A sensitive and smartphone colorimetric assay for the detection of hydrogen peroxide based on antibacterial and antifungal matcha extract silver nanoparticles enriched with polyphenol

Abstract

Current trends in scientific studies focus on the development of smartphone-based biosensors via green nanoparticle for clinical diagnosis, food, and environmental monitoring. In this study, we developed a novel portable smartphone-based biosensor via green dendrimer-coated matcha extract/silver nanoparticles (ME-Ag NPs) enriched with polyphenol for detecting hydrogen peroxide (H 2 O 2 ). Also, we investigated the biological evaluation of the nanostructure as a safe preservative for use in biomedical applications. Ag NPs were prepared using a green sonochemical method and were characterized to determine surface and chemical properties by different techniques such as scanning electron microscopy—energy-dispersive X-ray, transmission electron microscope, Fourier transform infrared spectroscopy, atomic force microscopy, X-ray diffraction, and Brunauer–Emmett–Teller. Furthermore, antimicrobial and antifungal properties of ME-Ag NPs were investigated against pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aureginosa, Escherichia coli, Candida albicans, and Aspergillus brasiliensis. The experimental sensor methodology was based on the detection of H 2 O 2 by analysis of images of novel silver nanostructure-coated papers and processing of color histograms with a RGB (red–green–blue) analyzer software. Consequently, the smartphone-based biosensor exhibited high sensitivity with detection limits of 0.82 μM response time of 5 s. The smartphone-based biosensor via ME-Ag NPs provided a rapid and selective detection of H 2 O 2 .

Matcha‐silver nanoparticles reduce gamma radiation‐induced oxidative and inflammatory responses by activating SIRT1 and NLRP‐3 signaling pathways in the Wistar rat spleen

“…AgNPs that were enhanced with polyphenols were created using the technique outlined by Tan et al 23 Silver nitrate (AgNO 3 ) was dissolved in 200 mL of deionized water, amounting to 0.32 g. The mixture was then agitated for 10 min at 25°C. In 10 mL of distilled water, 0.016 g of NaOH was dissolved, and the mixture was agitated for 10 min at 25°C.…”

“…AgNPs that were enhanced with polyphenols were created using the technique outlined by Tan et al 23 Silver nitrate (AgNO 3 ) was dissolved in 200 mL of deionized water, amounting to 0.32 g.…”

“…Using matcha green tea extract as a reducing and stabilizing substance, sonochemically produced AgNPs with sizes ranging from 10 to 30 nm. 23 Japanese green tea in powder form, known as matcha, has recently become more and more popular around the world. 24 When matcha is grown in the traditional manner, the synthesis and accumulation of biologically active compounds, such as theanine, caffeine, chlorophyll, and other kinds of catechins, are improved.…”

“…Nebulization and cavitation are the physical processes involved in sonochemistry. Using matcha green tea extract as a reducing and stabilizing substance, sonochemically produced AgNPs with sizes ranging from 10 to 30 nm 23 …”

The microbial‐killing Ag nanoparticles in food supply chain: How it was applied and what a consumer should know?

“…Results showed that the detection of H 2 O 2 using this novel nanosensor is highly selective and rapid. (Tan et al, 2022) (Continues)…”