Proteins Identified from Saliva and Salivary Glands of the Chinese Gall Aphid <i>Schlechtendalia chinensis</i>

Yang, Zixiang; Ma, Lin; Francis, Frédéric; Yang, Ying; Chen, Hang; Wu, Haixia; Xiao-ming, Chen

doi:10.1002/pmic.201700378

Cited by 22 publications

(25 citation statements)

References 17 publications

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“…Significant identification of 369 proteins was obtained with at least two unique peptides. In Schlechtendalia chinensis, 141 proteins were obtained from SG by an LC-MS/MS analysis (Yang et al, 2018). A similar result was found in the SG proteomes of Nezara viridula and Halyomorpha halys, identifying 305 and 238 proteins, respectively (Serteyn and Francis, 2019).…”

Section: Discussionsupporting

confidence: 56%

A Transcriptomic and Proteomic Analysis of the Diaphorina citri Salivary Glands Reveals Genes Responding to Candidatus Liberibacter asiaticus

et al. 2020

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The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is the principal vector of the Candidatus Liberibacter asiaticus (CLas) bacterium that causes Huanglongbing (HLB) disease. The D. citri salivary glands (SG) is an important barrier to the transmission of CLas. Despite its importance, the transcriptome and proteome of SG defense against CLas are unstudied in D. citri. In the present study, we generated a comparative transcriptome dataset of the SG in infected and uninfected D. citri using an Illumina RNA-Seq technology. We obtained 407 differentially expressed genes (DEGs), including 159 upregulated DEGs and 248 downregulated DEGs. Functional categories showed that many DEGs were associated with the ribosome, the insecticide resistance, the immune response and the digestion in comparison with CLas-infected SG and CLasfree SG. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases confirmed that metabolism and immunity were important functions in the SG. Among the DEGs, 68 genes (35 upregulated and 33 downregulated) encoding putativesecreted proteins were obtained with a signal peptide, suggesting that these genes may play important roles in CLas infection. A total of 673 SG proteins were identified in uninfected D. citri by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis, and 30 DEGs (15 upregulated and 15 downregulated) were found using the local tBLASTP programs. Among the 30 DEGs, many DEGs mainly involved in the metabolism and cellular processes pathways. This study provides basic transcriptome and proteome information for the SG in D. citri, and helps illuminate the molecular interactions between CLas and D. citri.

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Section: Discussionsupporting

confidence: 56%

A Transcriptomic and Proteomic Analysis of the Diaphorina citri Salivary Glands Reveals Genes Responding to Candidatus Liberibacter asiaticus

et al. 2020

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“…Color areas indicate proteins were detected in the aphid species. Ac, Aphis craccivora (Loudit et al, 2018); Ap, Acyrthosiphon pisum (Boulain et al, 2018); Dn, Diuraphis noxia (Nicholson et al, 2012); Mc, Myzus cerasi (Thorpe et al, 2016); Me, Macrosiphum euphorbiae (Chaudhary et al, 2015); Mp, Myzus persicae (Thorpe et al, 2016); Rp, Rhopalosiphum padi (Escudero-Martinez et al, 2020); Sc, Schlectendalia chinensis (Yang et al, 2018); Sg, Schizaphis graminum (Nicholson & Puterka, 2014)…”

Section: Conflict Of Interestsmentioning

confidence: 99%

Insight into watery saliva proteomes of the grain aphid, Sitobion avenae

Zhang

Francis

et al. 2020

Arch Insect Biochem Physiol

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The grain aphid, Sitobion avenae, is an economically important cereal pest worldwide. Aphid saliva plays an essential role in the interaction between aphids and their host plants. However, limited information is available regarding the proteins found in the saliva of S. avenae. Here, the watery saliva proteins from S. avenae were collected in an artificial diet and identified using a liquid chromatography-mass spectrometry/mass spectrometry analysis. A total of 114 proteins were identified in S. avenae saliva, including several enzymes, binding proteins, and putative effectors, as well as other proteins with unknown functions. In comparison with salivary proteins from nine other aphid species, the most striking feature of the salivary protein from S. avenae was the different patterns of protein functions. Several orthologous proteins secreted by other aphid species such as glucose dehydrogenase, elongation factors, and effector C002 were also detected in S. avenae saliva and speculated to play a significant role in aphid-plant interactions. These results provide further insight into the molecular basis between aphids and cereal plant interactions.

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“…S. chinensis feeds on host leaves where it injects saliva into the host leaf cells, resulting in gall formation. A total of 141 proteins have been identified from its salivary glands by LC-MS/MS analysis (Yang et al, 2018). In comparison to salivary proteins from 10 other free-living Hemipterans, the presence of a high proportion of proteins with binding activity was noticeable, including DNA-, protein-, ATP-, and iron-binding proteins, which may all be involved in gall formation.…”

Section: Resultsmentioning

confidence: 99%

“…A total of 141 proteins have been identified from saliva and dissected salivary glands of S. chinensis in a previous study (Yang et al, 2018). Genes coding these 141 proteins were presented in the newly annotated S. chinensis genome of this study.…”

Section: Methodsmentioning

confidence: 99%

Chromosome-level genome assembly of the horned-gall aphid,Schlechtendalia chinensis(Hemiptera: Aphididae: Erisomatinae)

Wei

Huang

et al. 2021

Preprint

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The horned gall aphid Schlechtendalia chinensis, is an economically important insect that induces galls valuable for medicinal and chemical industries. S. chinensis manipulates its host plant to form well-organized horned galls during feeding. So far, more than twenty aphid genomes have been reported; however, all of those are derived from free-living aphids. Here we generated a high-quality genome assembly of S. chinensis, representing the first genome sequence of a galling aphid. The final genome assembly was 280.43 Mb, with 97% of the assembled sequences anchored into thirteen chromosomes. S. chinensis presents the smallest aphid genome size among available aphid genomes to date. The contig and scaffold N50 values were 3.11 Mb and 20.58 Mb, respectively. The assembly included 96.4% of conserved arthropod and 97.8% of conserved Hemiptera single-copy orthologous genes based on BUSCO analysis. A total of 13,437 protein-coding genes were predicted. Phylogenomic analysis showed that S. chinensis formed a single clade between the Eriosoma lanigerum clade and the Aphidini+Macrosiphini aphid clades. In addition, salivary proteins were found to be differentially expressed when S. chinensis underwent host alternation, indicating their potential roles in gall formation and plant defense suppression. A total of 36 cytochrome P450 genes were identified in S. chinensis, considerably fewer compared to other aphids, probably due to its small host plant range. The high-quality S. chinensis genome assembly and annotation provide an essential genetic background for future studies to reveal the mechanism of gall formation and to explore the interaction between aphids and their host plants.

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Proteins Identified from Saliva and Salivary Glands of the Chinese Gall Aphid Schlechtendalia chinensis

Cited by 22 publications

References 17 publications

A Transcriptomic and Proteomic Analysis of the Diaphorina citri Salivary Glands Reveals Genes Responding to Candidatus Liberibacter asiaticus

A Transcriptomic and Proteomic Analysis of the Diaphorina citri Salivary Glands Reveals Genes Responding to Candidatus Liberibacter asiaticus

Insight into watery saliva proteomes of the grain aphid, Sitobion avenae

Chromosome-level genome assembly of the horned-gall aphid,Schlechtendalia chinensis(Hemiptera: Aphididae: Erisomatinae)

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