Proteinease inhibition, membrane stabilization, antioxidant and phytochemical evaluations of leaves, seeds and calyces of four selected edible medicinal plants

Kambizi, Learnmore; Bakare-Odunola, M. T.; Oladiji, Adenike Temidayo; Kola-Mustapha, Adeola Tawakalitu; Amusa, T.O.; Atolanı, Olubunmi; Njinga, Ngaitad S.; Quadri, A.L.

doi:10.1080/23312009.2017.1314064

Cited by 13 publications

(9 citation statements)

References 42 publications

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“…Membrane stabilising activity showed that PCs/SOLU inhibit the lysis of erythrocytes. In general, leucocytes migrates to the inflammation site in response to the stimulus which plays a vital role during inflammatory disorders [46,47]. The PCs/SOLU nanodispersion ointment lowers the occurrence of radicals (super oxide) by preventing the leucocytes migration at the inflammation site.…”

Section: Membrane Stabilizing Activitymentioning

confidence: 99%

Nano formulated proanthocyanidins as an effective wound healing component

Ramesh

Volova

Oluwafemi

et al. 2020

Materials Science and Engineering: C

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Proanthocyanidins (PCs), a component of grape seed extract (GSE), has recently being used for the treatment of wounds. However, poor absorption, poor stability and rapid elimination from the systemic circulation limit its acceptance. In addressing these problems, we herein report the development of PCs based nanoformulations (PCs/SOLU) for the first time based on 1 % GSE and assessed its wound healing potential in-vivo on the wistar rats. GSE and PCs/SOLU nanodispersions 1 % were prepared by incorporating them into the ointment base via uniform mixing to form ointment which could be easily applied topically to wounds. The antibacterial activity of PCs/SOLU against gram positive and gram-negative bacteria strains proved that the cell membranes became more permeable with disrupted cell structure. While carrageenan and histamine induced rat paw edema analyses show there was no inflammatory signs in animals treated with 1 wt% of PCs/SOLU nanodispersion. Excision wound measuring about 3 cm in depth was created on the wistar rats. The ointment was applied topically on the wounded site and the wound contraction was measured daily. Grape seed extract (GSE) ointment, ointment base and povidone-iodine (Povi-Iod) ointment of about 1 % was used as the control, positive and negative standards. PCs/SOLU nanodispersion heals the wound by mobilising the fibroblasts in the wound site and inhibits the inflammatory response through decreased expression of monocyte. The macroscopical, immunological and histopathological assessments revealed that PCs/SOLU nanodispersion ointment usage improves the cell adhesion and proliferation

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Section: Membrane Stabilizing Activitymentioning

confidence: 99%

Nano formulated proanthocyanidins as an effective wound healing component

Ramesh

Volova

Oluwafemi

et al. 2020

Materials Science and Engineering: C

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“…The pulverized calyces (808 g) of HS were soaked in 20% (w/v) cold distilled water for 24 h. The extract was filtered using muslin and then filtered again using Whatman No 1 filter paper. The resulting filtrate was concentrated over water bath at 40 °C ( Kambizi et al., 2017 ) and weighed. The concentrated extract was then stored at +4 °C until required for analysis.…”

Section: Methodsmentioning

confidence: 99%

Toxicity assessment of sub-acute and sub-chronic oral administration and diuretic potential of aqueous extract of Hibiscus sabdariffa calyces

