Insight into watery saliva proteomes of the grain aphid, Sitobion avenae

Abstract

The grain aphid, Sitobion avenae, is an economically important cereal pest worldwide. Aphid saliva plays an essential role in the interaction between aphids and their host plants. However, limited information is available regarding the proteins found in the saliva of S. avenae. Here, the watery saliva proteins from S. avenae were collected in an artificial diet and identified using a liquid chromatography-mass spectrometry/mass spectrometry analysis. A total of 114 proteins were identified in S. avenae saliva, including several enzymes, binding proteins, and putative effectors, as well as other proteins with unknown functions. In comparison with salivary proteins from nine other aphid species, the most striking feature of the salivary protein from S. avenae was the different patterns of protein functions. Several orthologous proteins secreted by other aphid species such as glucose dehydrogenase, elongation factors, and effector C002 were also detected in S. avenae saliva and speculated to play a significant role in aphid-plant interactions. These results provide further insight into the molecular basis between aphids and cereal plant interactions.

Activation of Wheat Defense Response by Buchnera aphidicola-Derived Small Chaperone Protein GroES in Wheat Aphid Saliva

“…Wheat aphids were fed on sterile water with 20% ( m / v ) sugar sandwiched between two layers of the parafilm membrane at one end of a glass cylinder. , Watery saliva was collected from around 20,000 aphids. Mass spectrometry (MS) analysis was performed as described previously .…”

“…Wheat aphids were fed on sterile water with 20% ( m / v ) sugar sandwiched between two layers of the parafilm membrane at one end of a glass cylinder. , Watery saliva was collected from around 20,000 aphids. Mass spectrometry (MS) analysis was performed as described previously . A search of the corresponding database (Uniprot Acyrthosiphon pisum and Sitobion avenae salivary gland transcriptomic database, accession number SRR11476040) was performed based on the raw data obtained from MS, and protein identification was performed based on the results of the database search.…”

“…Mass spectrometry (MS) analysis was performed as described previously. 25 A search of the corresponding database (Uniprot Acyrthosiphon pisum and Sitobion avenae salivary gland transcriptomic database, accession number SRR11476040) was performed based on the raw data obtained from MS, and protein identification was performed based on the results of the database search. The quality of the MS data was evaluated by analyzing the mass tolerance distribution of peptides, proteins, and parent ions.…”

Transcriptomic and proteomic analyses provide insights into host adaptation of a bamboo-feeding aphid

“…Characterization of salivary components is crucial for understanding adaptation of aphids to specific host plants. Salivary proteins are generally identified by transcriptomic and/or proteomic analyses of the dissected salivary glands and/or secreted saliva ( Nicholson et al., 2012 ; Atamian et al., 2013 ; van Bel and Will, 2016 ; Zhang et al., 2017 ; Dommel et al., 2020 ; Zhang et al., 2020 ). Integrated transcriptomic and proteomic analyses of salivary protein composition can help identify new salivary protein, and obtain a more accurate and comprehensive salivary protein repertoire.…”

Salivary Effector Sm9723 of Grain Aphid Sitobion miscanthi Suppresses Plant Defense and Is Essential for Aphid Survival on Wheat

“…Infestation of S. miscanthi can upregulate expression levels of many defense response genes in wheat [28,29] and the watery saliva secreted by S. miscanthi can induce aphid resistance in wheat [30]. In addition, transcriptomic analysis of S. miscanthi salivary glands identified 526 transcripts predicted to encode secretory proteins and 114 salivary proteins were identified in the watery saliva of S. miscanthi using tandem mass spectrometry [31,32]. However, few studies can identify the role of these salivary proteins in interactions between S. miscanthi and wheat.…”