A Transcriptomic and Proteomic Analysis of the Diaphorina citri Salivary Glands Reveals Genes Responding to Candidatus Liberibacter asiaticus

Liu, Xiaoqiang; Jiang, Hong‐Bo; Liu, Tianyuan; Yang, Li; Fan, Jiaqi; Xiong, Ying; Jing, Tian‐Xing; Lou, Binghai; Dou, Wei; Wang, Jinjun

doi:10.3389/fphys.2020.582505

Cited by 11 publications

(10 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared with other honeydew‐secreting hemipteran species, D. citri had more aquaporin genes, but it had fewer alpha‐amylase and sugar transporter genes than A. pisum and B. tabaci (Table S15). As the C Las transmission vector, studies on the colonization and propagation mechanism of pathogenic bacteria in the salivary gland and midgut of D. citri are important for controlling HLB (Santos‐Ortega & Killiny, 2018; Liu et al ., 2020). It remains to be explored regarding how the secreted proteins in the salivary gland interact with C Las.…”

Section: Resultsmentioning

confidence: 99%

Chromosome‐level genome assembly of the Asian citrus psyllid, Diaphorina citri

Liu

Pan

et al. 2023

Insect Science

View full text Add to dashboard Cite

Diaphorina citri is a global citrus pest. As a vector insect, it can transmit the causative agents of citrus huanglongbing, causing irreversible losses to the citrus industry. The acquisition of genomic information can provide a molecular genetic basis for effective control of D. citri. Here, the DNBSEQ™, Oxford Nanopore Technologies, and Hi‐C technologies are applied to generate a high‐quality chromosome‐level genome of D. citri. The genome size of D. citri was 523.78 Mb with a scaffold N50 of 47.05 Mb distributed on 13 chromosomes. A total of 250.64 Mb (47.85%) repeat sequences and 24 048 protein‐coding genes were predicted. Genome resequencing of female and male individuals indicated that the sex chromosome system of D. citri is XO. Phylogenetic analysis demonstrated that D. citri and Pachypsylla venusta, which separated from their most recent common ancestor about 336.62 million years ago, were the most closely related. Additionally, we identified genes potentially involved in detoxification metabolism, pathogen transmission, and honeydew secretion for further investigation. The high‐quality genome provides an important reference for developing effective management strategies of D. citri.

show abstract

Section: Resultsmentioning

confidence: 99%

Chromosome‐level genome assembly of the Asian citrus psyllid, Diaphorina citri

Liu

Pan

et al. 2023

Insect Science

View full text Add to dashboard Cite

show abstract

“…Surprisingly, most of the proteases detected in C. punctata saliva were cysteine-type C1 family peptidases. Salivary cysteine proteases have been found primarily in phytophagous hemipterans, where they digest plant-derived proteins and play a role in immunity against bacterial pathogens [83][84][85][86]. In addition, salivary cathepsins from aphids elicit plant defences during feeding [87].…”

Section: Discussionmentioning

confidence: 99%

You are what you eat—ecological niche and microhabitat influence venom activity and composition in aquatic bugs

et al. 2023

View full text Add to dashboard Cite

True water bugs (Nepomorpha) are mostly predacious insects that live in aquatic habitats. They use their piercing–sucking mouthparts to inject venomous saliva that facilitates the capture and extra-oral digestion of prey animals, but their venom can also be deployed for defence. In Central Europe, nepomorph species representing different families coexist in the same habitat. However, their feeding ecology, including venom composition and deployment, has not been investigated in detail. We used an integrated proteotranscriptomic and bioactivity-based approach to test whether venom composition and activity differ between four water bug species sharing the same habitat but occupying different ecological niches. We found considerable species-dependent differences in the composition of digestive enzymes and venom components that probably evolved as adaptations to particular food sources, foraging strategies and/or microhabitats. The venom of Corixa punctata differed substantially from that of the three strictly predatory species ( Ilyocoris cimicoides , Notonecta glauca and Nepa cinerea ), and the abundance of herbivory-associated proteins confirms a mostly plant-based diet. Our findings reveal independent adaptations of the digestive and defensive enzyme repertoires accompanied by the evolution of distinct feeding strategies in aquatic bugs.

show abstract

“…The Asian citrus psyllid Diaphorina citri Kuwayama is a primary pest of citrus due to its transmission of the pathogen causing Huanglongbing, a devastating and uncurable citrus disease [ 21 ]. Although transcriptomic and proteomic analyses have analyzed D. citri salivary gland gene expression and salivary secretions [ 13 , 22 , 23 ], the role of saliva in the interaction between D. citri and host plants has not been investigated. In citrus groves, D. citri are sympatric with other herbivorous insects, including citrus aphids [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Saliva-Mediated Contrasting Effects of Two Citrus Aphid Species on Asian Citrus Psyllid Feeding Behavior and Plant Jasmonic Acid Pathway

Gao

Tao

Arthurs

et al. 2023

Insects

View full text Add to dashboard Cite

While herbivorous insect saliva plays a crucial role in the interaction between plants and insects, its role in the inter-specific interactions between herbivorous insects has received little attention. Pre-infestation of citrus plants with Aphis spiraecola Patch and Aphis (Toxoptera) citricidus (Kirkaldy) exhibited positive and negative effects on the performance (feeding and reproduction) of Diaphorina citri Kuwayama. We explored the role of saliva in this plant-mediated interaction by infiltrating fresh and boiled aphid saliva into plants and detecting D. citri feeding behavior and citrus plant defense response. Leaf infiltration of A. spiraecola saliva disrupted the subsequent feeding of D. citri, indicated by prolonged extracellular stylet pathway duration and decreased phloem sap ingestion duration. By contrast, infiltration of A. citricidus saliva decreased the duration of the extracellular stylet pathway and phloem sap ingestion of D. citri. Furthermore, gene expression analysis showed that several salicylic acid (SA)- and jasmonic acid (JA)-pathway-related genes were activated by A. spiraecola saliva infiltration. However, two SA-pathway-related genes were activated and three JA-pathway-related genes were suppressed following A. citricidus saliva infiltration. Treatment with boiled saliva did not similarly impact D. citri feeding behavior or plant defense response. This study suggests that salivary components (those that can be inactivated by heating) from two citrus aphid species differently affect plant defenses and that they were responsible for the contrasting plant-mediated effects of two citrus aphids on the feeding behavior of D. citri. This study indicates a novel three-way citrus aphid–plant–citrus psyllid interaction.

show abstract

A Transcriptomic and Proteomic Analysis of the Diaphorina citri Salivary Glands Reveals Genes Responding to Candidatus Liberibacter asiaticus

Cited by 11 publications

References 39 publications

Chromosome‐level genome assembly of the Asian citrus psyllid, Diaphorina citri

Chromosome‐level genome assembly of the Asian citrus psyllid, Diaphorina citri

You are what you eat—ecological niche and microhabitat influence venom activity and composition in aquatic bugs

Saliva-Mediated Contrasting Effects of Two Citrus Aphid Species on Asian Citrus Psyllid Feeding Behavior and Plant Jasmonic Acid Pathway

Contact Info

Product

Resources

About