible fish, while in the genome of susceptible fish, only one DNA fragment was detected (400 bp). Therefore, the MHC II gene fragment with a size of about 585 bp can be used as a potential candidate for specific molecular markers to obtain resistance to A. hydrophila bacteria in the giant gourami.

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“…Aeromonas hydrophila bacteria causes Motile Aeromonad Septicemia (MAS) disease in gourami (Öztürk and Altinok 2014;Kusdarwati et al 2018). Aeromonas hydrophila bacterial infection causes death and reduces the quality of the harvested fish (Purwaningsih et al 2014). MAS disease is reported to cause mass mortality of cultured gourami in Indonesia.…”

Section: Introductionmentioning

confidence: 99%

Assessing the susceptibility of the selected gourami (Osphronemus goramy) to Aeromonas hydrophila

2021

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Abstract. Febrianti R, Khasani I, Rosada KK. 2021. Assessing the susceptibility of the selected gourami (Osphronemus goramy) to Aeromonas hydrophila. Nusantara Bioscience 13: 111-120. A breeding program to improve the growth performance of the gourami fish was carried out through selection methods that produced faster growth gourami (selected population). The purpose of this study was to determine the susceptibility of the selected gourami to Aeromonas hydrophila infection based on tolerance limits (LD50) and investigated clinical signs post-injection of the pathogenic bacteria. The challenge test by intramuscular injection of A. hydrophila was done to the fingerling fish (15-20 g) for 14 days post-infection. The population of the tested fish was obtained from six families, selected gourami (SP), and non-selected control (CP) groups. Phosphate buffered saline (PBS) as control and several doses of the pathogen, 102, 104, 105, 106, 107, and 108 CFU/mL of A. hydrophila, were injected into the fish. Fish mortality and clinical signs were observed daily. The fish mortality was confirmed by isolating bacteria in the fish which showed clinical signs, followed by biochemical characterization of the isolated bacteria using API 20E and PCR. The LD50 of A. hydrophila to the selected population (9.70 × 105 CFU/mL) was higher than that of the control (6.50 × 104 CFU/mL). On the final day of the test, the accumulation mortality of CP (63.33±5.77%) higher than that in SP (33.33±5.77%). Based on the output test statistics, it was known that there was a significant difference between the mortality and clinical signs of SP and CP. The data suggested that the selected gourami were more resistant to A. hydrophila infection than that of the control. The A. hydrophila infection caused most of the major clinical signs, including mass mortality of the fish. The biochemical and PCR test ensure that fish mortality was caused by A. hydrophila infection.

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PROTEKSI VAKSIN MONOVALEN DAN KOKTAIL SEL UTUH TERHADAP KO-INFEKSI Mycobacterium fortuitum DAN Aeromonas hydrophila PADA IKAN GURAME, Osphronemus gouramy Uni

Cited by 4 publications

References 8 publications

EFEKTIVITAS VAKSIN BIVALEN Aeromonas hydrophila DAN Mycobacterium fortuitum UNTUK PENCEGAHAN INFEKSI PENYAKIT PADA IKAN GURAMI (Osphronemus goramy)

EFEKTIVITAS VAKSIN BIVALEN Aeromonas hydrophila DAN Mycobacterium fortuitum UNTUK PENCEGAHAN INFEKSI PENYAKIT PADA IKAN GURAMI (Osphronemus goramy)

Identification of potential molecular markers for disease resistance in giant gourami through major histocompatibility complex (MHC) II gene analysis

Assessing the susceptibility of the selected gourami (Osphronemus goramy) to Aeromonas hydrophila

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