Njinga

Kola-Mustapha

Quadri

et al. 2020

Heliyon

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Background: Food and herbal usage of Hibiscus sabdariffa (HS) is attaining improved global relevance and acceptance without recourse to its potential toxic effects. This study investigated the safety profile of acute, sub-acute, subchronic administrations and diuretic potential of aqueous extract of Hibiscus sabdariffa calyces (AEHSC). Method: Acute oral toxicity, sub-acute and sub-chronic toxicity as well as diuretic studies were carried out on HS. A total of 20 Wistar rats were used for each toxicity study and assigned into four groups of five rats. The extract was administered as a single daily dose of 125, 250 and 500 mg/kg body weight (bwt) for 28 and 90 days respectively. To evaluate diuretic activity, 25 rats were divided into five groups of five rats and administered normal saline, hydrochlorothiazide 10 mg/kg, AEHSC 67.5, 125 and 250 mg/kg via the oral route. Urine sample was collected after 18 h, volume measured and concentration of electrolytes analyzed. The hematological and biochemical parameters were evaluated as well as the histopathology of kidney and liver. Results: The acute oral toxicity was found to be >2000 mg/kg. AEHSC did not alter concentration of WBC, MCV, MCHC, lymphocyte as well as total and direct bilirubin in the sub-acute study. However, AEHSC significantly (p < 0.05) increased total protein, albumin, globulin, Na þ , Cl À , HCO 3 and platelet levels, while levels of uric acid, creatinine, K þ , RBC, Hb, total cholesterol, triglycerides, LDL-C, HDL-C and atherogenic index were decreased significantly (p < 0.05). In the sub-chronic study, AEHSC significantly (p < 0.05) increased the levels of globulin, urea, creatinine, MCH and atherogenic index. The concentrations of uric acid, WBC, platelets and HDL-C were significantly (p < 0.05) decreased. In both the sub-acute and sub-chronic studies, activities of ALP, ALT, AST, GGT and LDH in selected organs were altered without significant increase (P < 0.05) in activity of these enzymes in the serum. The AEHSC at all the doses showed remarkable diuretic activity during 18 h period comparable to hydrochlorothiazide. The extract also showed a non-dose-dependent increase in excretion of electrolytes. Histological analysis of sections of the liver and kidney for both sub-acute and sub-chronic studies showed normal histology comparable to the control group. Conclusion: This study revealed AEHSC has some toxic effects in rats on sub-chronic administration. In addition, the extracts produced a significant diuretic activity. Hence, prolonged oral consumption of the extract may not be recommended.

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“…2013; 2018; Kambizi et al. 2017). Briefly, 7 mM ABTS in deionized water was mixed at ratio 1:1 with freshly prepared potassium persulfate (2.45 mM) and the mixture kept in the dark for 24–48 h. The ABTS solution was diluted in methanol at 1:25.…”

Section: Methodsmentioning

confidence: 99%

“…The procedure described previously for estimating anti-inflammatory potential (Kambizi et al. 2017) was adopted. Mice were kept for 2 weeks without any contact with nonsteroidal anti-inflammatory drugs.…”

Section: Methodsmentioning

confidence: 99%

Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil

Atolanı

Oguntoye

Areh

et al. 2019

Pharmaceutical Biology

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Context: Cola gigantea A. Chev. (Sterculiaceae) is an important medicinal tropical flora. Objective: The seed oil of C. gigantea , an underutilized tropical plant was investigated for its antioxidant, anti-inflammatory, anti- Toxoplasma, and cytotoxicity activities as well as the chemical composition. Materials and methods: The physicochemical parameters of the seed oil obtained via Soxhlet extraction was determined while the fatty acid and non-fatty acid component were analyzed by gas chromatography-mass spectrometry. The antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays (10–50 µg/mL) while the anti-inflammatory property was determined through Cell Membrane Stabilization assay. The anti-parasite and cytotoxicity activity were evaluated (0–1000 µg/mL) using Toxoplasma gondii and mammalian cell line assays, respectively. Results: The oil had fatty acids which ranged from C-12 to C-23 with linoleic (18:2) and palmitic acids (16:0) being dominant. The oil had 89.41% unsaturated fatty acids with sterolic acid, an uncommon acetylenic fatty acid reported for the first time. Non-fatty acids obtained include cholesterol (2.12%), campesterol (14.12%), stigmasterol (34.07%) and β-sitosterol (49.68%). The oil had a significantly ( p < 0.05) low scavenging activity against DPPH radicals (IC 50 > 50 µg/mL) compared with ascorbic acid. In contrast, the oil showed better activity against ABTS radicals (IC 50 44.19 ± 6.27 µg/mL) compared with ascorbic acid or quercetin. Furthermore, the oil showed anti- T. gondii and dose-dependent cytotoxicity in HFF cells with selectivity index (IC 50 /EC 50 < 1). Discussion and conclusions: The antioxidant potential of the oil suggests that it may serve as a potential source for various preparations for pharmaceuticals and cosmeceuticals.

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Proteinease inhibition, membrane stabilization, antioxidant and phytochemical evaluations of leaves, seeds and calyces of four selected edible medicinal plants

Cited by 13 publications

References 42 publications

Nano formulated proanthocyanidins as an effective wound healing component

Nano formulated proanthocyanidins as an effective wound healing component

Toxicity assessment of sub-acute and sub-chronic oral administration and diuretic potential of aqueous extract of Hibiscus sabdariffa calyces

Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil

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