Nano formulated proanthocyanidins as an effective wound healing component

Abstract

Proanthocyanidins (PCs), a component of grape seed extract (GSE), has recently being used for the treatment of wounds. However, poor absorption, poor stability and rapid elimination from the systemic circulation limit its acceptance. In addressing these problems, we herein report the development of PCs based nanoformulations (PCs/SOLU) for the first time based on 1 % GSE and assessed its wound healing potential in-vivo on the wistar rats. GSE and PCs/SOLU nanodispersions 1 % were prepared by incorporating them into the ointment base via uniform mixing to form ointment which could be easily applied topically to wounds. The antibacterial activity of PCs/SOLU against gram positive and gram-negative bacteria strains proved that the cell membranes became more permeable with disrupted cell structure. While carrageenan and histamine induced rat paw edema analyses show there was no inflammatory signs in animals treated with 1 wt% of PCs/SOLU nanodispersion. Excision wound measuring about 3 cm in depth was created on the wistar rats. The ointment was applied topically on the wounded site and the wound contraction was measured daily. Grape seed extract (GSE) ointment, ointment base and povidone-iodine (Povi-Iod) ointment of about 1 % was used as the control, positive and negative standards. PCs/SOLU nanodispersion heals the wound by mobilising the fibroblasts in the wound site and inhibits the inflammatory response through decreased expression of monocyte. The macroscopical, immunological and histopathological assessments revealed that PCs/SOLU nanodispersion ointment usage improves the cell adhesion and proliferation

Skin wound healing assisted by angiogenic targeted tissue engineering: A comprehensive review of bioengineered approaches

“…Grape seeds contain a combination of lipids, proteins, carbohydrates, and polyphenols, the percentages of which depend on the type of grapes. Polyphenols presented in the grape seed include tannins, gallic acid, monomeric flavan‐3 catechin, epicatechin 3‐galit, and dimeric, monomeric and polymeric proanthocyanidin which can mitigate the chronic wounds complications 181‐183 …”

A sensitive and smartphone colorimetric assay for the detection of hydrogen peroxide based on antibacterial and antifungal matcha extract silver nanoparticles enriched with polyphenol

“…One of the most promising developments of nanotechnolgy is the evaluation of preservative efficacy of nanoadditives, which have been gaining attention for preventing from pathogenic microbial contamination during the novel fabrication of cosmetics [ 24 – 27 ]. Recently, metal/metal oxide-based nanoformulations are promising nanosized protectants against several pathogens in food, cosmetic, agriculture and drug with their excellent antimicrobial and antifungal properties [ 28 – 32 ]. Among these materials, green synthesized silver-based nanostructures are widely used to control bacterial growth in biomedical applications [ 33 ].…”

Preparation and Characterization of Nanofibrous Scaffolds of Ag/Vanadate Hydroxyapatite Encapsulated into Polycaprolactone: Morphology, Mechanical, and In Vitro Cells Adhesion

“…Skin is a vital organ that protects the human body against outer pathogens [1][2][3], and thus it is a crucial part of the immune system [4][5][6]. Additionally, skin displays a key role in the human body metabolic cycle [7,8].…”