Protein release kinetics for core–shell hybrid nanoparticles based on the layer-by-layer assembly of alginate and chitosan on liposomes

Haidar, Ziyad S.; Hamdy, Reggie C.; Tabrizian, Maryam

doi:10.1016/j.biomaterials.2007.11.012

Cited by 239 publications

(195 citation statements)

References 37 publications

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“…For formulations F10, F11, F12, F14, and F15, the drug release constants obtained for the zero-order drug release kinetics suggested that liposomes acted as reservoir systems for a continuous Ser-HCl delivery. The literature provides evidence that for liposomal formulations, the diffusion model based on the goodness-of-fit test endures to be the most appropriate model to describe the kinetics of drug release, when compared to zero-order and first-order (36). Furthermore, it enables an understanding of the quantitative deviation of the proposed formulation from the diffusion-controlled ideal Higuchi model.…”

Section: Drug Leakagementioning

confidence: 99%

Glycosylated Sertraline-Loaded Liposomes for Brain Targeting: QbD Study of Formulation Variabilities and Brain Transport

et al. 2016

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Abstract. Effectiveness of CNS-acting drugs depends on the localization, targeting, and capacity to be transported through the blood-brain barrier (BBB) which can be achieved by designing brain-targeting delivery vectors. Hence, the objective of this study was to screen the formulation and process variables affecting the performance of sertraline (Ser-HCl)-loaded pegylated and glycosylated liposomes. The prepared vectors were characterized for Ser-HCl entrapment, size, surface charge, release behavior, and in vitro transport through the BBB. Furthermore, the compatibility among liposomal components was assessed using SEM, FTIR, and DSC analysis. Through a thorough screening study, enhancement of SerHCl entrapment, nanosized liposomes with low skewness, maximized stability, and controlled drug leakage were attained. The solid-state characterization revealed remarkable interaction between SerHCl and the charging agent to determine drug entrapment and leakage. Moreover, results of liposomal transport through mouse brain endothelialpolyoma cells demonstrated greater capacity of the proposed glycosylated liposomes to target the cerebellar due to its higher density of GLUT1 and higher glucose utilization. This transport capacity was confirmed by the inhibiting action of both cytochalasin B and phenobarbital. Using C6 glioma cells model, flow cytometry, time-lapse live cell imaging, and in vivo NIR fluorescence imaging demonstrated that optimized glycosylated liposomes can be transported through the BBB by classical endocytosis, as well as by specific transcytosis. In conclusion, the current study proposed a thorough screening of important formulation and process variabilities affecting brain-targeting liposomes for further scale-up processes.

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Section: Drug Leakagementioning

confidence: 99%

Glycosylated Sertraline-Loaded Liposomes for Brain Targeting: QbD Study of Formulation Variabilities and Brain Transport

et al. 2016

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“…These associated macromolecules have been shown to transport through mucosa and epithelia more efficiently [165]. Cationic chitosan in combination with other natural polymers has been shown to enhance the drug encapsulation efficiency of liposomes via the layer-by-layer (L-b-L) self-assembly technique [166]. Nanoparticles made of chitosan in association with polyethylene oxide have been used as protein carrier [167].…”

Section: Drug Delivery Carriersmentioning

confidence: 99%

Is Chitosan a New Panacea? Areas of Application

Castro¹,

Paulín²

2012

The Complex World of Polysaccharides

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Chitin is a big molecule composed of -beta-1,4-N-acetylglucosamine (GlcNAc) monomers. There are three forms of chitin: , , and chitin. The -form, is mainly obtained from crab and shrimp. Both and chitin/chitosan are commercially available [5]. Sources of chitinIn the book "Chitin" published by Muzarelli in 1977, we can find a complete list of organisms that contain chitin: Fungi, Algae, Cnidaria (jellyfish), Aschelminthes (round worm), Entoprocta, Bryozoa (Moss or lace animals, Phoronida (Horseshoe worms), Brachiopoda(Lamp shells), Echiruda, Annelida (Segmented worms), Mollusca, Arthropoda and Ponogophora [6]). Herring, P.J in 1979 wrote that chitin is the main component of arthropod exoskeletons, tendons, and the linings of their respiratory, excretory, and digestive systems, as well as insects external structure and some fungi. It is also found in the reflective material (iridophores) of both epidermis and eyes of arthropods and cephalopods (phylum: Mollusca) and the epidermal cuticle of the vertebrate Paralipophrys trigloides (fish) is also chitinous [7,8]. The main commercial sources of chitin are the shell wastes of shrimp, lobsters, crabs and krill. There are three forms of chitin: , , and chitin. The -form, is composed of alternating antiparallel polysaccharide strands and is mainly obtained from crab and shrimp. -Chitin is by far the most abundant; it occurs in fungal and yeast cell walls, krill, lobster and crab tendons and shells, shrimp shells, and insect cuticle. The rarer -chitin is composed of parallel strands of polysaccharides, is found in association with proteins in squid pens [9,10] and in the tubes synthesized by pogonophoran and vestimetiferan worms [11,12]. It also occurs in aphrodite chaetae [13] as well as in the lorica, built by some seaweeds or protozoa [14,15,16]. And 2 parallel chains alternating with an antiparallel strand constitute gamma chitin and are found in fungi [15].

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“…In our laboratory, we have successfully completed all the in vitro characterization, formulation ( Figure 11) and release kinetics studies (Haidar et al, 2008;Haidar et al, 2010a) of our NP system. Then, we showed the capability of the novel core-shell NPs to efficiently encapsulate and release a range of concentrations of rhBMP7 up to 45 days (Haidar et al, 2009a) (Figure 12).…”

Section: Development Of a Delivery System For Bmpsmentioning

confidence: 99%